ABSTRACT
Triterpenoids of ursane class: ursolic acid, ilelatifol D, corosolic acid and euscaphic acid were isolated for the first time from Leonurus cardiaca, a member of the family Lamiaceae. The isolated compounds were tested for their cell-based antiinflammatory potential by suppressing respiratory burst activity and superoxide scavenging property by using xanthine/xanthine oxidase system to produce superoxides in the cell-free system. Ursolic acid was found to be an excellent inhibitor for the superoxides produced in the cellular system, while the same was inactive in the superoxide scavenging activity in cell-free system.
Subject(s)
Free Radical Scavengers/pharmacology , Respiratory Burst/drug effects , Superoxides/antagonists & inhibitors , Triterpenes/pharmacology , Cells, Cultured , Dose-Response Relationship, Drug , Free Radical Scavengers/chemistry , Humans , Lamiaceae/chemistry , Molecular Structure , Neutrophils/cytology , Neutrophils/drug effects , Neutrophils/metabolism , Superoxides/metabolism , Triterpenes/chemistry , Xanthine Oxidase/metabolism , Ursolic AcidABSTRACT
A new sesquiterpene lactone, nubiol (1), belonging to the guaiane class, along with a dimer, bisnubidiol (2), has been isolated from Salvia nubicola collected from Quetta, Pakistan. The structures of both metabolites were elucidated with the aid of spectroscopic techniques including 2D-NMR. Additionally, the structure of nubiol (1) was finally confirmed via the single crystal X-ray diffraction. Nubiol (1) was found moderately active against Pseudomonas aeruginosa.
Subject(s)
Cycloheptanes/chemistry , Lactones/isolation & purification , Salvia/chemistry , Sesquiterpenes, Guaiane/chemistry , Sesquiterpenes/chemistry , Crystallography, X-Ray , Lactones/chemistry , Magnetic Resonance Spectroscopy , Models, Molecular , Molecular StructureABSTRACT
A new epimeric lupane triterpenoid, 3alpha-hydroxy-20-oxo-30-norlupane (1), and a new flavanone (nubatin; 3) have been isolated from Salvia nubicola collected from Quetta, Pakistan. The structures of these constituents were elucidated with the aid of spectroscopic techniques, including two-dimensional NMR and NOE experiments. Both the metabolites were found to be moderately bio-active.
