An optofluidic antenna for enhancing the sensitivity of single-emitter measurements.

Many single-molecule investigations are performed in fluidic environments, for example, to avoid unwanted consequences of contact with surfaces. Diffusion of molecules in this arrangement limits the observation time and the number of collected photons, thus, compromising studies of processes with fast or slow dynamics. Here, we introduce a planar optofluidic antenna (OFA), which enhances the fluorescence signal from molecules by about 5 times per passage, leads to about 7-fold more frequent returns to the observation volume, and significantly lengthens the diffusion time within one passage. We use single-molecule multi-parameter fluorescence detection (sm-MFD), fluorescence correlation spectroscopy (FCS) and Förster resonance energy transfer (FRET) measurements to characterize our OFAs. The antenna advantages are showcased by examining both the slow (ms) and fast (50 µs) dynamics of DNA four-way (Holliday) junctions with real-time resolution. The FRET trajectories provide evidence for the absence of an intermediate conformational state and introduce an upper bound for its lifetime. The ease of implementation and compatibility with various microscopy modalities make OFAs broadly applicable to a diverse range of studies.

Organic Molecules as Origin of Visible-Range Single Photon Emission from Hexagonal Boron Nitride and Mica.

The discovery of room-temperature single-photon emitters (SPEs) hosted by two-dimensional hexagonal boron nitride (2D hBN) has sparked intense research interest. Although emitters in the vicinity of 2 eV have been studied extensively, their microscopic identity has remained elusive. The discussion of this class of SPEs has centered on point defects in the hBN crystal lattice, but none of the candidate defect structures have been able to capture the great heterogeneity in emitter properties that is observed experimentally. Employing a widely used sample preparation protocol but disentangling several confounding factors, we demonstrate conclusively that heterogeneous single-photon emission at ∼2 eV associated with hBN originates from organic molecules, presumably aromatic fluorophores. The appearance of those SPEs depends critically on the presence of organic processing residues during sample preparation, and emitters formed during heat treatment are not located within the hBN crystal as previously thought, but at the hBN/substrate interface. We further demonstrate that the same class of SPEs can be observed in a different 2D insulator, fluorophlogopite mica.

Water Shortage Affects Vegetative and Reproductive Stages of Common Bean (Phaseolus vulgaris) Chilean Landraces, Differentially Impacting Grain Yield Components.

Water availability for agricultural use is currently a global problem that worsens with climate change in several regions of the world. Among grain legumes, common bean (Phaseolus vulgaris) is the most cultivated in the worldwide. The Chilean germplasm of common bean is characterized by tolerance to water stress. Here, we analyzed a selection of nine ancient Chilean landraces in regard to their drought tolerance, simulating optimal (OW) and restricted watering (RW) in a Mediterranean environment. Phenological, growth, and yield traits were recorded, and correlation analysis was performed. Accordingly, leaf temperature and osmotic potential were higher under RW, while the leaf chlorophyll content decreased in all landraces. Physiological maturity days and seed-filling days were lower in RW than in OW. This similarly occurred with the grain yield. The % yield reduction was negatively correlated with the % pod reduction and the relative rate of leaf expansion (RLAE) reduction. However, the 100-seed weight value was not significantly modified by water treatment (p > 0.05). For instance, landraces that preferred to fill the grain with a lower rate of leaf expansion showed a lower loss in grain yield under drought conditions. These results suggest that the resource partitioning between growing leaves, flowers, and developing pods in Chilean landraces is variable, affecting the common bean drought tolerance.

Does urban climate follow urban form? Analysing intraurban LST trajectories versus urban form trends in 3 cities with different background climates.

The current climate change trend urges the application of efficient spatial planning to mitigate the effects of urbanization on local urban warming. Nevertheless, how urban temperatures respond to urban form changes inside cities is still insufficiently understood. In this paper, we explored the relationship between urban form and diurnal space-time land surface temperature (LST) trends (2003-2019) in Beijing (continental climate), Cairo (arid) and Santiago (temperate). We analysed changes in land cover, white sky albedo (WSA), night-time lights (NL) and the enhanced vegetation index (EVI) inside areas representing clustered thermal performance (steady cold and hot spots and warming cold and hot spots). The structure of local climate zones (LCZs) was assessed for each LST trend. To test the relevance of other urban form dimensions, we analysed the hierarchical influence of the employed 2D metrics (i.e., built-up cover, WSA, NL and EVI) and additional 3D indicators (i.e., height and volume) on LST, applying machine learning classification and regression trees (CARTs) to Beijing's data. Despite diverse patterns of urban form change, cities in our sample present common LST trends, with thermal differences as a consequence of local climate. LCZs are composed of highly heterogeneous built-up areas inside LST trend categories. In the case of Beijing, LST is hierarchically driven by footprint, WSA and EVI. Moreover, by adding height and volume, urban form differences between LST trend classes that are not evident with 2D data were found. Our findings suggest that a compact green urban tissue is necessary to cope with the current trends of urban warming, taking into account city-specific measures based on the local background climate.

Intraurban heterogeneity of space-time land surface temperature trends in six climate-diverse cities.

Surface urban heat islands (SUHIs) are present in all cities, derived from their thermal properties. While looking at the spatiotemporal variability of land surface temperature (LST), there is still a gap in understanding patterns of change. In this paper, we analysed diurnal and nocturnal annual mean LST trends in continental (Beijing), temperate (Mexico City and Santiago), and arid (Cairo, Hyderabad, and Riyadh) cities employing 1 km MODIS data (2003-2019). Each time-series was assessed with the structure of a space-time cube. Hot and cold spots were detected for each year and the LST trends were analysed. Each pixel was classified into different space-time LST trends and their SUHIs were estimated. Cities exhibit trends of increasing temperatures in cold and hot spots for diurnal and nocturnal data. Temperatures are increasing faster in hot spots for diurnal and in cold spots for nocturnal scenes. Steady hot spots and warming hot spots exhibit the highest SUHIs for day and night. Our approach provides a framework to empirically delineate the spatial intraurban heterogeneity of LST patterns over time. This spatially explicit information provides insights into urban areas requiring heat mitigation strategies and can be used to monitor the performance of measures already implemented for climate adaptation.

Phenotypic Diversity and Productivity of Medicago sativa Subspecies from Drought-Prone Environments in Mediterranean Type Climates.

The phenotypic diversity and productivity of a diverse alfalfa (M. sativa subspp.) panel of cultivars, landraces and wild relatives with putative drought tolerance were evaluated in two Mediterranean environments (central Chile and Southern Australia). In Chile, 70 accessions were evaluated in rainfed conditions and in Australia 30 accessions under rainfed and irrigated conditions, during three growing seasons. Large phenotypic variation was observed among and within subspecies for NDVI, stem length, intercepted PAR and forage yield. Principal component analysis indicated that the first two principal components (PC) accounted for 84.2% of total variance; fall dormancy, taxa, and breeding status were closely related to the agronomical performance of alfalfa accessions. Forage yield varied largely among accessions across years and locations. A linear relationship was found between annual forage yield and annual water added to the experiments (R2 = 0.60, p < 0.001). The GxE analysis for forage yield allowed the detection of the highest yielding accessions for each of the two mega-environments identified. The accessions CTA002 and CTA003 showed greater forage yield in both Chile and Australia environments. It is concluded that new breeding lines derived from crosses between cultivated alfalfa (M. sativa subsp. sativa) and wild relatives belonging to the primary (M. sativa subsp. falcata) and tertiary (M. arborea) gene pool, achieve outstanding agronomical performance in drought-prone environments.

Urban form datasets of 194 cities delineated based on the contiguous urban fabric for 1990 and 2015.

The present research datasets were processed for the article "The global homogenization of urban form. An assessment of 194 cities across time" [1]. They consist of land cover spatial layers, longitude and latitude point data and tabulated data with computed landscape metrics and the characterization of urban form of 194 cities for 1990 and 2015. Contiguous urban fabric at 30 m spatial resolution was derived from the Atlas of Urban Expansion database for 1990 and 2015 [2]. Landscape metrics were computed as quantitative measures of composition and spatial arrangement of each city and dimensions of the database were reduced employing correlation and principal components analysis. Hierarchical clustering was employed to group cities according to the similarity of their urban form and analysis of variance was applied to test for significant differences between them. The spatial layers contained in this article can be complemented with past and future land cover data to model urban form change at broader temporal scales. The landscape metrics are useful for cross-city comparisons at regional, national and global levels in combination with other complementary indicators. The datasets are valuable for urban planners, urban ecologists, NGO's, decision makers and other with interest on local and global landscape change in urban areas, particularly urban expansion and its impacts.

Understanding the Complexity of Cold Tolerance in White Clover using Temperature Gradient Locations and a GWAS Approach.

White clover ( L.) is the most important grazing perennial forage legume in temperate climates. However, its limited capacity to survive and restore growth after low temperatures during winter constrains the productivity and wide adoption of the crop. Despite the importance of cold tolerance for white clover cultivar development, the genetic basis of this trait remains largely unknown. Hence, in this study, we performed the first genome-wide association study (GWAS) analyses in white clover to identify quantitative trait loci (QTL) for cold-tolerance-related traits. Seeds from 192 divergent genotypes from six populations in the Patagonia region of South America were collected and seed-derived plants were further clonally propagated. Clonal trials were established in three locations representing temperature gradient associated with elevation. Given the allotetraploid nature of the white clover genome, distinct genetic models (diploid and tetraploid) were tested. Only the tetraploid parameterization was able to detect the 53 loci associated with cold-tolerance traits. Out of the 53 single nucleotide polymorphism (SNP) trait associations, 17 controlled more than one trait or were stable across multiple sites. This work represents the first report of QTL for cold-tolerance-related traits, providing insights into its genetic basis and candidate genomic regions for further functional validation studies.

Monitoring the effects of land cover change on the supply of ecosystem services in an urban region: A study of Santiago-Valparaíso, Chile.

Mankind's quest for well-being results in continuous pressure to transform landscapes, with said transformation driven by land use changes, urbanization, production activity, and protective measures in addition to climate variability and other environmental drivers. The relationship between anthropogenic landscape changes and the provision of ecosystem services (ES) is a topic of increasing interest in Latin America. In Chile, land cover changes due to increased urbanization and forestry, and expansion of agricultural land, in addition to conservation initiatives as a part of land planning, have been intensive in the last few decades. In this study, the effects of anthropogenic landscape changes on the supply of ES were analyzed for the urban region of Santiago-Valparaiso (Chile) using a method based on expert consultation and land cover change assessment. A pool of experts scored the potential of specific land covers to provide certain ES. The results enabled calculation and mapping of changes in the potential of the landscape to supply ES. The aforementioned changes over a period of 15 years were evaluated. The results indicate a tenuous balance between positive and negative changes to the supply of ES derived from land cover changes. Understanding and reporting how these processes occur in urban regions contributes to the conservation of valuable landscapes through spatial planning tools, especially in areas close to housing developments and sensitive ecosystems.

Observation of dipolar transport in one-dimensional photonic lattices.

We experimentally study the transport properties of dipolar and fundamental modes on one dimensional (1D) coupled waveguide arrays. By carefully modulating a wide optical beam, we are able to effectively excite dipolar or fundamental modes to study discrete diffraction (single-site excitation) and gaussian beam propagation (multi-site excitation plus a phase gradient). We observe that dipolar modes experience a larger spreading area due to an effective larger coupling constant, which is found to be more than two times larger than the one for fundamental modes. Additionally, we study the effect of non-diagonal disorder and find that while fundamental modes are already trapped on a weakly disorder array, dipoles are still able to propagate across the system.

A Heat Vulnerability Index: Spatial Patterns of Exposure, Sensitivity and Adaptive Capacity for Santiago de Chile.

Climate change will worsen the high levels of urban vulnerability in Latin American cities due to specific environmental stressors. Some impacts of climate change, such as high temperatures in urban environments, have not yet been addressed through adaptation strategies, which are based on poorly supported data. These impacts remain outside the scope of urban planning. New spatially explicit approaches that identify highly vulnerable urban areas and include specific adaptation requirements are needed in current urban planning practices to cope with heat hazards. In this paper, a heat vulnerability index is proposed for Santiago, Chile. The index was created using a GIS-based spatial information system and was constructed from spatially explicit indexes for exposure, sensitivity and adaptive capacity levels derived from remote sensing data and socio-economic information assessed via principal component analysis (PCA). The objective of this study is to determine the levels of heat vulnerability at local scales by providing insights into these indexes at the intra city scale. The results reveal a spatial pattern of heat vulnerability with strong variations among individual spatial indexes. While exposure and adaptive capacities depict a clear spatial pattern, sensitivity follows a complex spatial distribution. These conditions change when examining PCA results, showing that sensitivity is more robust than exposure and adaptive capacity. These indexes can be used both for urban planning purposes and for proposing specific policies and measures that can help minimize heat hazards in highly dynamic urban areas. The proposed methodology can be applied to other Latin American cities to support policy making.

Transport in Sawtooth photonic lattices.

We investigate, theoretically and experimentally, a photonic realization of a Sawtooth lattice. This special lattice exhibits two spectral bands, with one of them experiencing a complete collapse to a highly degenerate flat band for a special set of inter-site coupling constants. We report the observation of different transport regimes, including strong transport inhibition due to the appearance of the non-diffractive flat band. Moreover, we excite localized Shockley surface states residing in the gap between the two linear bands.

Efecto de la ingestión de Banisteriopsis caapi y Psychotria viridis 'Binomio ayahuasca' en el hipocampo del cerebro de ratas / Effect of Banisteriopsis caapi and Psychotria viridis "Binomio ayahuasca" ingestion in the rat hippocampus

Objetivo: Evaluar el efecto de la ingestión de Banisteriopsis caapi y Psychotria viridis 'Binomio ayahuasca' en el hipocampo de cerebro de ratas. Diseño: Estudio experimental, descriptivo, analítico, transversal. Institución: Instituto de Investigación en Ciencias Farmacéuticas y Recursos Naturales, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú. Material biológico: Ratas. Intervenciones: Administración del binomio ayahuasca por vía orogástrica a ratas albinas adultas de la especie Rattus novergicus y de cepa Holtzman. A siete grupos de cinco con un peso promedio de 240 ± 30 g se les administró (GI) blanco, (GII) 2,5 mL diazepam, (GIII) 0,7 mL solución de Banisteriopsis caapi, (GIV) 0,7 mL Psychotria viridis, y a los grupos (GV), (GVI) y (GVII) se administró 0,7 mL, 3,5 mL y 7,0 mL de solución del binomio ayahuasca, respectivamente. Principales medidas de resultados: Macroscópicos: comportamiento de reflejos y actividad motora. Microscópicos: número de células piramidales y granulosas, y desorganización celular. Resultados: En el tamizaje fitoquímico del extracto se caracterizó presencia de alcaloides, antraquinonas, triterpenoides y esteroides, fenoles, flavonoides y saponinas. Los volúmenes de droga administradas a los grupos de intervención con el binomio en la dosis de 0,7 mL manifestaron significativo aumento en el número de células granulosas sobre las células piramidales; a dosis de 3,5 mL el número de células granulosas fue menor con presencia de células piramidales grandes y pequeñas; y a dosis de 7,0 mL se manifestó desorganización celular, presencia de células piramidales grandes y pequeñas, y aumento de células granulosas. Conclusiones: El extracto alcohólico del binomio ayahuasca presenta efecto neuropatológico en el hipocampo del cerebro de ratas. (AU)

Observation of Localized States in Lieb Photonic Lattices.

We present the first experimental demonstration of a new type of localized state in the continuum, namely, compacton-like linear states in flat-band lattices. To this end, we employ photonic Lieb lattices, which exhibit three tight-binding bands, with one being perfectly flat. Discrete predictions are confirmed by realistic continuous numerical simulations as well as by direct experiments. Our results could be of great importance for fundamental physics as well as for various applications where light needs to be conducted in a diffractionless and localized manner over long distances.

Increased genomic prediction accuracy in wheat breeding through spatial adjustment of field trial data.

In crop breeding, the interest of predicting the performance of candidate cultivars in the field has increased due to recent advances in molecular breeding technologies. However, the complexity of the wheat genome presents some challenges for applying new technologies in molecular marker identification with next-generation sequencing. We applied genotyping-by-sequencing, a recently developed method to identify single-nucleotide polymorphisms, in the genomes of 384 wheat (Triticum aestivum) genotypes that were field tested under three different water regimes in Mediterranean climatic conditions: rain-fed only, mild water stress, and fully irrigated. We identified 102,324 single-nucleotide polymorphisms in these genotypes, and the phenotypic data were used to train and test genomic selection models intended to predict yield, thousand-kernel weight, number of kernels per spike, and heading date. Phenotypic data showed marked spatial variation. Therefore, different models were tested to correct the trends observed in the field. A mixed-model using moving-means as a covariate was found to best fit the data. When we applied the genomic selection models, the accuracy of predicted traits increased with spatial adjustment. Multiple genomic selection models were tested, and a Gaussian kernel model was determined to give the highest accuracy. The best predictions between environments were obtained when data from different years were used to train the model. Our results confirm that genotyping-by-sequencing is an effective tool to obtain genome-wide information for crops with complex genomes, that these data are efficient for predicting traits, and that correction of spatial variation is a crucial ingredient to increase prediction accuracy in genomic selection models.

Urban sprawl and fragmentation in Latin America: a dynamic quantification and characterization of spatial patterns.

South America is one of the most urbanized continents in the world, where almost 84% of the total population lives in cities, more urbanized than North America (82%) and Europe (73%). Spatial dynamics, their structure, main features, land consumption rates, spatial arrangement, fragmentation degrees and comparability, remain mostly unknown for most Latin American cities. Using satellite imagery the main parameters of sprawl are quantified for 10 Latin American cities over a period of 20 years by monitoring growth patterns and identifying spatial metrics to characterize urban development and sprawling features measured with GIS tools. This quantification contributes to a better understanding of urban form in Latin America. A pervasive spatial expansion has been observed, where most of the studied cities are expanding at fast rates with falling densities trend. Although important differences in the rates of land consumption and densities exist, there is an underlying fragmentation trend towards increasing sprawl. These trends of spatial discontinuity may eventually be intensified by further economic development. Urban Sprawl/Latin America/GIS metrics/spatial development.

Cardiopatías congénitas del adulto: algunas consideraciones sobre aspectos angiográficos / Congenital heart diseases in the adult, angiographic features

Esta experiencia, aunque pequeña, nos ha permitido orientar mejor el estudio hemodinámico y angiográfico de los adultos portadores de cardiopatías congénitas. Así cuando el paciente es evaluado por método invasivo, debe hacerse un examen completo que incluya, además del registro de presiones y oximetrías simultáneas, estudio angiográfico en la cavidad que sea necesaria con el fin de aclarar el diagnóstico principal y las patologías asociadas; utilizándose para ello las proyecciones axiales, en especial la utilización del septum. El no usar toda esta metodología puede significar dejar al Cirujano sin la completa información requerida para la adecuada corrección quirúrgica del paciente