ABSTRACT
Serotonin and histamine are important components of neurohumoral regulation. The biologic and fluorometric methods currently used for their measurements are labor-consuming and involve the use of unavailable reagents and expensive equipment. The authors suggest a sensitive simplified technique for serotonin and histamine measurements in a small volume of the biologic material (tissue and blood). The procedure is as follows: serotonin and histamine are isolated by thin-layer chromatography, the obtained samples are treated with alloxan in dimethyl formamide medium, and the stained solutions are examined in spectrophotometer. The suggested method is highly specific and sensitive, simple, makes use of Soviet equipment and of available reagents, this recommending it for clinical biochemical, as well as for experimental studies.
Subject(s)
Histamine/analysis , Serotonin/analysis , Animals , Chromatography, Thin Layer , Intestinal Mucosa/analysis , Rats , Spectrometry, FluorescenceABSTRACT
Within 6 months after decentralization of the rat and dog intestine, signs of reinnervation appear. Hypersecretion of the intestine juice, a decrease in the acetylcholine and noradrenaline contents, reduction of the invertase and alkaline phosphatase activity in the juice and homogenate of the small intestine occur during first weeks. The activating effect of the food on transmitter-enzymatic processes weakens whereas activity of the enteral nervous system is enhanced. The data obtained are significant for intestine transplantation research.
Subject(s)
Intestinal Mucosa/metabolism , Intestinal Secretions/metabolism , Intestine, Small/innervation , Acetylcholine/analysis , Alkaline Phosphatase/metabolism , Animals , Cholinesterases/metabolism , Denervation , Monoamine Oxidase/metabolism , Nerve Regeneration , Norepinephrine/analysis , Rats , Sucrase/metabolismSubject(s)
Intestinal Mucosa/drug effects , Pentagastrin/pharmacology , Secretin/pharmacology , Animals , RatsABSTRACT
Chronic experiments in dogs with isolated loop of the small intestine and acute experiments in rats revealed specific interrelationships between the gastrin and the cholinergic activity: pentagastrin potentiated its effects by activation of cholinergic processes in the intestinal mucosa (increasing the acetylcholin content and the cholinesterase activity). The hormone activates the intestinal juice and enzyme secretion as well. The invertase and alkaline phosphatase activities intensify in the dense portion of the juice and in the tissue homogenate. Pentagastrin seems to exert a regulating effect upon the ecbolic processes in the ileum mucosa.