ABSTRACT
Aim: To study the effect of antibiotic exometabolites of Bacillus amyloliquefaciens subsp. plantarum IMV B-7524 strain on the induction of defense responses in young plants of winter wheat. Methods: Microbiological, biochemical, phytopathological, statistical. Results: It was found that, when pre-watering winter wheat plants with salicylic acid, the cell-free culture supernatant and lipopeptide fraction of IMV B-7524 strain exometabolites during the further infection with phytopathogen Bipolaris sorokiniana, the damaged surface area of leaves decreased more than 10 times compared to the control. It was shown that in the roots and shoots of winter wheat plants L-phenylalanine ammonia-lyase (PAL) activity increased about 3 times in 2 hours after treatment. Conclusion: Reduction of the damaged surface area of leaves by the action of fungal phytopathogen and change of PAL activity in plants indicate the induction of defense responses under the influence of antibiotic exometabolites of B. amyloliquefaciens subsp. plantarum IMV B-7524 strain.
Subject(s)
Bacillus amyloliquefaciens/chemistry , Plant Diseases/microbiology , Triticum/physiology , Fungi/pathogenicity , Plant Leaves/microbiology , Plant Leaves/physiology , Triticum/microbiologyABSTRACT
Influence of Bacillus amyloliquefaciens subsp. plantarum IMV B-7404 strain exometabolites on phenylalanine ammonia-lyase (PAL, EC 4.3.1.24) activity in winter wheat seedlings has been studied. A significant increase of PAL activity at 4-6 hours after treatment of plant roots with Bacillus amyloliquefaciens subsp. plantarum IMVB-7404 strain exometabolites and in case of leaves infection with Bipolaris sorokiniana plant pathogen has been shown. It was established that PAL activity changed along with a decrease of the infected surface area of the leaves evidenced for the induction of response in winter wheat seedlings induced by IMV B-7404 strain exometabolites. It was concluded that the studied exponents could be used as model systems in the research of phytoimmunity induction mechanisms.
Subject(s)
Bacillus/metabolism , Biological Factors/pharmacology , Phenylalanine Ammonia-Lyase/metabolism , Plant Leaves/drug effects , Plant Proteins/metabolism , Seedlings/drug effects , Triticum/drug effects , Biological Factors/metabolism , Kinetics , Plant Diseases/immunology , Plant Immunity , Plant Leaves/enzymology , Plant Leaves/immunology , Saccharomycetales/growth & development , Saccharomycetales/pathogenicity , Seedlings/enzymology , Seedlings/immunology , Time Factors , Triticum/enzymology , Triticum/immunologyABSTRACT
The effect of pH value of cultivation medium on biosynthesis by bacteria of Bacillus genus of different types of hydrolases has been studied. The optimum pH medium values for each of the studied enzymes were chosen that allowed to increase the activity of produced xylanases and lypases--1.3-1.5 times, endoglucanases--4-5 times, cellobiases--18-20 times and pectinases more than 2 times.
Subject(s)
Bacillus/enzymology , Culture Media/chemistry , Hydrolases/biosynthesis , Bacillus/growth & development , Bacteriological Techniques , Hydrogen-Ion Concentration , Models, Biological , Temperature , Time FactorsABSTRACT
Influence of phytocompositions containing silica and medical herbs on growth and biological activity of probiotic strains of Bacillus subtilis and some representatives of obligate microflorae of intestine has been studied. Biocompatibility of studied strains with both pure aerosil bacteria, and its phytocompositions has been shown in the article. It is supposed that phytocompositions can be considered as components for creation of complex probiotic preparations from bacilli for the purpose to increase their therapeutic efficiency.
Subject(s)
Bacillus subtilis/drug effects , Bacillus subtilis/growth & development , Intestines/microbiology , Plant Extracts/pharmacology , Probiotics , Escherichia coli K12/drug effects , Escherichia coli K12/growth & development , Humans , Probiotics/standardsABSTRACT
Screening of aerobic sporulating bacteria of Bacillus genus from the collection of IMV ofNAS of Ukraine was performed to search for producers of cellulosolytic enzymes. Only 206 strains (58.4%) of 353 studied bacteria strains are capable to decompose carboxymethylcellulose, 158 strains (44.8%)--cellobiose. Under these conditions 91 strains (25% of all studied ones) synthesized actively both endoglucanase and cellobiase. It was shown that biosynthesis of enzymes of cellulose complex in bacilli was of inducible character, white the composition and activity of its components was a regulated process and was determined both by physiologic features of strains and by the conditions of their cultivation, composition of nutrient medium in particular. The strains of bacilli with high level of fermentation activity were selected as a result of screening.
Subject(s)
Bacillus/enzymology , Biotechnology/methods , Cellulase/metabolism , Cellulose/chemistry , Animal Feed , Bacillus/classification , Bacillus/growth & development , Hydrolysis , Substrate SpecificityABSTRACT
Research of influence of prebiotic lactitol on growth and biological activity of probiotic cultures Bacillus subtilis--the basis of the preparation endosporinum and some strains of the indigenous microflora of the human intestinal tract was conducted. Lactitol is actively utilized by bacteria B. subtilis and is characterized by the expressed bifido- and lactogenic properties. The obtained results show, that lactitol is a promising prebiotic for development of synbiotics on the basis of strains of the Bacillus genus bacteria.
