ABSTRACT
The results of the study of the phagocytic element of immunity in cases of primary immunodeficiency, made with the use of a complex of functional tests, are presented. As shown in this study, in patients with definitely characterized phagocytic defect (chronic granulomatous disease) a decrease in all parameters under study, with the exception of phagocyte index, is observed. In patients with immunodeficient states of nonphagocytic nature moderate mosaic deviations of phagocytosis characteristics are noted. For further and more profound study of phagocytosis disturbances in such patients, it is expedient, in particular, to carry out studies in the activity of myeloperoxidaze and the intensity of oxygen-dependent metabolism. The use of the complex of methods used in this investigation has shown their high diagnostic value. It may be recommended for clinical practice under the condition of strict standardization of experimental techniques.
Subject(s)
Immunologic Deficiency Syndromes/immunology , Phagocytosis/immunology , Adolescent , Blood Bactericidal Activity/immunology , Child , Child, Preschool , Humans , Immunity, Cellular/immunology , Immunologic Deficiency Syndromes/blood , Immunologic Deficiency Syndromes/etiology , Luminescent Measurements , Neutrophils/immunology , Oxygen/blood , Peroxidase/bloodSubject(s)
Agammaglobulinemia/diagnosis , Immunization, Passive/methods , Immunoglobulins/metabolism , T-Lymphocytes/immunology , Agammaglobulinemia/metabolism , Agammaglobulinemia/therapy , Child, Preschool , Female , Humans , Immunization, Passive/adverse effects , Immunoglobulins/drug effects , Infant , Male , T-Lymphocytes/drug effectsSubject(s)
Granulomatous Disease, Chronic/diagnosis , Child , Child, Preschool , Combined Modality Therapy , Drug Therapy, Combination , Female , Granulomatous Disease, Chronic/complications , Granulomatous Disease, Chronic/therapy , Humans , Immunologic Deficiency Syndromes/complications , Immunologic Deficiency Syndromes/diagnosis , Immunologic Deficiency Syndromes/therapy , Immunotherapy , Infant , Luminescent Measurements , Lymphocyte Subsets/immunology , Male , Respiratory Burst/drug effects , ZymosanABSTRACT
There were 3 groups of children with recurrent purulent infections, who were entered into randomized clinical studies. Group 1 received sodium nucleinate, group 2 diucifon and group 3 placebo (calcium gluconate). After 6 months a well-defined clinical effect manifested by the lowering of the rate and gravity of exacerbations was recorded in 68% of the patients belonging to groups 1 and 2 and in 39% of group 3 patients, which is much more seldom (p less than 0.05). During immunological studies according to the level I tests, no significant changes in the indicators were recorded either before or after the treatment both among the patients who responded to immunotherapy and among those who did not respond to it. The conclusion is drawn that if indicated clinically, sodium nucleinate of diucifon can be administered in spite of the normal immune status in accordance with the level I tests.
Subject(s)
Adjuvants, Immunologic/therapeutic use , Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Infections/drug therapy , Nucleic Acids/therapeutic use , RNA, Fungal , Sulfones/therapeutic use , Uracil/analogs & derivatives , Adolescent , Age Factors , Child , Child, Preschool , Drug Combinations , Female , Humans , Infant , Male , Recurrence , Suppuration , Uracil/therapeutic useABSTRACT
A set of 2n basis lymphocyte subpopulation, distinguishing by the presence of at least one antigen, may be composed for a given combination of n surface antigens. The relative amounts of basis subpopulations constitute a thin subpopulation structure of lymphocytes. General methods of lymphocyte phenotyping using one (or two) differentiation antigen give actually the estimation of a sum of basis subpopulations expressing a given antigen (or some combination of two antigens). The mathematical method was elaborated for reconstruction of the thin subpopulation structure by fitting the relative assemblage of biparametric cytofluorograms. The proposed approach was used for the subpopulation analysis of lymphocytes from patients with different forms of immunological deficiency.
Subject(s)
Lymphocytes/ultrastructure , Antibodies, Monoclonal , Antigens, Differentiation/analysis , Fluoroimmunoassay/instrumentation , Fluoroimmunoassay/methods , Humans , Immunologic Deficiency Syndromes/blood , Lymphocytes/immunology , Mathematics , Models, Biological , PhenotypeABSTRACT
Altogether 79 children with primary immunodeficiency conditions (PIC) and 21 children suffering from frequently occurring bacterial infections were examined for the level of rosette-forming cells and lymphocytes reacting positively with monoclonal antibodies to different phenotypic membranous markers (SD-antigens and others). The patients with combined forms of immunodeficiency, with T deficiency playing the key role manifested a significant lowering of the content of the subpopulations of SD3+, SD5+, SD7+ and SD4+ lymphocytes and the decrease of the SD4/SD8 ratio. The the reduction of the number of B lymphocytes (smIg + K1, SD19 +), the low levels of Ia+ -cells, and the decrease of the SD4/SD8 at the expense of the content of SD8+ -cells. In the patients with selective immunodeficiency and recurrent bacterial infections without verified PIC, these parameters did not undergo any essential changes. As compared with immunofluorescent assays, the measurement of E-RFL and M-RFL amounts was found to have insufficient information content for the diagnosis of PIC.
