[Function of hormone-secreting cells cultured under continuous perfusion with nutrient medium]. / Funktsiia gormonsekretiruiushchikh kletok, kul'tiviruemykh v usloviiakh nepreryvnoi perfuzii pitatel'noi sredoi.

The data are presented as to prolactin and ACTH secretion by rat adenohypophysis cells and insulin secretion by pancreatic beta-cells of the newborn rats during cell cultivation on flat synthetic membranes with pores differing in diameter under continuous medium perfusion. The cultured cells were discovered to be viable and to possess secretory activity. Secretion of prolactin and ACTH and that of insulin in the appropriate cultures increases in the course of long-term incubation under continuous medium perfusion. It is concluded that application of the porous membranes enables a selective study of the release of one or another hormone into the medium. It is believed that cultivation of hormone-secreting and hormone-susceptible cells under closed- and unclosed-type nutrient medium perfusion is fairly promising.

[Procedure for producing a monolayer primary culture af rat hepatocytes and evaluation of its functional activity]. / Sposob polucheniia monosloinoi pervichnoi kul'tury gepatotsitov krys i otsenka ee funktsional'noi aktivnosti.

A simple method for isolating hepatocytes from the liver with the aid of trypsin and their cultivation is described. The functional activity of the primary monolayer culture of hepatocytes was evaluated from the rate of albumin synthesis and secretion. The basal rate of albumin synthesis was fairly high during the first 10 minutes but after 2 hours decreased and reached plateau. On the contrary, the basal rate of albumin secretion rose with time and after 2 hours attained the rate of albumin synthesis. Human growth hormone (500 ng/ml) stimulated albumin output within 3 hours of incubation with hormonal stimulation in the cells from hypophysectomized rats being more pronounced as compared to that seen in hepatocytes from intact animals. A conclusion is made that the hepatocyte culture prepared by the method described can produce a release albumin and retains sensitivity to the hormonal regulator.

[Effect of growth hormone on polynucleotide phosphorylase activity in a primary monolayer culture of rat hepatocytes]. / Vliianie gormona rosta na aktivnost' polinukleotidfosforilazy v pervichnoi monosloinoi kul'ture gepatotsitov krys.

Growth hormone (1 microgram/ml) inoculated into the primary culture of rat hepatocytes and incubated for 3 hours at 37 degrees C decreases 2-fold the activity of polynucleotide phosphorylase (PNPase). In the culture of hepatocytes from hypophysectomized rats, the basal activity of PNPase is significantly higher as compared with that in the culture of hepatocytes from intact animals. The most pronounced inhibitory effect of growth hormone on PNPase was seen in the culture of hepatocytes from hypophysectomized animals.