ABSTRACT
A cytogenetic analysis of 14 primary testicular nonseminomatous germ cell tumors has been carried out after short term tissue culture. The modal chromosome numbers ranged from 53 to 113, in agreement with flow cytometric determination of the DNA content of the tumors. At least one copy of an i(12p) was present in 12 tumors. Two tumors, however, lacked that marker. Some chromosomes are apparently overrepresented, whereas others are underrepresented, although some differences between seminomas and nonseminomas were noticed.
Subject(s)
Chromosome Aberrations/pathology , Neoplasms, Germ Cell and Embryonal/genetics , Testicular Neoplasms/genetics , Analysis of Variance , Aneuploidy , Chromosome Disorders , Humans , Karyotyping , Male , Neoplasms, Germ Cell and Embryonal/pathology , PloidiesABSTRACT
Seventeen soft tissue sarcomas were karyotyped and the chromosomal findings were compared with those in the literature. Some cases were easily classified (immuno)histologically, whereas others defied classification. In four cases with unequivocal histology, a "characteristic" chromosomal abnormality was found. In one case a diagnosis that was doubtful on histologic grounds was supported by the chromosomal findings, whereas in three other cases, the chromosomal pattern led to reconsideration of the histologic diagnosis. The remaining cases either showed chromosomal abnormalities with hitherto undescribed breakpoints or breakpoints described in tumors with different histology. The results extend the knowledge of chromosomal abnormalities in soft tissue sarcomas. Most importantly, the present approach demonstrates that close collaboration between the surgical pathologist and the cytogeneticist may improve the classification of soft tissue sarcomas.
Subject(s)
Histiocytoma, Benign Fibrous/genetics , Liposarcoma/genetics , Sarcoma, Synovial/genetics , Soft Tissue Neoplasms/genetics , Adolescent , Adult , Aged , Aged, 80 and over , Child , Chondrosarcoma/genetics , Chondrosarcoma/pathology , Female , Histiocytoma, Benign Fibrous/pathology , Humans , Karyotyping , Leiomyosarcoma/genetics , Leiomyosarcoma/pathology , Liposarcoma/pathology , Male , Middle Aged , Osteosarcoma/genetics , Osteosarcoma/pathology , Sarcoma, Ewing/genetics , Sarcoma, Ewing/pathology , Sarcoma, Synovial/pathology , Soft Tissue Neoplasms/pathologyABSTRACT
A cytogenetic analysis of 13 mature residual teratomas following chemotherapy revealed modal chromosome numbers ranging from 52 to 85, in agreement with the flow cytometric determination of the DNA content of the tumors. At least one copy of an i(12p) was present in 12 tumors. One tumor, however, lacked that marker. The comparison between the chromosomal abnormalities found in mature residual teratomas following chemotherapy and those from primary testicular nonseminomas suggests that residual teratomas result from selection of clones from the primary tumor with a less abnormal karyotype.
Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Chromosome Aberrations , Chromosome Disorders , Teratoma/genetics , Testicular Neoplasms/genetics , Adult , Flow Cytometry , Humans , Karyotyping , Male , Middle Aged , Teratoma/drug therapy , Testicular Neoplasms/drug therapyABSTRACT
Cytogenetic analysis was performed of three seminomas, two primary nonseminomas, and two mature residual teratomas following chemotherapy, all lacking i(12p). Testicular germ cell tumors without an i(12p) may represent a subgroup of germ cell tumors, also in their clinical course, compared with those having i(12p).
Subject(s)
Chromosome Aberrations , Chromosomes, Human, Pair 12 , Neoplasms, Germ Cell and Embryonal/genetics , Testicular Neoplasms/genetics , Adult , Chromosome Banding , Genetic Markers , Humans , Karyotyping , Male , Middle Aged , Neoplasms, Germ Cell and Embryonal/classification , Teratoma/genetics , Testicular Neoplasms/classificationABSTRACT
The cytogenetics of 12 cases of renal adenocarcinoma are presented. Clonal abnormalities were found in nine patients. Worth mentioning are abnormalities of the X chromosome (1 X) and the chromosomes #1(3 X), #3(3 X), #7(4 X), #8(4 X), and #14(2 X). From our data and data from the literature it appeared that the chromosomal regions 3p11-p21, 1q21, 14q22, and Xp11 are important for the oncogenesis of renal cell carcinoma as well as increased dosage of chromosome #7 and loss or abnormalities of chromosomes #8 and #14.
Subject(s)
Carcinoma, Renal Cell/genetics , Chromosome Aberrations , Kidney Neoplasms/genetics , Adolescent , Adult , Aged , Child , Child, Preschool , Chromosome Banding , Female , Genetic Markers , Humans , Karyotyping , Male , Middle AgedSubject(s)
B-Lymphocytes/physiology , Immunoglobulin kappa-Chains/genetics , Leukemia/genetics , Animals , Antibodies, Monoclonal , Antigens, Surface/analysis , B-Lymphocytes/pathology , Cell Differentiation , Cytoplasm/immunology , Immunoglobulin kappa-Chains/analysis , Immunoglobulin mu-Chains/analysis , Karyotyping , Leukemia/immunology , Leukemia/pathology , Rats , Receptors, Antigen, B-Cell/analysis , Recombination, GeneticABSTRACT
Lymphocyte cultures from the Chinese hamster were made with lymphocytes isolated with Ficoll-Isopaque or with Haemaccel. In some experiments the red blood cells (r.b.c.) that remained after lymphocyte isolation were lysed. Optimum phytohaemagglutinin (PHA) concentration for lymphocyte proliferation depended upon the manner in which the lymphocytes were obtained. Lower PHA concentrations were needed for optimum stimulation of lymphocytes isolated with Ficoll-Isopaque than for lymphocytes isolated with Haemaccel. Lysis of the r.b.c. remaining after isolation of lymphocytes with Haemaccel resulted in a decrease of optimum PHA concentration. The optimum PHA concentration depended strongly on r.b.c. contamination, the higher the r.b.c. contamination the higher the optimum PHA concentration. However, only part of the differences found in optimum PHA concentrations can be attributed to r.b.c. contamination.
Subject(s)
Erythrocytes , Lymphocyte Activation , Lymphocytes/immunology , Phytohemagglutinins , Animals , Cells, Cultured , Cricetinae , Culture Media , Culture Techniques/methods , Dose-Response Relationship, DrugABSTRACT
Chinese hamster lymphocyte cultures were supplemented with foetal calf serum (FCS) or hose serum (HS). Addition of 2-mercaptoethanol (2-ME) to cultures supplemented with these sera resulted in a marked increase lymphocyte proliferation. The PHA concentrations necessary for optimal lymphocyte stimulation were much lower in cultures supplemented with HS than with FCS. Phytohaemagglutinin (PHA) concentrations optimal for stimulation of a given number of lymphocytes in cultures supplemented with FCS often gave inhibition of similar cultures supplemented with HS.
Subject(s)
Blood , Fetal Blood , Lymphocyte Activation , Phytohemagglutinins/pharmacology , Animals , Cattle , Cells, Cultured , Cricetinae , Cricetulus , Culture Media , Dose-Response Relationship, Immunologic , Female , Horses , Immunologic Techniques , Mercaptoethanol/pharmacology , PregnancyABSTRACT
Cell hybridization was done between a monolayer of B14-150 Chinese hamster cells and a suspension of either mouse leukemia cells or normal human lymphocytes. Cell contact was obtained by centrifugation of the suspension cells onto the monolayer cells in a culture plate. Cell fusion was done by means of polyethylene glycol (PEG). The optimum time for PEG exposure as well as the yield of hybrid cells differed markedly with the different combinations.
