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1.
BMC Res Notes ; 11(1): 921, 2018 Dec 22.
Article in English | MEDLINE | ID: mdl-30577879

ABSTRACT

OBJECTIVE: Hilsa shad (Tenualosa ilisha), is a popular fish of Bangladesh belonging to the Clupeidae family. An anadromous species, like the salmon and many other migratory fish, it is a unique species that lives in the sea and travels to freshwater rivers for spawning. During its entire life, Tenualosa ilisha migrates both from sea to freshwater and vice versa. DATA DESCRIPTION: The genome of Tenualosa ilisha collected from the river Padma of Rajshahi, Bangladesh has been sequenced and its de novo hybrid assembly and structural annotations are being reported here. Illumina and PacBio sequencing platforms were used for high depth sequencing and the draft genome assembly was found to be 816 MB with N50 size of 188 kb. MAKER gene annotation tool predicted 31,254 gene models. Benchmarking Universal Single-Copy Orthologs refer 95% completeness of the assembled genome.


Subject(s)
Fishes/genetics , Genome/genetics , Animals , Bangladesh , Fresh Water , Seawater
2.
Nature ; 422(6934): 859-68, 2003 Apr 24.
Article in English | MEDLINE | ID: mdl-12712197

ABSTRACT

Neurospora crassa is a central organism in the history of twentieth-century genetics, biochemistry and molecular biology. Here, we report a high-quality draft sequence of the N. crassa genome. The approximately 40-megabase genome encodes about 10,000 protein-coding genes--more than twice as many as in the fission yeast Schizosaccharomyces pombe and only about 25% fewer than in the fruitfly Drosophila melanogaster. Analysis of the gene set yields insights into unexpected aspects of Neurospora biology including the identification of genes potentially associated with red light photobiology, genes implicated in secondary metabolism, and important differences in Ca2+ signalling as compared with plants and animals. Neurospora possesses the widest array of genome defence mechanisms known for any eukaryotic organism, including a process unique to fungi called repeat-induced point mutation (RIP). Genome analysis suggests that RIP has had a profound impact on genome evolution, greatly slowing the creation of new genes through genomic duplication and resulting in a genome with an unusually low proportion of closely related genes.


Subject(s)
Genes, Fungal/genetics , Genome, Fungal , Neurospora crassa/genetics , Calcium Signaling/genetics , DNA Methylation , Diterpenes/metabolism , Evolution, Molecular , Gene Duplication , Heterotrimeric GTP-Binding Proteins/metabolism , Multienzyme Complexes/genetics , Multigene Family/genetics , Mutagenesis/genetics , Neurospora crassa/cytology , Neurospora crassa/enzymology , Neurospora crassa/metabolism , Plant Diseases/microbiology , RNA Interference , RNA, Ribosomal/genetics , Receptors, Cell Surface/genetics , Repetitive Sequences, Nucleic Acid , Sequence Analysis, DNA , Signal Transduction/genetics
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