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1.
Vopr Virusol ; 37(1): 31-7, 1992.
Article in Russian | MEDLINE | ID: mdl-1413710

ABSTRACT

Reproduction of parental strains and reassortants (with known genome composition) of influenza A and B viruses was studied in chick embryos (CE) and in different cell lines (SPEV, MDCK, BHK-21, M22, etc.). The results agree with the concept that the yield of influenza A virus in CE depends on its M-gene. At the same time, the experimental results suggest that reproduction of influenza B virus in the same system is not determined by M-gene. Reproduction (hr-phenotype) of influenza A and B viruses in cell cultures was shown to be determined not only by the gene coding for hemagglutinin but also by other virus genes, the reproduction level being dependent on different genes in different cell systems.


Subject(s)
Genome, Viral , Influenza A virus/physiology , Influenza B virus/physiology , Reassortant Viruses/physiology , Virus Replication/physiology , Animals , Cell Line , Humans , Influenza A virus/genetics , Influenza B virus/genetics , Reassortant Viruses/genetics , Virus Cultivation , Virus Replication/genetics
2.
Vopr Med Khim ; 30(5): 65-72, 1984.
Article in Russian | MEDLINE | ID: mdl-6528523

ABSTRACT

Rabbit mesenteric lymph contained all the main classes of lipoproteins detected in plasma: chylomicrons (CM), very low density lipoproteins (VLDL), low density lipoproteins (LDL), high density lipoproteins (HDL). The main classes of lipoproteins in lymph were CM and VLDL, whereas LDL and HDL were the main lipoproteins in blood plasma. The heaviest subfractions HDL3 were not observed in mesenteric lymph. Lymph contained only low amount of LDL; molecular mass of lymphatic LDL was lower as compared with the blood plasma lipoproteins. Mesenteric lymph lipoproteins were enriched with triglycerides and contained less cholesterol, phospholipids and protein than the corresponding blood plasma lipoproteins. As distinct from blood plasma lipoproteins, apo E and apoproteins of low molecular mass (apo C?) were not found in lymphatic lipoproteins. Content of Apo-I was much higher in lymphatic VLDL as compared with the blood plasma VLDL. The dissimilarity in patterns and composition between blood plasma and lymphatic lipoproteins indicated that the latter were derived from intestine. Thus, intestine was able to produce CM, VLDL, HDL2 and apparently a small amount of LDL.


Subject(s)
Lipoproteins/blood , Lymph/analysis , Mesentery/analysis , Animals , Apolipoproteins/analysis , Apolipoproteins/blood , Cholesterol/analysis , Cholesterol/blood , Chylomicrons/analysis , Chylomicrons/blood , Electrophoresis, Disc , Lipoproteins/analysis , Lipoproteins, LDL/analysis , Lipoproteins, LDL/blood , Lipoproteins, VLDL/analysis , Lipoproteins, VLDL/blood , Male , Rabbits , Triglycerides/analysis , Triglycerides/blood
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