ABSTRACT
The authors present experience accumulated by "RADON" Industrial Research Association in treating liquid radioactive waste. According to the presentation, activities of "R ADON" Industrial Research Association develop in three directions--evolving technical means to purify radioactive waters in "RADON" Industrial Research Association, advancing mobile plants to purify radioactive waters in other institutions, elaborating new technologies for liquid radioactive waste purifications within numerous national and international projects and agreements with various organizations (including those associated with nuclear power stations and nuclear submarines).
Subject(s)
Radioactive Waste , Waste Disposal, Fluid , Water Pollutants, Radioactive , Water Pollution, Radioactive , Water Purification , Humans , Nuclear Energy , Power Plants , Ships , Water Pollutants, Radioactive/analysis , Water Pollution, Radioactive/analysisABSTRACT
Nonpolar regions (hydrophobic nuclei and microclusters) in porcine pancreatic phospholipase A2 have been investigated and described. Based on the atomic coordinates, we analyzed all nonpolar contacts for all nonpolar groups of each amino acid residue and identified three hydrophobic nuclei (gamma 1, gamma 2, gamma 3) and four microclusters (mu 4, mu 5, mu 6, mu 7) in the enzyme. These nonpolar regions include hydrophobic residues as well as residues with charged or polar side chain. Quantitative characteristics of hydrophobic nuclei show that the latter are condensations of nonpolar contacts in the protein. The stability of the nuclei is comparable to that of the secondary structure elements. Comparative analysis of nonpolar areas in native porcine pancreatic phospholipase A2, mutant enzyme, and its complex with a substrate analog demonstrated the role of nonpolar contacts in protein structure and function.
Subject(s)
Phospholipases A/chemistry , Amino Acids/analysis , Animals , Mutation , Pancreas/enzymology , Phospholipases A2 , Protein Conformation , SwineSubject(s)
Adaptation, Physiological , Altitude , Personality Development , Psychology, Adolescent , Somatotypes , Adolescent , HumansABSTRACT
A detailed analysis of noncovalent interactions in the RNase A molecule was carried out. For this purpose, contact maps based on structural data were constructed. Three hydrophobic nuclei and five microclusters were identified, and their quantitative parameters were calculated. The contacts between amino acid residues of the active center and the hydrophobic nuclei were established. The distribution of the charged amino acid residues on the of RNase A surface was shown. The data obtained was discussed in the context of the ribonuclease A family. The substitutions seen from the alignment of amino acid sequences in the family might have influence on the spatial structure of different parts of RNase A and their mutual orientation and, consequently on the biological activity of family members.
Subject(s)
Proteins/chemistry , Ribonuclease, Pancreatic/chemistry , Amino Acid Sequence , Animals , Humans , Molecular Sequence Data , Sequence Alignment , Structure-Activity RelationshipABSTRACT
We present the application of a new method for analysis of nonpolar structure of proteins. A detailed analysis of the composition and properties of nonpolar nuclei and microclusters of microbial ribonucleases with known sequence have been carried out on the basis of 3D-structure of RNase Pbi and that of RNase Ti. It has been shown that all residues in nonpolar nuclei have high homology, about 95% for proteins with an identical scheme of S-S bridges and about 75% for nonidentical scheme of S-S bridges. The stability of nonpolar nuclei, conservation of their composition and their position in the protein globule allows one to assume that they play an important functional role in protein structure and possibly can be considered as independent structural elements of 3D-structure of a protein.
Subject(s)
Fungi/enzymology , Ribonucleases/chemistry , Amino Acid Sequence , Molecular Sequence Data , Protein ConformationSubject(s)
Encephalitis, Tick-Borne/diagnosis , Adolescent , Adult , Body Temperature , Encephalitis, Tick-Borne/immunology , Humans , Kyrgyzstan , MaleSubject(s)
Child Health Services , Vaccination , Child , Child, Preschool , Humans , Infant , KyrgyzstanABSTRACT
Strains of the causative agent of tularemia were for the first time isolated by the authors from the objects of the external environment in the tundra zone of Eastern Taimir; this indicates a possibility of preservation of the microbe under conditions of the extreme north.
Subject(s)
Francisella tularensis , Tularemia/epidemiology , Animals , Francisella tularensis/isolation & purification , Mice , SiberiaABSTRACT
The paper is a summary of the therapeutical effectiveness of rhythmotherapeutical sleep in some neuropsychic and neurosomatic disorders. During 8 years of approbation of this method 740 patients (adults and children) received such treatment. The positive therapeutical effect seen in more than than 80% of the cases permits to highly estimate this method and the apparatus (LIDA) used for these purposes.
Subject(s)
Brain Diseases/therapy , Electronarcosis , Mental Disorders/therapy , Sleep Wake Disorders/therapy , Adolescent , Adult , Aged , Child , Child, Preschool , Electronarcosis/methods , Humans , Middle AgedABSTRACT
The authors present the results of bacteriological and serological study for tularemia of 498 lemmings caught in Taimyr. Positive results were revealed in 4 out of 98 sera examined in the indirect hemagglutination test. In carrying out 67 biological tests on albino mice there were isolated for the first time in the Soviet Union 6 cultures of the causative agent of tularemia from the spleen of lemmings. By morphological, cultural and virulent properties the cultures obtained failed to differ from those isolated in other regions of the Soviet Union, and, consequently, we referred to the holoarctic race. Thus, it was established by the authors (both serologically and bacteriologically) that there existed tundra foci of tularemia.
