ABSTRACT
The objective of the present work was to study the distribution of tetramethylthiuram disulfide (TMTD) in the body of warm-blooded animals as exemplified by white rats. TMTD was administered intragastrically and detected in the unaltered form in other internal organs. It was found to accumulate in the largest amounts in the gastric contents, kidneys, and urine.
Subject(s)
Thiram/pharmacokinetics , Administration, Oral , Animals , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Gastrointestinal Contents/chemistry , Kidney/metabolism , Male , Rats , Spectrophotometry, Ultraviolet , Thiram/urine , Tissue DistributionABSTRACT
Optimal conditions for the identification and quantitative determination of tetraethyltiuram disulfide in "fresh" and putrefactive tissues of cadaverous liver are described for the purpose of TLC, HPLC, and IK-spectrophotometry following extraction of the compound of interest with ethyl acetate and its purification on a silicagel L column, 40/100 mcm. The persistence of tetraethyltiuram disulfide in the cadaverous tissues was evaluated. It was shown that the period during which tetraethyltiuram disulfide can be detected in the autopsied tissues decreases from 203 to 28 days with a rise in temperature from -15 degrees C to +36 degrees C.
