Hyperspectral imaging for small-scale analysis of Hordeum vulgare L. leaves under the benzo[a]pyrene effect.

Hyperspectral imaging is a newly developed approach to estimate the current state of the plants and to develop the methods of soil and plant ecological state improvement under the effect of different sources. The study was devoted to the novel approach of hyperspectral imaging application in the case of persistent organic pollutants (POP) uptake by plants. Hordeum vulgare L. was used as a test plant and grown on the soil artificially contaminated by benzo[a]pyrene (BaP) in the doses of 20, 100, 200, 400, and 800 ng g-1, which corresponds to 1, 5, 10, 20, and 40 maximum permissible concentrations (MPC) and correlates with the level of soil pollution near industrial facilities in the Rostov Region (Russian Federation). It was analyzed a group of indexes responsible for plants stress, consists of broadband greenness group, narrowband greenness group, light use efficiency group, and leaf pigments group. Benzo[a]pyrene had a stronger effect on the efficiency of the photosynthesis process than on the content of chlorophylls. In the phase of active adaptation to stress in H. vulgare, the content of photosynthetic pigments was increased. The proposed method for selecting spectral profiles by cutting off profiles that do not belong to a plant, based on the NDVI value can be effectively used for the estimation of the plants stress under the BaP contamination and for future perspectives in the most suitable way for the application of the plant's growth stimulants.

IL-6 Activities in the Tumour Microenvironment. Part 1.

The predominant role of IL-6 in cancer is its key promotion of tumour growth. IL-6 binds IL-6 receptor (IL-6R) and the membrane-bound glycoprotein gp130. The complex I-6/IL-6R/gp130 starts the Janus kinases (JAKs) and signal transducer and activator of transcription 3 (STAT3) or JAK/STAT3 pathway. IL-6 R exits in two forms: a membrane-bound IL-6Rα subunit (mIL-6R) that participates in classic signalling pathway and soluble IL-6R subunit (sIL-6R) engaged in trans-signalling. The pro-tumour functions of IL-6 are associated with STAT3, a major oncogenic transcription factor that triggers up-regulation of target genes responsible for tumour cell survival. IL-6 combined with TGF-ß induces proliferation of pathogenic Th17 cells. The anti-tumour function of IL-6 is the promotion of anti-tumour immunity. IL-6 trans-signaling contributed to transmigration of lymphocytes in high endothelial venules (HEV). Dendritic cell (DC) secreted IL-6 in the lymph node influences the activation, distribution and polarisation of the immune response. Elevated serum levels of IL-6 and increased expression of IL-6 in tumour tissue are negative prognostic marker for patients' survival.

Immunohistochemical Expression of TGF-Β1, SMAD4, SMAD7, TGFßRII and CD68-Positive TAM Densities in Papillary Thyroid Cancer.

BACKGROUND: Papillary thyroid carcinoma (PTC) accounts for 80% of the thyroid malignancies that are characterised by slow growth and an excellent prognosis. Over-expression of SMAD4 protein restores TGF-ß signalling, determines a strong increase in anti-proliferative effect and reduces invasive potential of tumour cells expressing it. AIM: The study aimed to analyse the immunohistochemical expression of TGF-ß1 and its downstream phosphorylated SMAD4, element and of the inhibitory SMAD7 PTC variants and their association with the localisation of TAMs within the tumour microenvironment. METHODS: For this retrospective study we investigated 69 patients immunohistochemistry with antibodies against TGF-ß, TGF - ß-RII, SMAD4, SMAD7, CD68+ macrophages. RESULTS: Patients with low infiltration with CD68+ cells in tumour stroma has significantly shorter survival (median of 129.267 months) compared to those with high CD68+ cells infiltration (p = 0.034). From the analysis of CD68+ cells in tumour border and tumour stroma correlated with expression of TGF-ß1 / SMAD proteins, we observed that the positive expression of TGF-ß1 in tumour cytoplasm, significantly correlated with increased number of CD68+ cells in tumour border (X2 = 5,945; p = 0.015). CONCLUSION: TGF-ß enhances motility and stimulates recruitment of monocytes, macrophages and other immune cells while directly inhibiting their anti-tumour effector functions.

Expression of E-Cadherin/Beta-Catenin in Epithelial Carcinomas of the Thyroid Gland.

BACKGROUND: The aberrant activation of Wnt signalling pathway may be a common denominator for the development of thyroid tumorigenesis. It was announced that the loss of E-cadherin rather than ß-catenin mutation represents a crucial event in determining the degree of differentiation of thyroid carcinomas. AIM: The aim of the study was to evaluate the expression of E-cadherin and ß-catenin in the thyroid cancer tissue and to correlate these data with some histological and clinical parameters of the tumours. MATERIAL AND METHODS: We investigated 112 patients, having thyroid tumours - papillary, follicular, anaplastic and oncocytic carcinomas immunohistochemically with antibodies against E-cadherin and ß-catenin. Survival analyses were done. RESULTS: E-cadherin expression was focally retained in the tumour cell membranes and the tumour cell cytoplasm of the papillary, follicular and oncocytic thyroid cancers, weather in anaplastic cancers it was almost lost (p = 0.0042, and p = 0.019, respectively, Fisher's Exact Test). The expression of ß-catenin in tumour cytoplasm and membrane in papillary cancers was higher as compared to that in the other tumours (p = 0.111, and p = 0.0104, respectively). CONCLUSION: Not surprisingly, the presence of aberrant expression of E-cadherin and ß-catenin in thyroid cancer has been associated with better patients' prognosis and better differentiated tumour histology.

Two-dimensional fluorescence difference gel electrophoresis analysis of Listeria monocytogenes submitted to a redox shock.

The influence of redox alteration on the growth and proteomic pattern of Listeria monocytogenes was investigated. A redox shock was induced in cultures by addition of 3mM ferricyanide (FeCN) and 6mM dithiothreitol (DTT) to increase or to decrease respectively the redox potential naturally occurring at the beginning of growth. In both conditions, the reducing and oxidizing redox shock had a strong influence, decreasing the maximum growth rate by half compared to a control culture. The proteomic analysis of L. monocytogenes performed by two-dimensional difference gel electrophoresis (2D-DIGE) exhibited twenty-three proteins differentially expressed (P<0.05), among these, many were oxidoreductases, and proteins involved in cellular metabolism (glycolysis, protein synthesis), detoxification (kat) or adhesion (Lmo1634).