Effect of feeding BHC on the biochemical parameters of blood in rabbits.

Studies were made by feeding 25 mg per kg body weight of BHC to rabbits daily for twelve weeks. BHC in the blood increased progressively without affecting blood pH. The levels of glucose, cholesterol, total proteins and creatinine increased while that of urea decreased in the blood of treated animals. The differences among the control and treated rabbits for these parameters were highly significant as every one microgram per ml accumulation of BHC in the blood, affected a corresponding increase of 1.82 mg/dl glucose, 2.618 mg/dl of cholesterol, 0.02 g/dl of total proteins and 0.13 mg/dl of creatinine, while a decrease of 0.105 mg/dl of urea was observed. The feeding of BHC, therefore, affected these vital biochemical parameters significantly.

Disposition kinetics of sulphadiazine in normal and diabetic rabbits.

Influence of alloxan induced metabolic disorder on the biodisposition kinetics of a weakly acidic drug sulphadiazine was investigated in rabbits. In 12 rabbits, mean +/- SD blood pH was 7.45 +/- 0.05, plasma glucose 123 +/- 10.6 mg%, plasma total lipids 336 +/- 124 mg% and plasma urea 36 +/- 6 mg%. After treatment with 150 mg/kg alloxan intravenously the blood pH was 7.20 +/- 0.06, plasma glucose 393 +/- 80.3, total lipids 532 +/- 181 and plasma urea was 48 +/- 10 mg%. Disposition kinetics showed that the plasma concentration of sulphadiazine was significantly (P less than 0.01) higher in normal condition when compared with that of metabolically altered. The zero-time plasma concentration was significantly (P less than 0.01) higher, apparent volume of distribution and total body clearance were significantly (P less than 0.01) lower in the normal than in the metabolically altered condition of rabbits. Elimination rate constant and the half-life did not reveal any significant difference in the normal and metabolically altered conditions. These studies demonstrate that the clinical biochemical conditions can influence the disposition kinetics and fate of weakly acid, and possibly of the weakly basic drugs. Therefore, the therapeutic standards of the drugs need be verified in the condition in which the drugs are to be employed clinically.

Preliminary screening of amino acids excretion in the urine of newly born babies.

The paper attempts to characterize the qualitative as well as quantitative estimations of aminoacids in the urines of new born babies using two dimensional paper chromatography. The objective of this investigation was to know the extent of amino acid defects in these samples and to correlate such defects with the physical abnormalities in infants. Based on the overall results, it is inferred that phenylalanine excreted in the urine gave a positive response with PKU test and some particular amino acids are identified and estimated in the urine of new born babies to confirm the diagnosis of phenylketonuric patients.