ABSTRACT
For the development of the excimer probe responsible for DNA target, the deoxyuridine phosphoramidite derivative bearing the silylated pyrene attached at the C-5 position was prepared and incorporated into oligonucleotides. The modified oligonucleotides showed the excimer emission in the absence of the target DNA, on the other hands, the excimer emission was quenched in the presence of the target DNA. For the utilization of the fluorescence behavior, the novel molecular beacon probe containing the silylated pyrene-modified nucleoside at the stem region was designed and the fluorescence property of the probe found to show the responsibility for DNA target.
Subject(s)
DNA/analysis , Deoxyuridine/chemistry , Drug Design , Fluorescent Dyes/chemistry , Oligonucleotide Probes/chemistry , Organophosphorus Compounds/chemistry , Pyrenes/chemistry , Fluorescent Dyes/chemical synthesis , Molecular Structure , Oligonucleotide Probes/chemical synthesisABSTRACT
Preparation and properties of the novel fluorescently labelled oligonucleotides, containing the silylated pyrene derivatives are reported. The silylated pyrene derivatives were introduced into both 5'-terminus and C-5 position of the deoxyuridine derivatives. The fluorescent spectra indicated the possibility of the discrimination of the duplex formation by the simple denaturation.
