Tissue engineering construction from 3D porous ceramic carriers and multipotent stromal cells for the repair of bone tissue defects.

The tissue engineering construction was developed from human bone marrow multipotent stromal cells and 3D porous foamed-ceramic carriers of a zirconium oxide-aluminum oxide system. The carriers had no cytotoxic activity and were potent in maintaining the cell adhesion and proliferation. We developed the method for inoculation and cultivation of bone marrow multipotent stromal cells on these carriers. The optimal time of incubation to obtain a tissue engineering construction was estimated. Bone marrow multipotent stromal cells could be cultured at a depth of 9 mm from the edge of the matrix. The tissue engineering construction holds promise for the repair of extensive defects in bone tissue.