Subject(s)
COVID-19 , Ritonavir , Humans , Adult , Ritonavir/therapeutic use , Cost-Effectiveness Analysis , Outpatients , COVID-19 Drug Treatment , Lactams , Nitriles , Antiviral Agents/therapeutic useABSTRACT
BACKGROUND AND AIM: MicroRNAs are small non-coding RNAs that play important regulatory roles in a variety of biological processes, including complex metabolic processes, such as energy and lipid metabolism, which have been studied in the context of diabetes and obesity. Some particular microRNAs have recently been demonstrated to abundantly and stably exist in serum and to be potentially disease-specific. The aim of this profiling study was to characterize the expression of miRNA in serum samples of obese, nonobese diabetic and obese diabetic individuals to determine whether miRNA expression was deregulated in these serum samples and to identify whether any observed deregulation was specific to either obesity or diabetes or obesity with diabetes. PATIENTS AND METHODS: Thirteen patients with type 2 diabetes, 20 obese patients, 16 obese patients with type 2 diabetes and 20 healthy controls were selected for this study. MiRNA PCR panels were employed to screen serum levels of 739 miRNAs in pooled samples from these four groups. We compared the levels of circulating miRNAs between serum pools of each group. Individual validation of the twelve microRNAs selected as promising biomarkers was carried out using RT-qPCR. RESULTS: Three serum microRNAs, miR-138, miR-15b and miR-376a, were found to have potential as predictive biomarkers in obesity. Use of miR-138 or miR-376a provides a powerful predictive tool for distinguishing obese patients from normal healthy controls, diabetic patients, and obese diabetic patients. In addition, the combination of miR-503 and miR-138 can distinguish diabetic from obese diabetic patients. CONCLUSION: This study is the first to show a panel of serum miRNAs for obesity, and compare them with miRNAs identified in serum for diabetes and obesity with diabetes. Our results support the use of some miRNAs extracted from serum samples as potential predictive tools for obesity and type 2 diabetes.
Subject(s)
Diabetes Mellitus, Type 2/genetics , MicroRNAs/genetics , Obesity/genetics , Adult , Aged , Biomarkers/blood , Case-Control Studies , Diabetes Mellitus, Type 2/blood , Diabetes Mellitus, Type 2/complications , Diabetes Mellitus, Type 2/pathology , Female , Gene Expression Profiling , Gene Expression Regulation , Humans , Male , MicroRNAs/blood , Middle Aged , Obesity/blood , Obesity/complications , Obesity/pathology , Predictive Value of Tests , ROC CurveABSTRACT
El cuadro clínico de la endocarditis infecciosa es inespecífico. La fiebre y la auscultación de un soplo cardíaco son los síntomas más frecuentes. La asociación de artralgias es relativamente común, y es inusual la presentación en forma de poliartritis. Presentamos un caso de endocarditis infecciosa que se inicia con clínica subaguda de fiebre y poliartritis (AU)
Clinical signs of infective endocarditis are inespecific. Fever and heart murmur are the most frequent symptoms. Arthralgiass association is fairly common but presentation as polyarthritis is inusual. We report a cse of infective endocarditis that begins with subacute fever and polyarthritis (AU)
Subject(s)
Humans , Male , Adult , Endocarditis, Bacterial/complications , Arthritis/etiology , Endocarditis, Bacterial/diagnosis , Fever/etiology , Enterococcus faecalis/isolation & purification , Anti-Bacterial Agents/therapeutic useABSTRACT
La enfermedad cardiovascular es común en la artritis reumatoide. No obstante, la afección miocárdica es inusual y suele cursar de forma asintomática. Presentamos un caso de miocardiopatía dilatada en una paciente con artritis reumatoide e insuficiencia cardíaca progresiva que requirió su inclusión en lista de trasplante cardíaco (AU)
Cardiovascular pathology is common in rheumatoid arthritis. However, myocardial affection is unusual and clinical disease is rare. We report a case of dilated cardiomyopathy in a patient with rheumatoid arthritis and progressive heart failure that required inclusion into a heart transplantation list (AU)
Subject(s)
Humans , Cardiomyopathies/complications , Arthritis, Rheumatoid/complications , Heart Transplantation , Waiting Lists , Heart Failure/complications , Rheumatoid Factor/isolation & purification , Cardiomyopathies/diagnosisABSTRACT
Cardiovascular pathology is common in rheumatoid arthritis. However, myocardial affection is unusual and clinical disease is rare. We report a case of dilated cardiomyopathy in a patient with rheumatoid arthritis and progressive heart failure that required inclusion into a heart transplantation list.
ABSTRACT
Clinical signs of infective endocarditis are inespecific. Fever and heart murmur are the most frequent symptoms. Arthralgias's association is fairly common but presentation as polyarthritis is inusual. We report a cse of infective endocarditis that begins with subacute fever and polyarthritis.
