Dental and dentofacial problems in a female child with Toriello-Carey -syndrome: changes in 3 years.

Toriello-Carey syndrome is a rare disease whose clinical manifestations are midline facial defects, laryngeal and pharyngeal hypoplasia, cardiac defect, and corpus callosum hypoplasia. Literature states that clinical manifestations are more evident in males. This is the second report in the literature which describes the dental and dentofacial -features in an 8-year-old female patient with Toriello-Carey syndrome.

Mineral trioxide aggregate as a pulpotomy agent in immature teeth: Long-term case report.

This case report aimed to present the long-term clinical performance of mineral trioxide aggregate (MTA) pulpotomies in immature permanent teeth. Four patients with complicated crown fractures of five maxillary immature central incisor teeth were treated with pulpotomy using gray MTA. An immature mandibular first molar showing signs of reversible pulpitis that was exposed mechanically during cavity preparation was also treated with MTA pulpotomy. Four of the 6 immature teeth were diagnosed vitally with complete root maturation and with the presence of dentin bridges after 55 months (mean time of follow-ups). MTA pulpotomy was assessed unsuccessfully in two fractured central incisors. Severe discoloration in the crowns was present in all 6 cases. MTA may induce pulp healing with dentin bridge formation and prevent necrosis at long-term periods in most of the pulpotomy cases. However, discoloration following MTA pulpotomy is a significant clinical complication.

Laryngeal dysfunction and oral hygiene: Any relation?

OBJECTIVE: The purpose of this study is to establish a relation between poor oral hygiene and laryngeal dysfunction. METHODS: 43 adult patients were divided into two groups according to caries activity and oral hygiene. 18 patients with oral hygiene index score (OHI-S) 0-1 were grouped as the control group (good oral hygiene). 25 patients with OHI-S 2-3 were grouped as the study group (poor oral hygiene). Larygostroboscopic examination, aerodynamic measures by defining maximum phonation time (MPT) and s/z ratio and the pitch level measurements were done. Patients with gastroesophageal reflux disease (GERD), laryngopharyngeal reflux (LPR), previously confirmed laryngeal diseases or pathologies, systemic other diseases, or smokers were excluded from this study. RESULTS: The average MPT values of the study group were found to be statistically very significantly lower than those of the control group (p<0.01). In the stroboscopic findings, the proportion of normal closure levels were meaningfully higher in the control group than in the study group (p<0.05). The supraglottic involvement was found statistically meaningfully higher in the study group than in the control group (p<0.05). The proportion of normal closure phase incidences were meaningfully higher in the control group than in the study group (p<0.05). CONCLUSION: So, our findings of high glottic closure impairment, supraglottic involvement and low MPT scores in the poor oral hygiene group correlate with LPR findings such as muscle tension dysphonia. Poor oral hygiene may aggravate potential LPR in people.

Success rates of mineral trioxide aggregate, ferric sulfate, and formocresol pulpotomies: a 24-month study.

PURPOSE: The purpose of this study was to evaluate the total success rates of mineral trioxide aggregate (MTA), ferric sulfate (FS), and formocresol (FC) as pulpotomy agents in primary molars. METHODS: A randomized, split-mouth study design was used in 32 healthy 5- to 7-year-old children with 128 carious primary molars without clinical or radiographic evidence of pulp degeneration. The pulpotomy agents were assigned as follows: Group 1=MTA; Group 2=FS; Group 3=1:5 diluted Buckley's FC; and Group 4=zinc oxide eugenol (ZOE) base. Clinical and radiographic follow-up at 6, 12, and 24 months used the following criteria: pain; swelling; sinus tract; mobility; internal root resorption; and furcation and/or periapical bone destruction. The data were analyzed using chi-square. RESULTS: No significant differences in success rates were found among the groups at 6 and 12 months. Success rates in groups 1 to 4 at 24 months were 96%, 88%, 88%, and 68% respectively. There was a significant difference (P<.001) between the MTA and ZOE groups at 24 months. CONCLUSIONS: ZOE, as the only pulpotomy medicament, had a significantly lower success rate than MTA. No significant differences were observed, among the 3 experimental materials (MTA, FC, and FS) at 2 years follow-up.

Cytotoxic effects of calcium hydroxide and mineral trioxide aggregate on 3T3 fibroblast cell line in vitro.

OBJECTIVES: To examine the cytotoxic effects of MTA and Ca(OH)2 on 3T3 fibroblasts at different time intervals. METHODS: Confluent cells were cultured with Ca(OH)2 and MTA in six-well plates. Wells with only fibroblasts served as controls. Cell number and viability were determined after 24 and 48 hours and 7 days of incubation. For cell viability, trypane blue exclusion assay was used. A Leitz inverted microscope was used to observe the morphological behavior of cells. The proliferation of cells was evaluated by BrdU assay. The results were analyzed with Dunn's multiple comparison, Friedman, and Kruskal-Wallis tests. RESULTS: No difference was seen in morphology of cells for either test material. Cells treated with MTA and Ca(OH)2 were reduced in number after 24 and 48 hours, respectively. A statistically significant difference was found in number of viable cells between test groups at 48 hours of incubation. The results of BrdU assay revealed low percentages of capable cells incorporating BrdU. CONCLUSIONS: The cytotoxic effects of the test materials-MTA and Ca(OH)2-on 3T3 cell line were evaluated as cytostatic for 24 and 48 hours, respectively. But this effect was reversible because the incubated cells showed normal cell proliferation at 48 hours and 7 days, respectively; MTA showed a significantly shorter cytotoxic effect on the cells.

Staphylococcus aureus transmission through oronasal fistula in children with cleft lip and palate.

OBJECTIVE: To determine the presence of Staphylococcus aureus in a nasal flora and oral environment, the correlation between frequency of transmission of S. aureus and oronasal fistula size, and the pattern of methicillin resistance on S. aureus strains in children with cleft lip and palate (CLP). DESIGN: Thirty-two CLP children with and without oronasal fistulas, ranging in age from 5 to 13 years were examined for oronasal fistula presence and size. Stimulated saliva samples and nasal swab samples were taken and investigated for S. aureus presence. S. aureus presence and counts were correlated with fistula presence and size. RESULTS: Saliva samples showed statistical differences between the groups with and without oronasal fistulas with an area ranging from 0.80 to 28.26 mm2. The S. aureus counts were significantly higher (r = .535, p = .002) in saliva samples from children with larger oronasal fistula. The S. aureus count was not significantly different (r = -.013, p = .942) in nasal samples compared with oronasal fistula size. Methicillin resistance with disk-diffusion method was recorded as sensitive (> or =13 mm) in all S. aureus strains. CONCLUSIONS: The results of this study indicate a positive correlation between fistula size and S. aureus transmission to one oral environment through oronasal fistulae, and a positive correlation between frequency of S. aureus transmission and fistula size. All S. aureus strains were sensitive to methicillin. These results may have implications for preventive treatment of CLP children.