ABSTRACT
Colistin resistance genes mcr-3 and mcr-1 have been detected in an Escherichia coli isolate from cattle faeces in a Spanish slaughterhouse in 2015. The sequences of both genes hybridised to same plasmid band of ca 250 kb, although colistin resistance was non-mobilisable. The isolate was producing extended-spectrum beta-lactamases and belonged to serotype O9:H10 and sequence type ST533. Here we report an mcr-3 gene detected in Europe following earlier reports from Asia and the United States.
Subject(s)
Anti-Bacterial Agents/pharmacology , Cattle/microbiology , Colistin/pharmacology , Escherichia coli Proteins/genetics , Escherichia coli/drug effects , Escherichia coli/genetics , beta-Lactamases/genetics , Animals , Anti-Bacterial Agents/administration & dosage , Colistin/administration & dosage , Drug Resistance, Bacterial/genetics , Escherichia coli/isolation & purification , Escherichia coli Infections/microbiology , Escherichia coli Infections/veterinary , Feces/microbiology , Microbial Sensitivity Tests , Molecular Typing , Peptides , Polymerase Chain Reaction , Sentinel SurveillanceSubject(s)
Drug Resistance, Bacterial/genetics , Escherichia coli Proteins/genetics , Escherichia coli/genetics , beta-Lactamases/genetics , Aged , Anti-Bacterial Agents/therapeutic use , Drug Resistance, Bacterial/drug effects , Escherichia coli/drug effects , Escherichia coli/isolation & purification , Escherichia coli Infections/drug therapy , Escherichia coli Infections/microbiology , Humans , Male , Plasmids , Respiratory Tract Infections/drug therapy , Respiratory Tract Infections/microbiology , Spain , Treatment FailureABSTRACT
Recent findings suggest that use of colistin as a last resort antibiotic is seriously threatened by the rise of a new plasmid mediated mechanism of resistance (MCR-1). This work identifies, for the first time in Southern Europe, the gene mcr-1 in nine strains from farm animals (poultry and swine) corresponding to five Escherichia coli and four Salmonella enterica, among which three belong to serovar Typhimurium and one to Rissen. The MCR-1 was found encoded by a plasmid highly mobilizable by conjugation to the E. coli J53 strain. Two E. coli strains carried two determinants, mcr-1 plus pmrA or pmrB mutations, known to confer colistin resistance.
