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J Clin Microbiol ; 43(6): 2940-3, 2005 Jun.
Article in English | MEDLINE | ID: mdl-15956426

ABSTRACT

We studied 13 extended-spectrum beta-lactamase (ESBL)-producing enteropathogenic Escherichia coli isolates from children suffering acute diarrhea in Uruguay. ESBL characterization in crude extracts showed a single band at pI 5.4. PCR amplification and sequencing data allowed identification of blaPER-2 and blaTEM-116. Retrospective analysis suggests that these strains were disseminated in the community, even if unnoticed, prior to their access to the hospital environment more than a decade ago.


Subject(s)
Diarrhea/epidemiology , Diarrhea/microbiology , Escherichia coli Proteins/genetics , Escherichia coli/drug effects , Escherichia coli/isolation & purification , beta-Lactamases/genetics , Anti-Bacterial Agents/pharmacology , Cephalosporin Resistance , Child, Preschool , Escherichia coli/enzymology , Escherichia coli/genetics , Escherichia coli Infections/epidemiology , Escherichia coli Infections/microbiology , Escherichia coli Proteins/metabolism , Humans , Infant , Microbial Sensitivity Tests , Molecular Sequence Data , Polymerase Chain Reaction/methods , Sequence Analysis, DNA , Uruguay/epidemiology , beta-Lactamases/metabolism
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