[Ion-sensitive endogenous regulators of Na+,K+-ATPase obtained from epithelium of rat small intestine]. / Ionchutlyvi endohenni rehuliatory Na+,K+-ATP-azi, oderzhani z epiteliiu tonkoï kyshky shchura.

From epithelial layer of rat intestinal were selected water soluble substances which influenced on Na+,K(+)-ATPase activity in 2 different ways: substances with molecular weight 220 Da, 400 Da were its activators, substance with weight 150 Da-inhibitor. Na+,K(+)-ATPase activators preincubated previously with Na+ acquire properties of inhibitors. Bivalent cations Ca, Mg remove this effect of Na-ions.

[The role of the thrombocyte-activating factor in inhibiting Na+,K(+)-ATPase in different tissues after ischemia and reperfusion of the small intestine]. / Rol' trombotsitaktiviruiushchego faktora v ingibirovanii Na+,K(+)-ATP-azy v razlichnykh tkaniakh posle ishemii i reperfuzii tonkoi kishki.

Activity of transport Na+, K(+)-ATP-ase has been studied both in the ischemia-affected proximal section of the small intestine and in tissues which did not endure ischemia (the brain, renal cortex and the distal part of the small intestine) in dynamics 5, 30 and 60 min, after reperfusion of the ischemized small intestine of rats. It is shown that after 30 min-long ischemia of the small intestine, the Na+, K(+)-ATP-ase activity is inhibited in all the tissues mentioned. Maximal inhibition of the enzyme activity after 30 min long reperfusion is 74, 34, 94 and 58% for the brain, renal cortex, proximal and distal sections of the small intestine, respectively. It has been found that the blood serum from ischemized rats also exerts an inhibitory effect on the enzyme activity. Application of inhibitor of the thrombocyte-activating factor (TAF) BN52021 has permitted demonstrating its role in the protecting effect under conditions of Na+, K(+)-ATP-ase inhibition in the post-Ischemic period and inhibitory effect of the blood serum from ischemized rats on the enzyme activity. A conclusion is made that the TAF participated in the inhibitory effect of Na+, K(+)-ATP-ase activity in different tissues after ischemia of the small intestine of rats.

[An analysis of the physiological activity of organic compounds of ozokerite obtained by its biotransformation by naftusia water microorganisms]. / Analiz fiziolohichnoï aktyvnsti orhanichnykh spoluk ozokerytu, otrymanykh ioho biotransformatsiieiu mikroorhanizmamy vody "Naftusia".

A possibility to obtain physiologically active substances by means of biotransformation of natural carbohydrate raw material-ozokerite using the saprophite groups of microorganisms isolated from the medicinal water naftusia has been studied. It is shown that microorganisms metabolise ozokerite with following formation of the whole spectrum of water-soluble substances, activators and inhibitors of Na, K-ATPase, cholopoiesis stimulators being found among them. A conclusion has been made that using microorganisms inhabiting water naftusia can obtain from ozokerite organic substances conditioning physiologic activity of this medicinal water.

[The effect of a lyophilized extract of the mucosa of the proximal small intestine on kidney water- and salt-excretory functions in rats]. / Vplyv liofilizovanoho ekstraktu slyzovoï obolonky proksymal'noho viddilu tonkoï kyshky na vodo- ta solevydil'nu funktsiï nyrok u shchuriv.

Experiments on Wistar rats injected intragastrically deionized water (1 % of the body weight) and intra-abdominally 0.1 mg/kg of the lyophilized water extract (LWE) from the thin intestine have shown that under these conditions diuresis and excretion of K+ with the urine increase and retention of Na+ excretion decreases. After intragastric injection of isotonic NaCl solution, the LWE has exerted only the K-excretion effect. An increase in the LWE doses from 1 to 10 mg/kg has weakened all these reactions. It has been found in experiments in vitro that the LWE has exerted an activatory dose-dependent effect on Na, K-ATPase from the kidney cortex cells.

[The use of Smecta for the treatment of the basic digestive tract diseases]. / Primenenie smekty dlia lecheniia osnovnykh zabolevanii pishchevaritel'nogo kanala.

The indices for the gastric contents aggression and protection ratio returned to normal in patients with peptic ulcer and preulcerous condition during the treatment with smekta due to a decrease in proteolytic activity and increase in the synthesis of the mucus protective proteins. Monitoring of intragastric pH in the body of the stomach throughout 24 hours in patients with peptic ulcer of duodenal location revealed significant acid-neutralizing effect of smekta. Patients with nonspecific ulcerous colitis enjoyed freedom of side effects as a result of the use of salofalk and derived full therapeutic benefit from this treatment. Under the effect of smekta there occurred suppression of the growth of pathogenic proteolytic flora, improvement of qualitative properties of coli flora and growth of sugar-lytic flora in patients with intestinal dysbiosis. According to the data available, smekta strengthens the mucous barrier by improving the conformation of the mucus structural elements.

[Release of an endogenous Na+, K+-ATPase into the blood during hypoosmotic stimulation of the mucosa of the gastrointestinal tract in rats]. / Vysvobozhdenie v krov' éndogennogo aktivatora Na+, K+-ATP-azy pri gipoosmoticheskoi stimuliatsii slizistoi zheludochno-kishechnogo trakta krys.

An endogenous activator (EA) of Na+, K(+)-ATPase was found in the rat blood serum in the in vivo experiments. EA appeared after intragastric introduction of mannitol hypotonic (20 mmol/l) solution (1.5% b. w.) and activated enzyme of the intact rat enterocytes. EA retained its effect on Na+, K(+)-ATPase after heating during 10 min up to 100 degrees C. This endogenous activator is thermally stable and belongs to low molecular compounds with the molecular weight of 10 kDa. It is supposed that EA can participate in the water-salt humoral regulation after loading the organism with hypoosmotic solutions.

[Effect of hypoosmotic stimulation of the gastrointestinal mucosa on the activity of NA+, K+-atpase of epithelial cells in the small intestine and kidney in rats]. / Vplyv giposmotychnoi stymuliatsii slyzovoi poverkhni shlunkovo- kyshkovogo traktu na aktyvnist Na+, K+- ATFazy v klitynakh epiteliiu tonkoi kyshky ta nyrok shchuriv.

The experiments on Wistar rats have shown that intragestral injection with mannitol hypotonic solution (20 mmol/l) causes the significant activation of Na+, K(+)-ATPase of duodenum and distal intestine epithelial cells, kidney cortex cells, but does not affect the brain cortex of Na+, K(+)-ATPase activity. Simultaneously the activator of enzyme (AE) enters blood serum of rats, its activity is revealed by blood serum addition to homogenates of tissues of control rats. It is assumed that AE is produced in duodenal and intestinal mucosa released to blood after stimulation of mucosal surface by hypotonic solutions and included into the osmoregulation processes on the Na+, K(+)-ATPase level.

[The osmotic regulation of gastrin secretion by the isolated rat duodenum]. / Osmoticheskaia reguliatsiia sekretsii gastrina izolirovannoi dvenadtsatiperstnoi kishki krysy.

Activating effect of osmotic irritant agent was suppressed by N- and M-cholinoblocking agents, benzohexonium and pyrenzepine in the rat isolated duodenum. The same was true for phentolamine and not for obsidan. The data obtained suggest that the osmotic regulation of the gastrin secretion is performed through the mechanism of a local intramural reflex with participation of N- and M-cholinoreactive and alpha-adrenoreactive agents.

[Secretion of endogenous regulators of Na K ATPase by isolated rat duodenum in osmotic stimulation of its mucous membrane]. / Sekretsiia endohennykh reguliatoriv Na+, K+-ATFazy izol'ovanoiu dvanadtsaiatypaloiu kyshkoiu shchuriv pid vplyvom osmotychnoï stymuliatsiï ïï slizovoï obolochki.

The experiments in vitro have shown that perfusion of the isolated duodenum cavity in rats by hypotonic (20 mosmol/l H2O) or hypertonic (500 mosmol/l H2O) solutions of NaCl and mannitol during 10 min stimulates secretion of endogenic factors (activators or inhibitors of enterocytes of Na+, K(+)-ATPase) into the serous incubation solution. Contact of the duodenum mucose surface with isotonic (300 mosmol/l H2O) solutions does not stimulate the secretion of this enzyme regulators. Endogenic substance are thermostable: they retain activity relative to Na+, K+ ATPase of enterocytes after heating (100 degrees C, 10 min duration) of serous incubation solutions.

[Osmotic actions on the epithelial Na,K pump of the small intestine]. / Osmoticheskie vozdeistviia na Na,K-nasos épiteliia tonkoi kishki.

Intragastric administration of mannitol or NaCl hypotonic solution induced a significant shift of the electrolyte balance in the small intestine epithelium, and activated the enterocytes' Na,K pump in Wistar rats. De-ionised water caused the maximal enhancement of the Na,K-ATPase activity, whereas hypertonic solutions exerted the opposite effect, a substance-inhibitor appearing in the blood serum. Similar results were obtained in vitro. The possibility of the Na,K-pump regulation by enterocytes and the role of small intestine in secretion of an endogenous factor regulating this enzyme's activity, is discussed.

[The gastrin-secreting effects of the salt components of naftusia water]. / Gastrininkretornye éffekty solevykh komponentov vody naftusi.

Intragastric administration of certain salt components of mineral water naftusia in minimal concentrations (1-5 mmol/l) changes intensity and direction of hypergastrinemic reaction in the rats. The effect is determined by the anionic composition of salts rather than by the cationic one and depends on the initial concentration of gastrin in blood.

[The depressant effect of fatty acids on the electrical and contractile activities of smooth muscles]. / Ugnetaiushchee vliianie zhirnykh kislot na élektricheskuiu i sokratitel'nuiu aktivnost' gladkikh myshts.

Inhibitory action of some fatty acids on the visceral and vascular smooth muscle of guinea pig and rat was studied by the sucrose-gap method. Potency of the inhibitory effects of fatty acids depended both on the carbon chain length and degree of saturation of acid molecule. The revealed inhibitory effect of some fatty acids (caprinic and oleinic) can be of essential physiological significance in regulation of excitation of smooth muscles.

[Mineral water Naftusia as a xenobiotic]. / Mineral'naia voda naftusia kak ksenobiotik.

It is stated that organic matters contained in mineral water Naftusia appear in blood serum 10 min after intragastric introduction of this water to rats. Long administration of water Naftusia to animals stimulates the monooxygenase hepatic system and secretory renal apparatus, that probably, underlies the mechanism for weakening of damaging effect of hepatotropic poison.

[Direct and indirect effects of mineral water Naftusia and its components on the sodium-potassium pump of the small-intestinal epithelium in rats]. / Priamoe i oposredovannoe deistvie mineral'noi vody naftusi i ee komponentov na natrii-kalievyi nasos épiteliia tonkoi kishki krysy.

Experiments on rats have shown that intragastric single introduction of mineral water Naftusia to animals in a dose of 1.5% of the body weight of animals induces sodium accumulation in the small intestine epithelium, that is a result of Na(+)-, K(+)-pump inhibition by fatty acids of this water. Naftusia absorption induces appearance of inhibitors of Na(+)-, K(+)-ATPase enzymic system in blood serum of rats. Artificial salt Naftusia analog (ASNA) has induced contrary changes in the water-electrolytic balance and activated Na(+)-, K(+)-ATPase. Blood serum of ASNA-loaded rats acquired ability to activate Na(+)-, K(+)-pump of epitheliocytes in intact rats.

[The composition and antianemia efficacy of the iron-containing waters of the Skhodnitsa spring]. / Sostav i antianemicheskaia éffektivnost' zhelezosoderzhashchikh vod skhodnitskogo mestorozhdeniia.

Slight-mineralized chalybeate waters of the Skhodnitsa deposit containing organic matters are distinguished by high-molecular organic iron complexes present in waters. It is shown that such natural complex iron compounds are characterized by high biological accessibility making these mineral waters very useful for posthemorrhagic anemia due to their therapeutic action.

[The role of osmotic factors in the effect of mineral water Naftusia on smooth muscles of the portal vein]. / Rol' osmoticheskogo faktora v deistvii mineral'noi vody naftisi na gladkie myshtsy vorotnoi veny.

The effect of the Krebs solution tonicity on the electrical and mechanical activity of smooth muscles has been studied while adding 1-5% volume of mineral water Naftusya, its artificial salt analog, and distilled water into the above solution. It is shown that effects observed are induced by the medium tonicity changes rather than by biologically active components of the mineral water. The smooth muscle cells of the rat portal vein are sensitive osmometers which affect hypotonic Krebs solution by 1%.