ABSTRACT
Changes in the light transmission coefficient of human adipose cells as a result of photodynamic action have been detected and investigated. Based on the statistical processing of digital micrographs of thin tissue slices, the kinetics of processes occurring in the adipose tissue has been quantitatively estimated. It has been suggested that the effects observed are brought about through the lipolysis of adipose cells with the retention of their structure.
Subject(s)
Adipocytes/radiation effects , Adipose Tissue/radiation effects , Obesity/drug therapy , Photochemotherapy , Adipocytes/drug effects , Adipose Tissue/drug effects , Humans , Image Processing, Computer-Assisted , Kinetics , Light , Lipolysis/radiation effects , Microtomy , Optical Devices , Photosensitizing Agents/pharmacology , Quaternary Ammonium Compounds/pharmacology , Staining and LabelingABSTRACT
Changes in optical properties of human adipose tissue cells after photodynamic exposure in vitro were found and investigated. Analysis of the kinetics of the process was realized by means of photomicrography of the object investigated. The statistical computer processing of digital photos obtained gave us an opportunity to estimate quantitatively the kinetics of photodynamic effect upon the tissue. Optical interpretation of the photos obtained indicates that the observed phenomenon corresponds to the partial lysis of adipose tissue cells without their complete destruction.
