[Effect of the pesticide carbaryl (sevin) on the course of experimental Erysipelothrix rhusiopathiae infection in rats]. / Vliianie na pestitsida karbaril (sevin) vurkhu khoda na eksperimentalna infektsiia u plukhove s Erysipelothrix rhusiopathiae.

Rats treated with carbaril (sevin) at the rate of 2 to 5 mg were infected via the feed with Erysipelothrix rhusiopathiae in the course of 30 days. Studied were the clinical picture, some paraclinical indices, the mortality rate, and the morphologic and histopathologic changes in the parenchymal organs. It was found that rats that were given carbaril for a long time in minimum amounts and were then subjected to infection developed a characteristic clinical picture and showed a high mortality rate (nearly twice as high as that with the control animals). In hemocultures the causative agent was isolated over a longer period than in the case with the controls. Both the gross and the histopathologic findings were more strongly manifested in the test (carbaril-treated) rats.

[Effect of different doses of lysine on the free amino acid content of the blood serum]. / Vliianie na razlichni dozi lizin vurkhu sudurzhanieto na svobodnite aminokiselini v kruvniia serum.

Investigations were carried out of the effect of various doses of lysine in the feed (6, 12, 24, and 30 g per fodder unit) on the amount of the total proteins, hemoglobin, and free amino acids in the blood serum. It was found that the free amino acids in the blood serum of sheep directly correlated with the content of lysine in the diet. The addition of 6 g lysine per fodder unit supplied the most adequate amount of amino acids in the body to meet the metabolic requirements. The concentration of free lysine in the serum remained at a comparatively/relatively constant level (2.5 mg%), the amount of total protein and hemoglobin was highest (within the range of the physiologic norms), and the general status of the animals proved best. The higher rates of lysine (24 and 30 g per fodder unit) led to an abrupt change in the concentration of all free amino acids in the serum, most strongly being affected glycine, aspartic acid, glutamic acid, alanine, and leucin. The amount of free amino acids (lysine) in the blood serum rose parallel to the increase in the lysine doses in the ration of sheep. At 30 g lysine per fodder unit there was a fivefold rise as against the control group.

[Amino acid makeup of beef]. / Prouchvaniia vurkhu aminokiselinniia sustav na govezhdo meso.

Studies were carried out both on the quantitative and qualitative composition of aminoacids in the proteins of m. longissimus dorsi from cattle of the "Bulgarian Brown" breed with the help of an automatic aminoanalyzer Hd 1200 E, made in Czechoslovakia. Hydroxyproline and triptophane were determined at different analytical speeds. It was established that in the proteins of the muscle investigated are contained the following aminoacids, expressed in g/16 g N: lysine--7.77; treonine--4.09, valine--5.12, methyonine--2.50, isoleucine--4.67, leucine--8.42, phenylalanine--4.34, triptophane--1.40, histidine--3.37, arginine--6.17, aspartic acid--7.92, serine--3.31, glutamine acid--13.30, proline--3.73, glycine--4.79, alanine--4.99, tirosine--3.84 and hydroxyproline--0.59. The quantity of the essential aminoacids in the proteins of the muscle investigated from cattle, except the value of methyonine and cystine (the method we implemented does not secure the determination of cystine), surpasses the norm for their contents in the proteins suggested by FAO/WHO.

[Amino acid composition of lamb]. / Prouchvaniia vurkhu aminokiselinniia sustav na ovcho meso.

Studies were carried out both on the quantitative and qualitative composition of aminoacids in the proteins of n. longissimus dorsi, from sheep of the "Blackheaded Pleven" breed. For the purpose was used an automatic aminoanalyzer of 1200 E, made in Czechoslovakia. Hydroxyproline and triptophane were determined in separate analytical pathways. It was established that in the proteins of the muscle investigated are contained the following aminoacids, expressed in grams /16 g N: lysine--7,90; treonine--3,92; valine--4,84; methyonine--2,72; isoleucine--4,51; leucine--7,54; phenylalanine--4,64; triptophane--1,39; histidine--3,02; arginine--6,09; aspartic acid--7,16; serine--3,64; glutamine acid--11,49; proline--3,54; glycine--4,29; alanine--4,65; tirosine--2,94 and hydoxyproline--0,42. A similarity was proved in the aminoacid composition of m. longissimus dorsi from sheep with that of the same muscle with cattle and swine.

[Action of Bacitrafen Premix on layers and broilers]. / Prouchvane deistvieto na preparata batsitrafen premiks pri kokoshki nosachki i pileta broileri.

Observations were made concerning the effect of Bacitraphen-Premix, consumed by poultry with their food. It was proved that similarly to other nutritive antibiotics, the preparation agreed well with them. It was also proved that it does not show toxicity--LD0 and LD50 were higher than the maximal does applied. Bacitraphen-Premix had a positive effect on the egg-laying abilities of layers, as well as on the fecundity ability and incubation qualities of eggs. It showed a definite antistress effect due to which it can be successfully used as a prophylactic means in poultry-raising in case of stress reactions.

Nutrition effect on liver and lysosomes. VIII. The undernourishment role in a following short-term starvation.

91 male, albino rats, Wistar strain, with body weight 150 +/- 10 g, subdivided into 2 series were investigated. The 1rst series animals (E) were fed on single daily meals in the course of 20 days, only 2 h in 24 h and after that period were subjected to complete starvation and investigated on the 24th, 48th, 72th and 96th h resp. after the ceasing of food supply. A 2nd animal series (C) serves as a control, which in the course of 20 days, were on a all-round diet and later put in identical conditions of complete starvation and also investigated on the 24th, 48th, 72th and 96th h. The liver histological, histo-enzymatical (AP, BG, AlkP and ATP) and electron microscopic changes followed up, as well as certain physiological, biochemical and morphometric indices. The dynamics of the alterations in complete starvation and the confrontation of the data between the 2 animal series with different preceding nutritional regimes show that: 1) single daily meals leads to real undernourishment; 2) the undernourishment predetermines a more severe course of the following period of complete starvation; 3) 3 phases are established during the complete starvation: adaptive, alterative-restorative and alterative. Each phase bears a definite subcellular characteristics where the lysosomes apparatus plays an essential role.

[Effect of a ration with a varying amount of fat and protein of plant and synthetic origin on some indices of ruminal content and of the blood in lactating sheep]. / Vliianie na dazhbi s razlichno kolichestvo maznini i protein ot rastitelen i sinteticgen proizkhod vurkhu niakoi pokazateli v turbukhovoto sudurzhanie i kruvta pri laktirashti ovtse

The pH value of the rumen content in lactating ewes given sunflower expeller was 7.10, and that in ewes given urea was 6.70. The amount of ammonia in the rumen of ewes fed expeller was at a dependable lower level than the amount of ammonia with ewes fed urea. In animals fed expeller with the ration the blood sugar, protein, and lipids were higher, while acetone, beta-oxibutyric acid, and cholesterin dropped. In urea feeding, the levels of acetone, betaoxibutyric acid, and cholesterin rose, while blood sugar, protein, and lipids were lower. The Ca, P, Na, K, and Fe contents as well as the activity of the SGOT and SGPT enzymes in the blood of lactating ewes did not change essentially except for the amounts of Mg and Cu.