The experience of using prompting technology from the perspective of people with Dementia and their primary carers.

OBJECTIVES: People who are living with dementia typically experience difficulties in completing multi-step, everyday tasks. However, digital technology such as touchscreen tablets provide a means of delivering concise personalised prompts that combine audio, text and pictures. This study was one component of a broader, mixed methods study that tested how an application (app) -based prompter running on a touchscreen tablet computer could support everyday activities in individuals with mild to moderate dementia. In this study we set out to understand the experiences of people living with dementia and their primary carer in using the prompter over a four-week period. METHOD: We collected qualitative data using semi-structured interviews from 26 dyads, composed of a person living with dementia and their carer. Dyads were interviewed at the start and end of this period. Transcripts were then analysed using thematic analysis. RESULTS: The study identified three overarching themes related to: participants' attitudes towards the technology; their judgements about how useful the prompter would be; and the emotional impact of using it. CONCLUSION: Consistent with the Technology Acceptance Model, carers and participants were influenced by their approaches to technology and determined the usefulness of the prompter according to whether it worked for them and fitted into their routines. In addition, participants' decisions about using the prompter were also determined by the extent to which doing so would impact on their self-identity.

Effects of fixatives on stable isotopes of fish muscle tissue: implications for trophic studies on preserved specimens.

Isotopic ecology has been widely used to understand spatial connectivity and trophic interactions in marine systems. However, its potential for monitoring an ecosystem's health and function has been hampered by the lack of consistent sample storage and long-term studies. Preserved specimens from museum collections are a valuable source of tissue for analyses from ancient and pre-modern times, but isotopic signatures are known to be affected by commonly used fixatives. The aim of the present study was to understand the effects of fixatives on isotopic signatures of bulk tissue (δ13 Cm and δ15 Nm ) and amino acids (δ13 CAA and δ15 NAA ) of fish muscle and to provide correction equations for the isotopic shifts. Two specimens of each: blue cod (Parapercis colias), blue warehou (Seriolella brama), and king salmon (Oncorhynchus tschawytscha) were sampled at five locations along their dorsal musculature, at four time periods: (1) fresh, (2) after 1 month preserved in formalin, and after (3) 3 and (4) 12 months fixed in either ethanol or isopropanol. Lipid content was positively correlated with C:N ratio (r² = 0.83) and had a significant effect on δ13 C after treatments, but not on δ15 N. C:N ratio (for δ13 Cm ) and percent N (for δ15 Nm ) from preserved specimens contributed to the most parsimonious mixed models, which explained 79% of the variation due to fixation and preservation for δ13 C and 81% for δ15 N. δ13 CAA were generally not affected by fixatives and preservatives, while most δ15 NAA showed different signatures between treatments. δ15 NAA variations did not affect the magnitude of differences between amino acids, allowing scientists to retrieve ecological information (e.g., trophic level) independently of time under preservation. Corrections were applied to the raw data of the experiment, highlighting the importance of δ13 Cm and δ15 Nm correction when fish muscle tissues from wet collections are compared to fresh samples. Our results make it possible to retrieve δ13 Cm , δ15 Nm , δ13 CAA , and δ15 NAA from museum specimens and can be applied to some of the fundamental questions in ecology, such as trophic baseline shifts and changes in community's food web structure through time.

Role of ecology and phylogeny in determining tapeworm assemblages in skates (Rajiformes).

An understanding of the mechanisms that determine host and parasite relationships is a central aim in parasitology. Association of a parasite species with a host species may be influenced primarily by phylogenetic constraints that cause parasite species to co-speciate with their host species, or predominantly by ecological parameters that influence all other co-evolutionary scenarios. This study aimed to investigate the role of co-speciation as well as other co-evolutionary scenarios in influencing the assemblages of tapeworm parasites (marine cestodes) in skate hosts (Rajiformes) using a modification of the PACo (Procrustean Approach to Cophylogeny) method. The study found that phylogeny and host ecology are both significant predictors of skate-tapeworm relationships, implying that co-speciation as well as other co-evolutionary scenarios are shaping these associations. The study also investigated the key ecological parameters influencing host-switching and found that host diet, distribution depth, average body size and geographical location have a combined effect. Given the importance of parasites in ensuring healthy and stable marine ecosystems, the findings of this study have implications for conservation management worldwide.

Predicting solute partitioning in lipid bilayers: Free energies and partition coefficients from molecular dynamics simulations and COSMOmic.

Quantitative predictions of biomembrane/water partition coefficients are important, as they are a key property in pharmaceutical applications and toxicological studies. Molecular dynamics (MD) simulations are used to calculate free energy profiles for different solutes in lipid bilayers. How to calculate partition coefficients from these profiles is discussed in detail and different definitions of partition coefficients are compared. Importantly, it is shown that the calculated coefficients are in quantitative agreement with experimental results. Furthermore, we compare free energy profiles from MD simulations to profiles obtained by the recent method COSMOmic, which is an extension of the conductor-like screening model for realistic solvation to micelles and biomembranes. The free energy profiles from these molecular methods are in good agreement. Additionally, solute orientations calculated with MD and COSMOmic are compared and again a good agreement is found. Four different solutes are investigated in detail: 4-ethylphenol, propanol, 5-phenylvaleric acid, and dibenz[a,h]anthracene, whereby the latter belongs to the class of polycyclic aromatic hydrocarbons. The convergence of the free energy profiles from biased MD simulations is discussed and the results are shown to be comparable to equilibrium MD simulations. For 5-phenylvaleric acid the influence of the carboxyl group dihedral angle on free energy profiles is analyzed with MD simulations.

First Report of Bacterial Blight of Sugar Beet Caused by Pseudomonas syringae pv. aptata in Georgia, USA.

Sugar beet (Beta vulgaris L.) is not currently a commercial crop in Georgia, but experimental plantings as a winter rotational crop are promising in terms of yield and industrial sugar production (T. Brenneman, personal communication). A disease outbreak of suspected bacterial origin occurred in sugar beet plots (experimental lines Beta Seed energy beet 'BTS ENC115,' 'BTS EGC184,' 'BTS EGC195,' and 'BTS 1EN6702') in Tift Co., GA, in December 2012, at ~35% incidence. Foliar symptoms included circular to irregular spots, each with a tan center and dark margin. Ten leaves/experimental line with leaf spot symptoms were collected, and bacterial isolations made on King's B agar medium. After 48 h of incubation, cream-colored, fluorescent yellow, round colonies with smooth margins were isolated. The isolates were each gram negative, oxidase negative, non-pectolytic on potato, arginine dihydrolase negative, produced levan, and gave a hypersensitivity response (HR) on tobacco. These characteristics indicated that the isolates belonged to Pseudomonas syringae van Hall LOPAT group Ia (3). The 16S-23S rRNA (internal transcribed regions) (1) from four foliar isolates (SB-1, SB-2, SB-3, and SB-4), one/experimental line, was amplified, and the resultant PCR products were sequenced and BLAST searched in GenBank. The 16S-23S rRNA sequences matched those of P. syringae pv. syingae (Pss) (KF023189) and P. syringae pv. aptata (Psa) (AY342167.1) with 96 to 98% and 97 to 99% sequence identity, respectively. Also, the percent similarity of the 16S-23S rRNA sequences among the four isolates was >99% (KJ922021 to 24 for SB-1 to SB-4, respectively). The four test isolates also had ≤89 and ≤99% similarity with Pss and Psa, respectively, when tested with BIOLOG (Hayward, CA). In addition, four sugarbeet isolates along with a type strain of Psa (NCPPB 3539) were amplified using a PCR primer pair that detected the presence of the avrPphE gene, an avirulence gene present in Psa but absent in Pss (2). The type strain of Pss (NCPPB 1770) was not amplified using this primer pair. BOX-PCR analysis gave identical banding patterns for the four isolates as that of a type strain of Psa. In two independent experiments, 3-week-old seedlings of the sugar beet cv. Beta EGR099 (n = 10 seedlings/isolate/experiment) were spray-inoculated with a sterilized water suspension of 1 × 108 CFU/ml of each of the isolates. All of the inoculated seedlings developed symptoms (water-soaked lesions that developed into necrotic spots) 10 days after inoculation (DAI) in greenhouse conditions (~30°C and ~80% RH). All of the seedlings inoculated with the type strain of Psa also produced typical bacterial blight symptoms at 10 DAI. In contrast, five control seedlings inoculated with sterilized water remained asymptomatic, and target bacterial colonies were not re-isolated from the leaves of these plants. Bacterial colonies were re-isolated from symptomatic seedlings, and showed similar characteristics based on physiological tests, BIOLOG profile, BOX-PCR analysis, and positive amplification with the avrPphE PCR assay, which indicated that these strains were Psa. To our knowledge, this is the first report of Psa in sugarbeet in Georgia. The fact that a Psa strain was also isolated from a sugar beet seed lot (data not shown) suggested that the pathogen may have been introduced on contaminated seeds. Knowledge of the presence of Psa in the agro-ecosystem of Georgia may encourage scientists to implement integrated management practices for this pathogen. References: (1) C. Guasp et al. Int. J. Syst. Evol. Microbiol. 50:1629, 2000. (2) Y. Inoue and Y. Takikawa. Page 687 in: Presentations 6th Int. Conf. Pseudomonas syringae Pathovars and Related Pathogens, 2003. (3) R. A. Lelliot et al. J. Appl. Bacteriol. 29:470, 1966.

When should we expect early bursts of trait evolution in comparative data? Predictions from an evolutionary food web model.

Conceptual models of adaptive radiation predict that competitive interactions among species will result in an early burst of speciation and trait evolution followed by a slowdown in diversification rates. Empirical studies often show early accumulation of lineages in phylogenetic trees, but usually fail to detect early bursts of phenotypic evolution. We use an evolutionary simulation model to assemble food webs through adaptive radiation, and examine patterns in the resulting phylogenetic trees and species' traits (body size and trophic position). We find that when foraging trade-offs result in food webs where all species occupy integer trophic levels, lineage diversity and trait disparity are concentrated early in the tree, consistent with the early burst model. In contrast, in food webs in which many omnivorous species feed at multiple trophic levels, high levels of turnover of species' identities and traits tend to eliminate the early burst signal. These results suggest testable predictions about how the niche structure of ecological communities may be reflected by macroevolutionary patterns.

Niche diversification follows key innovation in Antarctic fish radiation.

Antarctic notothenioid fishes provide a fascinating evolutionary laboratory for the study of adaptive radiation, as their diversification is linked to both isolation in an extreme environment and a key innovation that allows them to exploit it. In this issue of Molecular Ecology, Rutschmann et al. (2011) evaluate how dietary niche differences have evolved in notothenioids: rarely, or repeatedly in multiple lineages. The authors use stable isotopes to measure species' use of benthic vs. pelagic resources and map resource use onto a molecular phylogeny. Their findings indicate that pelagic diets have evolved in multiple lineages in at least two families, indicating that dietary niche diversification has occurred repeatedly and in parallel.

Comparison of novel cannabinoid partial agonists and SR141716A in the guinea-pig small intestine.

The controversial nature of the CB(1) receptor antagonist, SR141716A, in the guinea-pig small intestine was investigated by comparing it with four analogues of Delta(8)-tetrahydrocannabinol (Delta(8)-THC): O-1184, O-1238, O-584 and O-1315. These compounds (10 - 1000 nM) inhibited the electrically-evoked contractions with a rank order of potency of O-1238>O-1184>O-584>O-1315. Log concentration-response curves for O-1238, O-1184 and O-1315 were significantly shifted to the right by SR141716A and the maxima were significantly less than that of the CB(1) agonist, WIN55212-2, an indication of partial agonism. Partial saturation of the triple bond in O-1184 to a cis double bond (O-1238) increased its potency as an agonist (pEC(50) from 6.42 to 7.63) and as an antagonist of WIN55212-2, (pK(B), from 8.36 to 9.49). Substitution of the terminal azide group by an ethyl group (O-584) or removal of the phenolic hydroxyl group (O-1315) had no significant effect on the agonist or antagonist potency. None of these analogues increased the twitch response in a manner resembling that of SR141716A. O-1184 (10 and 100 nM) shifted the log concentration-response curve of WIN55212-2 for inhibition of the twitch responses to the right with pK(B) values of 8.29 and 8.38, respectively. We conclude that these Delta(8)-THC analogues behave as partial agonists rather than silent antagonists at CB(1) binding sites in this tissue. There was no evidence of antagonism of endocannabinoids thus supporting the hypothesis that, in this tissue, SR141716A is an inverse agonist of constitutively active CB(1) receptors.

An outbreak of viral gastroenteritis associated with consumption of sandwiches: implications for the control of transmission by food handlers.

Although food handlers are often implicated as the source of infection in outbreaks of food-borne viral gastroenteritis, little is known about the timing of infectivity in relation to illness. We investigated a gastroenteritis outbreak among employees of a manufacturing company and found an association (RR = 14.1, 95% CI = 2.0-97.3) between disease and eating sandwiches prepared by 6 food handlers, 1 of whom reported gastroenteritis which had subsided 4 days earlier. Norwalk-like viruses were detected by electron microscopy or reverse transcriptase-polymerase chain reaction (RT-PCR) in stool specimens from several company employees, the sick food handler whose specimen was obtained 10 days after resolution of illness, and an asymptomatic food handler. All RT-PCR product sequences were identical, suggesting a common source of infection. These data support observations from recent volunteer studies that current recommendations to exclude food handlers from work for 48-72 h after recovery from illness may not always prevent transmission of Norwalk-like viruses because virus can be shed up to 10 days after illness or while exhibiting no symptoms.

Comparison of the effects of selected dressings on the healing of standardized abrasions.

OBJECTIVE: To find out which type of dressing (semipermeable film, hydrocolloid, conventional method, or no dressing) allowed abrasions to heal in the least amount of time and had the greatest decrease in wound area. DESIGN AND SETTING: A 4 x 9 factorial was used for this study. There were two independent variables with four levels and two dependent variables. Research was performed at the Athletic Training Research Laboratory at Indiana State University. SUBJECTS: Fourteen subjects (eight males, six females), ages 23 to 34 years, participated in this study. MEASUREMENTS: From daily photographs, the day the wounds were healed was determined. The photographs were also used to measure wound area on the first and last days of the study. Subjects received four treatments (dressings and control), and placement of the dressings was determined by random assignment. RESULTS: Data were analyzed using a repeated-measures multivariate analysis of variance to determine if differences existed among treatment groups for healing time and change in area. Student-Newman-Keuls post hoc testing was performed to determine specifically where the differences occurred. Our results indicate that healing time is affected by covering the wound, and area is decreased by using DuoDerm or Bioclusive. CONCLUSIONS: Bioclusive should be used in the athletic training setting. Bioclusive and DuoDerm are equally effective, but Bioclusive is less expensive. Bioclusive is more expensive than Coverlet, but it is also more effective in reducing the area of the wound.

Group psychotherapy for patients with dementia.

Some of the goals of our group psychotherapy sessions on the inpatient unit include (1) creation of an emotional climate of acceptance and warmth that helps patients learn to accept themselves and their feelings, (2) frequent intervention by the group facilitator/therapist to help facilitate social interaction for patients whose communication ability is impaired, (3) opportunity for patients to experience the feeling of belonging, of being part of a group, (4) opportunity for patients to ventilate feelings and rediscover mutual kinds of experience, (5) opportunity for patients to reminisce about past accomplishments and give new meaning to their current lives, and (6) creation of a platform for patients to achieve a sense of self by expressing personal opinions in an environment of respect and acceptance. The outcome of group therapy for demented as well as nondemented patients should be increased ability to cope with losses at several levels, promotion of new skills, increased adaptation skills, and increased ability to accept change. We also want patients to learn to express feelings and to realize that the expression of feelings can have a positive outcome (relief from repression, clarification of ambivalence, solutions, etc).

A cross-sectional analysis of family medicine publications in the indexed medical literature.

BACKGROUND: Publications are important to the academic development of the field of family medicine. Not all manuscripts written by family physicians or family medicine faculty are published in family medicine journals. The purpose of this study was to determine where manuscripts generated by US family medicine department faculty are being published. METHODS: A MEDLINE search was performed for all English language articles published in 1979 and 1989. The search technique involved identifying articles that included the words "family" and "medicine" or "family" and "practice" in the institution field of the MEDLINE index. All identified articles were then manually reviewed to confirm authorship by family medicine department faculty. Article types were classified according to previously published schemata. RESULTS: The research identified 178 articles from 1979 and 328 articles form 1989. In 1979, 52% of the articles had been published in family medicine journals. In 1989, the percentage was exactly 50%. Biomedical articles comprised the largest category (approximately half) of articles. Educational articles were the second most common type. CONCLUSIONS: The number of publications generated by family medicine faculty has grown, as has the number (but not the percentage) of articles published in non-family medicine journals.

Creating customer-oriented employees: the case in home health care.

Little empirical research has examined the organizational factors that influence the extent to which health care providers engage in customer-oriented behaviors. The authors examine the influence of role ambiguity, role conflict, and job satisfaction on the customer-oriented behaviors of home health care representatives. Managerial implications based on the study findings are discussed.

In vitro comparison of bleaching agents on the crowns and roots of discolored teeth.

Sixty teeth were stained in vitro to compare the decolorizing effect of three bleaching agents on the crowns and on the roots of teeth containing an intracoronal isolating base. With an intracoronal isolating base placed either at the cementoenamel junction (CEJ) or 2-mm below the CEJ, bleaching agents of Superoxol, sodium perborate, and a combination of the two were placed in the pulp chamber of teeth and the accesses sealed with IRM. Customized shade guides were used to evaluate the color changes of the crowns and roots. After three treatments, the combination technique was found to be more effective in decolorizing the crowns and roots of teeth than Superoxol or sodium perborate alone (p less than 0.05). No difference was found between the Superoxol group and the sodium perborate group. There was no difference in color shades of crowns in which the IRM base was placed either at the CEJ or 2-mm below the CEJ. The roots of all groups showed some degree of decoloration. Based on the bleaching effects on discolored roots, the effectiveness of this seal is questionable.

Comparison of the sealing ability of four obturation techniques.

This study compared the apical seal produced by four obturation techniques. The Canal Finder system was compared with lateral condensation, the Ultrafil system, and the sectional warm gutta-percha techniques. Forty-two anterior single-rooted teeth were instrumented. Ten teeth were not obturated and served as positive and negative controls. The apical seal was tested for leakage with 0.25% methylene blue dye. The teeth were shaved horizontally and dye penetration was measured with a Boley gauge. Results revealed there was no statistically significant difference among the four obturation techniques.

Response of the human eye to accidental exposure to sodium hypochlorite.

Sodium hypochlorite is used in endodontic therapy as an intracanal irrigant during hand, mechanical, and ultrasonic instrumentation of the root canal space. Regardless of the technique utilized, particular attention must be given to the appropriate and safe use of this chemical solution. A case is presented in which a 5.25% sodium hypochlorite solution was accidentally splashed in a patient's eyes during endodontic therapy. Ocular irrigation was performed immediately and continued for 10 min. The patient was promptly taken to an ophthalmologist for further treatment and follow-up. When chemical trauma of the eye by sodium hypochlorite solution is sustained, the entire surface of the affected eye(s) and the fornices must be thoroughly irrigated. This is best accomplished with a continuous stream of normal saline from a 1-I bag which is attached to an intravenous line with a 16-gauge Teflon catheter placement sleeve affixed to the distal end of the line. Arrangements must then be made for immediate evaluation, treatment, and consultation by an ophthalmologist.

An evaluation of the performance of ten commercial luminometers.

An assessment has been carried out of the relative performance of ten instruments for quantification of adenosine triphosphate (ATP) by the firefly luciferase assay. The instruments evaluated were Amersham Amerlite Analyser, Dynatech Tube Luminometer, Dynatech Multiplate Luminometer, Dynatech Camera Luminometer, Hamilton Lumicon, LKB 1250 Luminometer, LKB 1251 Luminometer, Lumac Biocounter M2010A, Turner 20 TD Luminometer and a prototype version of the CLEAR SpeedTech 2000. An 800-fold difference in sensitivity was found between the most sensitive (Lumac, Turner) and the least sensitive (Dynatech Tube) of the conventional instruments. The Dynatech Camera Luminometer which worked on a completely different principle to the other instruments was about 5000 times less sensitive than the best of the photomultiplier tube instruments. The relative sensitivity of the instruments was maintained regardless of whether solutions of ATP in water or trichloroacetic acid extracts of bacteria were analysed. An analysis of 960 ATP bioluminescence assays showed that data obtained from such measurements are normally distributed.