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Active/inactive dual-probe system for selective photoaffinity labeling of small molecule-binding proteins.
Sakurai, Kaori; Tawa, Masaki; Okada, Ayumi; Yamada, Rika; Sato, Noriyuki; Inahara, Masahiro; Inoue, Maia.
Chem Asian J ; 7(7): 1567-71, 2012 Jun.
Article in English | MEDLINE | ID: mdl-22514195

ABSTRACT

Two are better than one: A new approach to selective photoaffinity labeling is described in which a bioactive probe is used in combination with its inactive analog as a scavenger of nonspecific proteins.


Subject(s)
Carbonic Anhydrase II/metabolism , Photoaffinity Labels/analysis , Serum Albumin, Bovine/metabolism , Small Molecule Libraries/metabolism , Animals , Cattle , HeLa Cells , Humans , Ligands , Photoaffinity Labels/metabolism , Protein Binding , Small Molecule Libraries/chemistry
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