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Water Res ; 40(6): 1217-23, 2006 Mar.
Article in English | MEDLINE | ID: mdl-16516265

ABSTRACT

An oxadiazon-degrading bacterial, Pseudomonas strain CG5, was isolated from an agricultural contaminated soil. This strain CG5 was able to grow on 10mg of oxadiazon per l, yielding 5.18+/-0.2 mg of protein biomass mol(-1). GC-MS analyses of the metabolites from oxadiazon catabolism revealed its dehalogenation and degradation to form non-toxic end-products, cells were then immobilized by adsorption on a ceramic support to be used as biocatalysts in herbicide removal biofilm-reactor processes. Seventy-two per cent of the oxadiazon was removed, and the maximum specific substrate uptake rate was 10.63+/-0.5 microg h(-1) mg(-1) prot. A new mathematical model was developed to interpret and predict the behaviour of the bacteria and pollutants in a biofilm-reactor system, to consider biofilm structural and morphological properties.


Subject(s)
Herbicides/metabolism , Oxadiazoles/metabolism , Pseudomonas fluorescens/metabolism , Soil Microbiology , Soil Pollutants/metabolism , Biodegradation, Environmental , Bioreactors , Microscopy, Confocal , Models, Theoretical , Pilot Projects
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