Subject(s)
Culture Media/metabolism , DNA/biosynthesis , Nucleic Acid Precursors/metabolism , Animals , Cell Line , Chick Embryo , Humans , Thymidine/metabolism , Time Factors , Tritium , Virus CultivationSubject(s)
Polyethylene Glycols/pharmacology , Animals , Drug Interactions , Humans , Hydrogen-Ion Concentration , Immunologic Techniques , Indicators and Reagents/pharmacology , Macromolecular Substances , Molecular Weight , Polyethylene Glycols/analysis , Polyethylene Glycols/therapeutic use , Structure-Activity Relationship , Technology, PharmaceuticalABSTRACT
A relationship has been studied between the growth-stimulating activity and chemical composition of bovine serum (BS) that is widely used for cell culture growth and virus replication. A direct correlation was shown between the growth-promoting activity of bovine serum, manifested on the model of chick embryo fibroblasts, and the contents of phospholipids, total lipids, proteins, and calcium in this serum. A significant inverse association has also been revealed between the growth-promoting activity of the serum and the amounts of cholesterol and glucose in this.
Subject(s)
Cells, Cultured/drug effects , Immune Sera/pharmacology , Animals , Cattle , Chemical Phenomena , Chemistry , Chick Embryo , Culture Media/pharmacology , Fibroblasts/drug effects , Stimulation, Chemical , Structure-Activity RelationshipSubject(s)
Cells, Cultured , Culture Media/standards , Plasma , Polyethylene Glycols , Animals , CattleABSTRACT
The authors describe a method of obtaining monospecific serum against secretory IgA and the corresponding standard. An immunochemically pure (11.6S) secretory human IgA was extracted from the colostrum by salt fractionation and gel-filtration through Sephadex G-200 and Sepharose 6B; this IgA was used as an antigen for the immunization and the standard for the quantitative determination of SIgA in the secretions. Monospecific anti-SC-serum was obtained by successive exhaustion of the antiserum against the S IgA immunosorbents prepared from normal human serum and the serum of a patient suffering from A myeloma containing polymeric IgA forms.