ABSTRACT
BACKGROUND/AIM: This study investigated the utility of C-C motif chemokine ligand 20 (CCL20) expression in saliva as a biomarker for oral squamous cell carcinoma (OSCC) and also examined the associated microbiome. MATERIALS AND METHODS: The study group included patients with OSCC or oral potentially malignant disorder (OPMD), and healthy volunteers (HVs). microarray and qRT-PCR were used to compare salivary CCL20 expression levels among groups. Data on CCL20 levels in oral cancer tissues and normal tissues were retrieved from a public database and examined. Furthermore, next-generation sequencing was used to investigate the salivary microbiome. RESULTS: A significant increase in the expression level of CCL20 was observed in both OSCC tissues and saliva from patients with oral cancer. Fusobacterium was identified as the predominant bacteria in OSCC and correlated with CCL20 expression level. OSCC screening based on salivary CCL20 expression enabled successful differentiation between patients with OSCC and HVs. CONCLUSION: CCL20 expression may be a useful biomarker for OSCC.
Subject(s)
Biomarkers, Tumor/metabolism , Carcinoma, Squamous Cell/diagnosis , Chemokine CCL20/metabolism , Mouth Neoplasms/diagnosis , Case-Control Studies , Female , Humans , Male , Middle AgedABSTRACT
BACKGROUND/AIM: This study aimed to identify novel biomarkers for oral squamous cell carcinoma (OSCC) screening to improve the survival rate of patients with oral cancer. MATERIALS AND METHODS: We investigated differential salivary gene expression in patients with OSCC, those with oral potentially malignant disorders (OPMDs), and healthy volunteers (HVs). CPLANE1 was selected for further investigation by microarray analysis. We used quantitative reverse transcription PCR (qRT-PCR) to determine CPLANE1 expression levels in the saliva. The expression of CPLANE1 in normal and oral cancer tissues was analyzed using the Gene Expression database of Normal and Tumor tissues. RESULTS: qRT-PCR analysis of saliva samples showed that CPLANE1 expression levels were significantly higher in OSCC patients than in HVs and OPMDs patients. Furthermore, we developed a screening test for OSCC using CPLANE1 and showed that it had good accuracy. CONCLUSION: Salivary CPLANE1 could be a useful biomarker for OSCC screening and early detection.
Subject(s)
Biomarkers, Tumor/genetics , Carcinoma, Squamous Cell/diagnosis , Membrane Proteins/genetics , Mouth Neoplasms/diagnosis , Saliva/chemistry , Up-Regulation , Adult , Aged , Aged, 80 and over , Carcinoma, Squamous Cell/genetics , Carcinoma, Squamous Cell/pathology , Case-Control Studies , Early Detection of Cancer , Female , Gene Expression Regulation, Neoplastic , Humans , Leukoplakia, Oral/genetics , Lichen Planus, Oral/genetics , Male , Middle Aged , Mouth Neoplasms/genetics , Mouth Neoplasms/pathology , Neoplasm Staging , Sensitivity and SpecificityABSTRACT
Mucinous cystadenoma of the appendix is a type of appendiceal mucocele and a rather rare condition usually found incidentally in the course of other abdominal surgery. A previous evaluation of 73 appendiceal mucoceles showed that this disease was often associated with adenocarcinoma and other epithelial atypia. This observation suggested that patients with mucinous cystadenoma of the appendix also have some underlying disorders. However, non-epithelial changes associated with appendiceal mucocele have not been reported so far. In this study, we presented for the first time a mucinous cystadenoma of the appendix associated with muscular and neuromatous hyperplasia.
Subject(s)
Appendiceal Neoplasms/pathology , Cystadenoma, Mucinous/pathology , Muscle, Smooth/pathology , Adult , Appendiceal Neoplasms/complications , Appendiceal Neoplasms/surgery , Cystadenoma, Mucinous/complications , Cystadenoma, Mucinous/surgery , Female , Humans , Hyperplasia/complications , Treatment OutcomeABSTRACT
BACKGROUND: We previously detected that deltaNp63, a human p53 homologue, is an oncogene amplified in squamous cell carcinomas (SCC) including esophageal SCC. Subsequently, we examined global patterns of gene expression in cancer cells following deltaNp63 gene introduction using an oligonucleotide microarray approach. We identified S100A2, a Ca2+-binding protein, as a novel downstream mediator of deltaNp63. MATERIALS AND METHODS: In this study, we examined S100A2 expression in esophageal SCC cell lines and primary SCCs using Northern analysis. RESULTS: We found that 2 out of 8 (25%) cell lines and 14 out of 30 primary esophageal cancers (47%) showed S100A2 gene overexpression compared to paired normal tissues. To examine a possible relationship between S100A2 overexpression and clinicopathological features, we proceeded with statistical analysis. S100A2 overexpression was significantly-associated with higher age in esophageal SCC (p=0.0434). Interestingly, S100A2-overexpressing cancers showed a trend toward preferentially developing lymph node metastases and distant metastases (p=0.111 and 0.178, respectively). CONCLUSION: These results suggested that S100A2 might be related to the progression of esophageal SCC.
