Religious and spiritual support in the conception of nurses and families of critical patients: a cross-sectional study.

OBJECTIVE: To assess the religious and spiritual support in critical care according to the conception of patients' family members and nurses. METHOD: This is a quantitative cross-sectional study conducted with nurses and relatives of patients admitted to intensive care units. Sociodemographic data were collected and a questionnaire on religiosity and spirituality was applied to family members and the Spirituality and Spiritual Care Rating Scale to nurses. The results with p ≤ 0.05 were significant. RESULTS: Sample consisted of intensive care nurses (n = 22) and family members (n = 61). Intensive care nurses stated that they can provide spiritual care (p = 0.03). Of the family members, 88.5% believed that religiosity and spirituality help in coping with difficult times, highlighting prayer as part of care for critically ill patients (p = 0.02). CONCLUSION: Nurses and family members recognized religious/spiritual support as significant to the process of caring for critically ill patients, even though professional qualification is required for greater integration of this support in daily intensive care.

Religious and spiritual support in the conception of nurses and families of critical patients: a cross-sectional study / Soporte religioso y espiritual en la concepción de enfermeros y familiares de pacientes críticos: estudio transversal / Suporte religioso e espiritual na concepção de enfermeiros e familiares de pacientes críticos: estudo transversal

ABSTRACT Objective: To assess the religious and spiritual support in critical care according to the conception of patients' family members and nurses. Method: This is a quantitative cross-sectional study conducted with nurses and relatives of patients admitted to intensive care units. Sociodemographic data were collected and a questionnaire on religiosity and spirituality was applied to family members and the Spirituality and Spiritual Care Rating Scale to nurses. The results with p ≤ 0.05 were significant. Results: Sample consisted of intensive care nurses (n = 22) and family members (n = 61). Intensive care nurses stated that they can provide spiritual care (p = 0.03). Of the family members, 88.5% believed that religiosity and spirituality help in coping with difficult times, highlighting prayer as part of care for critically ill patients (p = 0.02). Conclusion: Nurses and family members recognized religious/spiritual support as significant to the process of caring for critically ill patients, even though professional qualification is required for greater integration of this support in daily intensive care.

RESUMEN Objetivo: Evaluar el soporte religioso y espiritual en cuidados criticos en la concepción de familiares de pacientes y enfermeros. Método: Estudio cuantitativo transversal realizado con enfermeros y familiares de pacientes hospitalizados en UCIs. Se recolectaron datos sociodemográficos y se realizó una encuesta sobre religiosidad y espiritualidad a los familiares y los enfermeros a Spirituality and Spiritual Care Rating Scale. Fueron significativos los resultados con p < 0,05. Resultados: Muestra compuesta de enfermeros intensivistas (n = 22) y familiares (n = 61). Los enfermeros intensivistas declararon que pueden ofrecer cuidado espiritual (p = 0,03). Entre los familiares, 88,5% creyeron que la religiosidad y la espiritualidad auxilian en el enfrentamiento de momentos dificiles, destacaron la oración como parte del cuidado en la asistencia al paciente critico (p = 0,02). Conclusión: Enfermeros y familiares reconocieron el soporte religioso/espiritual como significativo en el proceso de cuidado a los pacientes criticos, aunque a los enfermeros sea vigente la necesidad de calificación profesional para mayor integración de ese soporte en el cuidado intensivo diario.

RESUMO Objetivo: Avaliar o suporte religioso e espiritual em cuidados críticos na concepcäo de familiares de pacientes e enfermeiros. Método: Estudo quantitativo transversal realizado com enfermeiros e familiares de pacientes internados na terapia intensiva. Foram coletados dados sociodemográficos e aplicado questionário sobre religiosidade e espiritualidade para os familiares e aos enfermeiros a escala Spirituality and Spiritual Care Rating Scale. Foram significativos os resultados com p < 0,05. Resultados: Amostra composta de enfermeiros intensivistas (n = 22) e familiares (n = 61). Os enfermeiros intensivistas declararam que podem oferecer cuidado espiritual (p = 0,03). Dos familiares, 88,5% acreditaram que a religiosidade e a espiritualidade auxiliam no enfrentamento de momentos difíceis, destacando a oração como parte do cuidado na assisténcia ao paciente crítico (p = 0,02). Conclusão: Enfermeiros e familiares reconheceram o suporte religioso/espiritual como significativo ao processo de cuidar dos pacientes críticos, ainda que para os enfermeiros seja vigente a necessidade de qualificação profissional para maior integração desse suporte no cuidado intensivo diário.

Tuberculose latente entre pessoas com HIV/AIDS / Latent tuberculosis among people with HIV/AIDS

Objetivo: buscar evidências sobre o uso da terapia preventiva com isoniazida na redução do risco da tuberculose ativa entre as pessoas vivendo com HIV/Aids mediante os resultados dos ensaios de liberação do interferon-gama (IGRA). Método: trata-se de estudo bibliográfico, descritivo, tipo revisão integrativa, com busca de artigos nas bases de dados publicados de 2010 a 2017. Os descritores utilizados foram HIV, AIDS, terapia preventiva com isoniazida, tratamento da infecção latente da tuberculose, interferon-gama, ensaios de liberação do interferon-gama. Resultados: selecionaram-se três artigos, com 1815 PVHA e desenvolvimento de tuberculose em apenas um caso, após 24 meses de terapia preventiva com isoniazida. Altas taxas de reversão e conversão durante o seguimento. Conclusão: consideraram-se os estudos abordando os benefícios da terapia preventiva com isoniazida a partir do resultado do IGRA apresentam-se escassos, com incipiente sugestão da limitação do teste seriado para o seguimento da resposta da terapia entre as PVHA. Além disso, destaca-se a necessidade de se investir em mais estudos que explorem a temática e superem as limitações já encontradas tendo em vista a alta relevância de se investir em novas estratégias que impactem o controle da coinfecção TB/HIV.(AU)

Flow cytometry of human primary epidermal and follicular keratinocytes.

OBJECTIVE: The aim of this study was to characterize using flow cytometry cultured human primary keratinocytes isolated from the epidermis and hair follicles by different methods. METHODS: Human keratinocytes derived from discarded fragments of total skin and scalp hair follicles from patients who underwent plastic surgery in the Plastic Surgery Division at UNIFESP were used. The epidermal keratinocytes were isolated by using 3 different methods: the standard method, upon exposure to trypsin for 30 minutes; the second, by treatment with dispase for 18 hours and with trypsin for 10 minutes; and the third, by treatment with dispase for 18 hours and with trypsin for 30 minutes. Follicular keratinocytes were isolated using the standard method. RESULTS: On comparing the group treated with dispase for 18 hours and with trypsin for 10 minutes with the group treated with dispase for 18 hours and with trypsin for 30 minutes, it was observed that the first group presented the largest number of viable cells, the smallest number of cells in late apoptosis and necrosis with statistical significance, and no difference in apoptosis. When we compared the group treated with dispase for 18 hours and with trypsin for 10 minutes with the group treated with trypsin, the first group presented the largest number of viable cells, the smallest number of cells in apoptosis with statistical significance, and no difference in late apoptosis and necrosis. When we compared the results of the group treated with dispase for 18 hours and with trypsin for 10 minutes with the results for follical isolation, there was a statistical difference in apoptosis and viable cells. CONCLUSION: The isolation method of treatment with dispase for 18 hours and with trypsin for 10 minutes produced the largest number of viable cells and the smallest number of cells in apoptosis/necrosis.