Isotretinoin's Effect on Fasting Lipid Profile in Acne Patients.

OBJECTIVE: To determine the isotretinoin's effect on fasting lipid profile in patients with acne. STUDY DESIGN: Observational study. Place and Duration of the Study: Outpatient Department of Dermatology, Dow International Medical College, Dow University of Health Sciences, Karachi, Pakistan, from 22nd June to 21st December 2022. METHODOLOGY: Patients of clinically moderate and severe acne were selected and prescribed a dose of 0.5mg /kg cap isotretinoin for 6 months. They were advised to get a fasting lipid profile at the baseline and then after two months of isotretinoin therapy. National Cancer Institute Common Terminology Criteria for Adverse Events v5.0 grading system and Adult Treatment Panel III were used for the grading of abnormalities. McNemar Bowker test was used to assess the difference in variables [serum triglycerides (TGs), cholesterol, high-density lipoproteins (HDL), and low-density lipoproteins (LDL)] at the baseline and after 2 months follow-up. RESULTS: A total of 214 patients were evaluated. After 2 months of isotretinoin therapy, TGs and cholesterol levels were elevated to higher grade in 2% of the patients. Likewise in 1% of patients, LDL levels rised to higher grade. Moreover, HDL levels declined to lower grade in 2% of the patients taking isotretinoin. CONCLUSION: Insignificant alterations in the various serum lipid parameters were observed in acne patients during isotretinoin therapy. It is advisable to obtain a baseline fasting lipid profile in all acne patients on isotretinoin and repeated in those with baseline abnormal levels and in patients with a clinical sign of metabolic syndrome and a family history of dyslipidemias. KEY WORDS: Acne, Hyperlipidemias, Isotretinoin, Laboratory monitoring.

Biocompatibility performance evaluation of high flux hydrophilic CO3Ap/HAP/PSF composite membranes for hemodialysis application.

Carbonate apatite/hydroxyapatite (CO3Ap/HAP) additive was obtained by calcination of wasted chicken bones at 900°C. Intermolecular attraction exists between CO3Ap/HAP additive and blended polysulfone (PSF) polymer. Electron dispersive X-ray (EDX) and FTIR analysis were carried out to check the elemental composition and bonding chemistry of prepared additive. The instantaneous demixing process generated consistent finger-like networks in CO3Ap/HAP/PSF-based composite membranes while sponge-like structure was shown by PSF as revealed by SEM images. The increase in weight % of additive loading is also confirmed by EDX analysis. Furthermore, the interaction mechanism of CO3Ap/HAP additive with polysulfone medium was analyzed by FTIR exploration. The water absorption experiment defined a 93% expansion in hydrophilic performance. Change in porosity occurs with additive loading and pure water permeation flux improved up to 11 times. Approximately, antifouling results revealed that 87% of water flux was recovered after treating with a protein solution, whereas a 30% improvement in antifouling capability in case of bovine serum albumin solution occurred. In vitro cytotoxicity, and clotting times study was carried out to evaluate virulent behavior and anticoagulation activity of formulated membranes.