ABSTRACT
We analyzed a collection of carbapenem-resistant Gram-negative bacterial isolates and detected VIM-1, VIM-2, and KPC-2 in diverse enterobacterial species and Pseudomonas aeruginosa isolates. Our findings suggest a more widespread dissemination of carbapenemases in Germany than currently appreciated.
Subject(s)
Anti-Bacterial Agents/pharmacology , Bacterial Proteins/genetics , Carbapenems/pharmacology , Gram-Negative Bacteria/drug effects , beta-Lactamases/genetics , Drug Resistance, Multiple, Bacterial/genetics , Germany , Gram-Negative Bacteria/genetics , Gram-Negative Bacteria/isolation & purification , Humans , Microbial Sensitivity TestsABSTRACT
BACKGROUND: Influenza A (H1N1) has emerged as a considerable threat for recipients of organ transplants. Vaccination against the novel influenza A (H1N1) virus has generally been advocated. There is limited experience with AS03-adjuvanted A/H1N1 pandemic influenza vaccines in immunosuppressed patients. METHODS: We conducted an observational, nonrandomized single-center study to assess antibody response and vaccine-related adverse effects in 47 heart transplant recipients (44 men; age, 56±13 years). The AS03-adjuvanted, inactivated split-virion A/California/7/2009 H1N1v pandemic vaccine was administered. Antibody titers were measured using hemagglutination inhibition; immunoglobulin G (IgG) response was assessed using a new pandemic influenza A IgG enzyme-linked immunosorbent assay (ELISA) test kit and compared with hemagglutination-inhibition titers. Adverse effects of vaccination were assessed by a questionnaire. RESULTS: Postvaccination antibody titers of greater than or equal to 1:40 were found in only 15 patients, corresponding to a seroprotection rate of 32% (95% confidence interval, 19%-47%). Sensitivity, specificity, positive predictive value, and negative predictive value of ELISA testing were 80.0%, 68.8%, 54.5%, and 88.0%, respectively. Age, time posttransplantation, and immunosuppressive regimen did not impact antibody response. Vaccination was well tolerated. CONCLUSIONS: Single-dose administration of an AS03-adjuvanted vaccine against the novel influenza A (H1N1) virus did not elicit seroprotective antibody concentrations in a substantial proportion of heart transplant recipients; the new pandemic influenza A IgG ELISA test kit proved to be of limited clinical use.
Subject(s)
Adjuvants, Immunologic/administration & dosage , Antibodies, Viral/blood , Heart Transplantation/immunology , Influenza A Virus, H1N1 Subtype/immunology , Influenza Vaccines/administration & dosage , Adult , Aged , Enzyme-Linked Immunosorbent Assay , Female , Hemagglutination Inhibition Tests , Humans , Immunoglobulin G/blood , Influenza Vaccines/adverse effects , Influenza, Human/epidemiology , Influenza, Human/immunology , Influenza, Human/prevention & control , Male , Middle Aged , Pandemics/prevention & controlABSTRACT
The spectrum of manifestations and management of the novel influenza A/H1N1 virus in transplanted patients is currently of major concern. Asymptomatic infections are less common yet important for spreading of the virus and thus affect containment measures. To our knowledge, there are no reports of asymptomatic infections with influenza A/H1N1 in immunosuppressed patients. We present the first case of a young heart transplant recipient who remained asymptomatic despite positive polymerase chain reaction (PCR) after exposure to individuals with influenza A/H1N1.
Subject(s)
Heart Transplantation , Influenza A Virus, H1N1 Subtype , Influenza, Human/diagnosis , Opportunistic Infections/diagnosis , Adult , Graft Rejection/prevention & control , Humans , Immunosuppressive Agents/administration & dosage , Immunosuppressive Agents/adverse effects , Influenza A Virus, H1N1 Subtype/genetics , Male , Myocardial Ischemia/surgery , Polymerase Chain Reaction , Risk FactorsABSTRACT
BKV infection is a potential cause of renal dysfunction in non-renal organ transplant recipients. JCV is the causative agent of PML. Furthermore, polyomaviruses are tumor inducing viruses and molecular data suggest an association with malignancies among solid organ transplant patients. So far, there are no studies analyzing polyomavirus viruria following Ltx in children. We performed a prospective prevalence study at a mean of 2187 (range 20-5671) days after transplantation in 100 consecutive children admitted for the routine follow-up examination post-Ltx. The urine was screened for BKV and JCV DNA by using PCR in each case. A plasma analysis by PCR was also done if more than 100,000 DNA copies/mL urine were detected. BKV or JCV viruria was found in 19% (n = 19) of our patients. All patients were free of clinical signs of viral infection, PML, or nephropathy. GFR was normal in 97% of patients and we found no statistical difference of kidney function between patients with and without BKV/JCV viruria. The extent of immunosuppressive therapy had no influence on the polyomavirus viruria. Overall, we found a higher prevalence of polyomavirus viruria in our pediatric liver transplant recipients than reported in adult patients.
Subject(s)
BK Virus/isolation & purification , DNA, Viral/urine , JC Virus/isolation & purification , Liver Transplantation/adverse effects , Polyomavirus Infections/epidemiology , Tumor Virus Infections/epidemiology , Urine/virology , Adolescent , BK Virus/genetics , Child , Child, Preschool , Female , Follow-Up Studies , Germany/epidemiology , Humans , Infant , JC Virus/genetics , Male , Polymerase Chain Reaction , Polyomavirus/genetics , Polyomavirus/isolation & purification , Polyomavirus Infections/urine , Polyomavirus Infections/virology , Prevalence , Prospective Studies , Time Factors , Tumor Virus Infections/urine , Tumor Virus Infections/virology , Young AdultABSTRACT
BACKGROUND: Infections caused by Nocardia farcinica are uncommon and show a great variety of clinical manifestations in immunocompetent and immunocompromised patients. Because of its unspecific symptoms and tendency to disseminate it may mimic the clinical symptoms and radiologic findings of a tumour disease and the diagnosis of nocardiosis can easily be missed, because there are no characteristic symptoms. CASE PRESENTATION: We present a case of an adrenal gland abscess caused by subacute disseminated N. farcinica pneumonia. CONCLUSION: An infection with N. farcinica is potentially lethal because of its tendency to disseminate -particularly in the brain- and its high resistance to antibiotics. Awareness of this differential diagnosis allows early and appropriate treatment to be administered.
