[Determination of the molecular weight of microbial polysaccharides using high performance exclusion chromatography]. / Opredelenie molekuliarnykh mass mikrobnykh polisakharidov s pomoshch'iu vysokoéffektivnoi ékskliuzionnoi khromatografii.

An automatic method of determining the molecular weight parameters (Mw, Mn) of microbial polysaccharides such as dextran, pullulan was developed based on the use of high performance size-exclusion chromatography on the two types of columns: Zorbax PSM 60 + 300 + 1000 and SynChropack GPC 100 + 500 + 1000. The Mw and Mn values were determined for a number of domestic and foreign dextran preparations. Changes in the molecular weight of pullulan and hydroxyethylstarch resulted from acid and enzymatic hydrolysis were estimated.

[Physico-chemical and biological properties of a new plasma substitute based on hydroxyethylated starch]. / Fiziko-khimicheskie i biologicheskie svoistva novogo plazmozamenitelia na osnove oksiétilirovannogo krakhmala.

Samples of hydroxyethylstarch of M 100-160 kDa, Mn 50-60 kDa and substitution degree 0.6-0.7 were prepared and characterized. Hydroxyethylstarch was shown to be susceptible to cleavage by amylolytic enzymes. All samples of hydroxyethylstarch at 2-4% concentration were compatible with perfluorohydrocarbon emulsion "Perftoran" and exhibited high haemodynamic efficiency. The 6 and 10% solutions of hydroxyethylstarch in 0.9% aqueous sodium chloride normalized haemodynamics in massive blood losses. Hydroxyethylstarch was completely removed from blood-stream. The preparations were shown to be nontoxic.

[Comparative study of the pullulans synthesized by Pollularia (Aureobasidium) pullulans strains of different ploidies]. / Sravnitel'noe izuchenie pullulanov, sinteziruemykh shtammami Pullularia (Aureobasidium) pullulans raznogo urovnia ploidnosti.

Pullularia pullulans strains of different ploidy synthesize pullulans similar in their characteristics to those described in literature. These are glucans whose glucose residues are linked with alpha(1 leads to 4) and alpha(1 leads to 6) bonds in the proportion of 2.2:1. The pullulans differ from one another in their water solubility, molecular mass and in the ability to be cleaved by alpha-amylase and dextranase. The minor structural modifications of pullulan molecules in the polyploid strains as compared to the pullulan synthesized by the parent haploid culture are caused, apparently, by mutations induced with mitotic poisons.