ABSTRACT
Anti-allergic effects (types I and IV) of the 70% ethanol extract (CM-ext) obtained from Cnidii Monnieri Fructus (dried fruits of Cnidium monnieri) were investigated on 48 h homologous passive cutaneous anaphylaxis (PCA), 2, 4-dinitrofluorobenzene (DNFB)-induced contact dermatitis and picryl chloride (PC)-induced contact dermatitis in experimental animals. CM-ext showed inhibitory effects on these allergic models. Osthol isolated from CM-ext also had the inhibitory effects. These results suggested that Cnidii Monnieri Fructus might be useful as an agent for allergic diseases and that its anti-allergic effect was partially attributable to a coumarin derivative, osthol.
Subject(s)
Anti-Allergic Agents/pharmacology , Cnidium/chemistry , Coumarins/pharmacology , Fruit/chemistry , Animals , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Coumarins/chemistry , Dermatitis, Contact/pathology , Dermatitis, Contact/prevention & control , Dinitrofluorobenzene/antagonists & inhibitors , Dinitrofluorobenzene/toxicity , Diphenhydramine/pharmacology , Magnetic Resonance Spectroscopy , Male , Mice , Mice, Inbred ICR , Passive Cutaneous Anaphylaxis/drug effects , Picryl Chloride/antagonists & inhibitors , Picryl Chloride/toxicity , Plant Extracts/pharmacology , Prednisolone/pharmacology , Pruritus/chemically induced , Rats , Rats, Wistar , Skin/pathologyABSTRACT
Antipruritic effects of 70% ethanol extract (CM-ext) of Cnidii Monnieri Fructus (dried fruits of Cnidium monnieri CUSSON, Umberifferae) were investigated. In mice, an oral administration of CM-ext (200 and 500 mg/kg) inhibited compound 48/80-induced scratching behavior without influence on spontaneous locomotion. Isopimpinellin (3) and osthol (1), coumarin derivatives isolated from CM-ext, showed an inhibitory effect on compound 48/80-induced scratching behavior.
