Metabolic Biomarkers of Red Beetroot Juice Intake at Rest and after Physical Exercise.

BACKGROUND: Red beetroot is known to be a health-promoting food. However, little attention is placed on intestinal bioactive compound absorption. The aim of the study was to assess the urinary red beetroot juice (RBJ) intake biomarkers and possible differences in RBJ's micronutrient absorption at rest or after physical exercise. METHODS: This is a three-armed, single-blind study, involving seven healthy volunteers which were randomly divided into three groups and alternatively assigned to three experimental sessions: RBJ intake at rest, RBJ intake with physical activity, and placebo intake with physical activity. For each session, urine samples were collected before and 120, 180, and 240 min after the intake of RBJ or placebo. The same sampling times were employed for the experimental session at rest. The RBJ metabolic composition was also characterized to identify the urinary biomarkers derived from the intake. RESULTS: 4-methylpyridine-2-carboxylic acid, dopamine-3-O-sulfate, glutamine, and 3-hydroxyisobutyrate were identified as RBJ intake biomarkers. Physical activity significantly increased only the dopamine-3-O-sulfate excretion 120 min after RBJ intake. CONCLUSIONS: Urinary dopamine-3-O-sulfate is related to RBJ dopamine content, while 4-methylpyridine-2-carboxylic acid is a betanin or betalamic acid catabolite. The different excretions of these metabolites following physical activity suggest a possible effect on the RBJ uptake depending on different transport processes through the mucosa, namely diffusion-mediated transport for dopamine and saturable transcellular transport for betalamic acid derivatives. These results open new perspectives in improving the absorption of natural bioactive molecules through physical activity.

The Impact of a 14-Day Altitude Training Camp on Olympic-Level Open-Water Swimmers' Sleep.

Despite the common belief that sleep quality at altitude is poor, the scientific evidence to support this notion is still modest. Therefore, the purpose of the present study was to evaluate possible changes of actigraphy-based and subjective sleep parameters in a group of elite open-water swimmers during a 14-day altitude training camp (ATC) at 1500 m. The study subjects were five Olympic-level open-water swimmers (mean age: 25.0 ± 3.2 years; 3 females and 2 males). All subjects wore a wrist activity monitor and filled a sleep diary for 18 consecutive nights, 4 nights before and 14 nights during ATC. The data were then analyzed at four different time points: before ATC (PRE), the first two days of ATC (T1), and after one (T2) and two weeks of ATC (T3). Training load, assessed as the covered distance (km), session rating of perceived exertion (sRPE), and heart rate (HR), was monitored during the week before and the first and second week of ATC. No significant differences in objective and subjective scores of sleep quality were detected, whereas the sleep onset time (p = 0.018; η2p = 0.83, large) and sleep offset time (p < 0.001; η2p = 0.95, large) significantly differed among PRE, T1, T2, and T3: elite athletes started to sleep and woke up ≃ 1 h earlier the first two days of ATC compared to PRE (sleep onset time: p = 0.049; sleep offset time: p = 0.016). Further, an increase in the training volume during the two weeks of the ATC was observed, with the most time spent in a low-intensity regime and an increase in time spent in a high-intensity regime compared to PRE. Sleep quality was not negatively influenced by a 14-day altitude training camp at 1500 m in a group of Olympic-level elite swimmers despite an increase in perceived exertion during training sessions. Nonetheless, early sleep onset and sleep offset times were observed for the first two nights of ATC: elite athletes started to sleep and woke up ≃ 1 h earlier compared to the baseline nights.

Carbohydrate supplementation during a simulated 10-km open water swimming race: effects on physiological, perceptual parameters and performance.

The aim of the present study was to test the effect of carbohydrate ingestion, simulating a 10-km open water race competition on energy cost (Csw), perceived exertion (RPE), heart rate (HR), stroke rate (SR) and performance. We hypothesized that carbohydrate ingestion would reduce Csw and RPE in elite open water swimmers (OW-swimmers) and improve performance. Eight elite OW-swimmers swam for 3 × 30 min with 20-s of interval necessary to collect data in the swimming flume at a pre-set pace corresponding to their 10-km race pace, followed by a time to exhaustion test (TTE) at 100% of the peak oxygen uptake (V̇O2peak). During the set, OW-swimmers ingested 45-g of carbohydrates (CHO) in 550-mL of water (8% solution) during each of the two intervals or a placebo solution (PLA). HR, RPE, V̇O2 and SR were measured. Shapiro-Wilk test was used to verify the normal distribution of data. Two-way repeated measures ANOVA and t-test was performed (p < 0.05). A significant difference emerged in TTE between the trials (169.00 ± 91.06 s in CHO; 102.31 ± 57.47 s in PLA). HR, RPE and SR increased during the TTE but did not differ between trials. Csw did not show a significant main effect between the two conditions and in time course in both conditions. CHO ingestion significantly increased TTE at 100% of V̇O2peak after 90-min of swimming at 10-km race pace. These findings indicate that CHO intake during a 10-km open water swimming competition should have a beneficial impact on performance in the final part of the race.

Monitoring Rating of Perceived Exertion Time in Zone: A Novel Method to Quantify Training Load in Elite Open-Water Swimmers?

PURPOSE: To analyze training-intensity distribution (TID) using different independent monitoring systems for internal training load in a group of elite open-water swimmers. METHODS: One hundred sixty training sessions were monitored in 4 elite open-water swimmers (2 females and 2 males: 23.75 [4.86] y, 62.25 [6.18] kg, 167 [6.68] cm) during 5 weeks of regular training. Heart-rate-based methods, such as time in zone (TIZ), session goal (SG), and hybrid (SG/TIZ), were used to analyze TID. Similarly to SG/TIZ, a new hybrid approach, the rating of perceived exertion (RPE)/TIZ for a more accurate analysis of TID was used. Moreover, based on the 3-zone model, the session ratings of perceived exertion of the swimmers and the coach were compared. RESULTS: Heart-rate- and RPE-based TID methods were significantly different in quantifying Z1 (P = .012; effect size [ES] = 0.490) and Z2 (P = .006; ES = 0.778), while no difference was observed in the quantification of Z3 (P = .428; ES = 0.223). The heart-rate-based data for Z1, Z2, and Z3 were 83.2%, 7.4%, and 8.1% for TIZ; 80.8%, 8.3%, and 10.8% for SG/TIZ; and 55%, 15.6%, and 29.4% for SG. The RPE-based data were 70.9%, 19.9%, and 9.2% for RPE/TIZ% and 41.2%, 48.9%, and 9.7% for the session rating of perceived exertion. No differences were observed between the coach's and the swimmers' session ratings of perceived exertion in the 3 zones (Z1: P = .663, ES = -0.187; Z2: P = .110, ES = 0.578; Z3: P = .149, ES = 0.420). CONCLUSION: Using RPE-based TID methods, Z2 was significantly larger compared with Z1. These results show that RPE-based TID methods in elite open-water swimmers are affected by both intensity and volume.

Pacing and Hazard Score of Elite Open Water Swimmers During a 5-km Indoor Pool Race.

PURPOSE: The sensation of fatigue experienced at a certain point of the race is an important factor in the regulation of pacing. The rating of perceived exertion (RPE) is considered one of the main mediators utilized by athletes to modify pacing. The aim was to analyze the relationship between pacing and RPE of elite open water swimmers during national indoor pool championships. METHODS: A total of 17 elite open water swimmers (males, n = 9; females, n = 8) agreed to provide RPE every 500 m during the finals of the national championships 5-km indoor pool race. Time splits, stroke rate, and RPE were collected every 500 m. The Hazard score was calculated by multiplying the momentary RPE by the remaining fraction of the race. Athletes were placed in one of two categories: medalists or nonmedalists. For all variables, separate mixed analysis of variances (P ≤ .05) with repeated measures were used considering the splits (ie, every 500 m) as within-subjects factor and the groups (ie, medalists and nonmedalists) as between-subjects factor. RESULTS: Average swimming speed showed a significant main effect for split for both males and females (P < .001). A significant interaction was observed between average swimming speed and groups for females (P = .032). RPE increased in both groups (P < .001) with no difference observed between groups. However, the female nonmedalists showed a disproportionate nonlinear increase in RPE (5.20 [2.31]) halfway through the event that corresponded to the point where they started significantly decreasing speed. CONCLUSIONS: The results of the present study show different pacing strategies adopted by medalists and nonmedalists despite a similar RPE.

Session RPE Breakpoints Corresponding to Intensity Thresholds in Elite Open Water Swimmers.

This study aims to assess the correspondence between session rating of perceived exertion (sRPE) breakpoints with both the first lactate threshold (LT1) and the second lactate threshold (LT2) in elite open water swimmers (OWS). Six elite OWS of the National Olympic Team specialized in distances between 5 and 25 km participated to the study. OWS performed a set of 6 times 500 m incremental swimming step test during which blood lactate concentration (BLC), split time (ST), stroke frequency (SF), and rating of perceived exertion (RPE) were collected. To assess the corresponding breakpoints, we considered LT1 as the highest workload not associated with rise in BLC and LT2 as the increase of 2mM above LT1. According to the LT1 and LT2, the identified zones were: Z1 ≤3, Z2 between 4 and 6, Z3 ≥ 7. In conclusion, the intensity zones determined for OWS resulted different from what previously reported for other endurance disciplines.