Phytochemical profiling of Symplocos tanakana Nakai and S. sawafutagi Nagam. leaf and identification of their antioxidant and anti-diabetic potential.

Symplocos sp. contains various phytochemicals and is used as a folk remedy for treatment of diseases such as enteritis, malaria, and leprosy. In this study, we discovered that 70% ethanol extracts of Symplocos sawafutagi Nagam. and S. tanakana Nakai leaves have antioxidant and anti-diabetic effects. The components in the extracts were profiled using high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry; quercetin-3-O-(6''-O-galloyl)-ß-d-galactopyranoside (6) and tellimagrandin II (7) were the main phenolic compounds. They acted as strong antioxidants with excellent radical scavenging activity and as inhibitors of non-enzymatic advanced glycation end-products (AGEs) formation. Mass fragmentation analysis demonstrated that compounds 6 and 7 could form mono- or di-methylglyoxal adducts via reaction with methylglyoxal, which is a reactive carbonyl intermediate and an important precursor of AGEs. In addition, compound 7 effectively inhibited the binding between AGE2 and receptor for AGEs as well as the activity of α-glucosidase. Enzyme kinetic study revealed that compound 7 acts as a competitive inhibitor of α-glucosidase, through interaction with the active site of the enzyme. Therefore, compounds 6 and 7, the major constituents of S. sawafutagi and S. tanakana leaves, are promising for developing drugs for preventing or treating diseases caused by aging and excessive sugar consumption.

Body positivity through creative immersion: A qualitative study of women's pole dancing experiences.

Perceptions of body image are shaped by sociocultural influences and have been extensively examined in relation to physical and psychological health outcomes. Our study explores concepts of body image manifested through experiences of "flow" among women in South Korea who participate in pole dancing as a recreational physical activity. A qualitative research design incorporating the hermeneutic phenomenological approach revealed that pole dancing serves as a creative medium which embodies a central role in the lives of women of diverse cultural and socioeconomic backgrounds by promoting body appreciation, self-acceptance, improving physical health, enhancing confidence, providing a sense of enjoyment, and establishing social support. Our findings affirm the significance of leisure based physical activities that emphasize functionality and promote positive body image such as pole dancing may be an effective self-care strategy which can be incorporated in health interventions for women within community programs on an international scale.

Phytoestrogen Coumestrol Selectively Inhibits Monoamine Oxidase-A and Amyloid ß Self-Aggregation.

Pueraria lobata leaves contain a variety of phytoestrogens, including flavonoids, isoflavonoids, and coumestan derivatives. In this study, we aimed to identify the active ingredients of P. lobata leaves and to elucidate their function in monoamine oxidase (MAO) activation and Aß self-aggregation using in vitro and in silico approaches. To the best of our knowledge, this is the first study to elucidate coumestrol as a selective and competitive MAO-A inhibitor. We identified that coumestrol, a coumestan-derivative, exhibited a selective inhibitory effect against MAO-A (IC50 = 1.99 ± 0.68 µM), a key target protein for depression. In a kinetics analysis with 0.5 µg MAO-A, 40-160 µM substrate, and 25 °C reaction conditions, coumestrol acts as a competitive MAO-A inhibitor with an inhibition constant of 1.32 µM. During an in silico molecular docking analysis, coumestrol formed hydrogen bonds with FAD and pi-pi bonds with hydrophobic residues at the active site of the enzyme. Moreover, based on thioflavin-T-based fluorometric assays, we elucidated that coumestrol effectively prevented self-aggregation of amyloid beta (Aß), which induces an inflammatory response in the central nervous system (CNS) and is a major cause of Alzheimer's disease (AD). Therefore, coumestrol could be used as a CNS drug to prevent diseases such as depression and AD by the inhibition of MAO-A and Aß self-aggregation.

"When I Sing, I Feel Peaceful": Experiences of Older Choral Singers From North Korea.

North Korean (NK) defectors fled their homeland and traveled thousands of miles in search of a better life. This qualitative study explored the experiences of older NK defectors who were part of a serious leisure choral group. In-depth interviews were conducted with eight NK defector members of a South Korean choir group, from which four central themes emerged: (a) conquering adversity and promoting health; (b) making good friends and having great adventures; (c) providing an arena to develop a new identity; and (d) developing proficiency through vigorous efforts. Involvement in choral singing as a serious leisure activity assisted the members in overcoming the various adversities they were facing, promoted their physical and mental health, and helped them develop comradery and new social identities.