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3.
Tsitologiia ; 25(10): 1216-8, 1983 Oct.
Article in Russian | MEDLINE | ID: mdl-6659066

ABSTRACT

A relationship has been studied between the growth-stimulating activity and chemical composition of bovine serum (BS) that is widely used for cell culture growth and virus replication. A direct correlation was shown between the growth-promoting activity of bovine serum, manifested on the model of chick embryo fibroblasts, and the contents of phospholipids, total lipids, proteins, and calcium in this serum. A significant inverse association has also been revealed between the growth-promoting activity of the serum and the amounts of cholesterol and glucose in this.


Subject(s)
Cells, Cultured/drug effects , Immune Sera/pharmacology , Animals , Cattle , Chemical Phenomena , Chemistry , Chick Embryo , Culture Media/pharmacology , Fibroblasts/drug effects , Stimulation, Chemical , Structure-Activity Relationship
5.
Article in Russian | MEDLINE | ID: mdl-1087797

ABSTRACT

The authors describe a method of obtaining monospecific serum against secretory IgA and the corresponding standard. An immunochemically pure (11.6S) secretory human IgA was extracted from the colostrum by salt fractionation and gel-filtration through Sephadex G-200 and Sepharose 6B; this IgA was used as an antigen for the immunization and the standard for the quantitative determination of SIgA in the secretions. Monospecific anti-SC-serum was obtained by successive exhaustion of the antiserum against the S IgA immunosorbents prepared from normal human serum and the serum of a patient suffering from A myeloma containing polymeric IgA forms.


Subject(s)
Antibodies, Anti-Idiotypic , Immunoglobulin A , Antibodies, Anti-Idiotypic/isolation & purification , Chromatography, Gel , Colostrum/immunology , Female , Humans , Immunoelectrophoresis , Immunoglobulin A/isolation & purification , Pregnancy
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