ABSTRACT
ADCC reaction (antibody dependent cellular cytotoxicity), ADCC capacity and ADCC steroid sensitivity examinations were performed in 20 children with tumorous or haematological diseases, 10 children with autoimmune diseases, and appropriate controls, in order to establish the killer function and steroid sensitivity. In the above diseases a study was also made of the correlation of the individual reactions with the duration of steroid therapy. The two patient groups did not exhibit a significant difference from the controls as concerns the ADCC reaction and ADCC capacity. In the group of tumorous or malignant haematological diseases the steroid sensitivity behaved in a different way, with sensitivity in 45%, moderate sensitivity in 20%, and steroid resistance in 35% of the patients. Steroid inhibition of the ADCC reaction was significantly decreased in the group of autoimmune patients. There was no correlation between ADCC reaction and ADCC steroid sensitivity or resistance in either group, and thus the ADCC steroid resistance or sensitivity and lymphocyte killer function proved to be independent. No correlation was found between the steroid sensitivity or resistance and the duration of steroid treatment.
Subject(s)
Antibody-Dependent Cell Cytotoxicity , Autoimmune Diseases/immunology , Hematologic Diseases/immunology , Killer Cells, Natural/immunology , Lymphocytes/immunology , Methylprednisolone/pharmacology , Adolescent , Antibody-Dependent Cell Cytotoxicity/drug effects , Child , Child, Preschool , Female , Humans , Infant , MaleABSTRACT
Beta-glucosidase was determined in leukocyte homogenates of a male child with Gaucher's disease and members of his family. An important difference in enzyme activity was found in the heterozygous gene carriers; in one obligate heterozygote, the mother, a high residual enzyme capacity was detected. Various methods of enzyme determination using various substrates are recommended for the detection of the gene carrier condition.