ABSTRACT
Tibial dyschondroplasia (TD) is a disease of many avian species characterized by an enlarged and avascular lesion in the proximal tibiotarsal bone. The aim of present study was to evaluate the effects of hypoxia-inducible factor-1α(HIF-1α) inhibition on thiram- induced TD using synthetic medicine FK228 and the association between HIF-1α and heat-shock protein 90 (Hsp90). One hundred and fifty broiler chicks were equally divided into 3 groups: control; thiram fed; and FK228 treatment. Expressions of HIF-1α and Hsp90 genes were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR) on day 10 and 14 post-hatch. Western blot analysis of HIF-1α and Hsp90 gene was performed to measure the protein levels at the end of the experiment. Results showed that HIF-1α and Hsp90 levels were significantly (P less than 0.05) up-regulated in the thiram group as compared to the control group. Meanwhile, FK228 (HIF-1α inhibitor) significantly (P less than 0.05) down- regulated the mRNA and protein levels of HIF-1α and Hsp90, restored the size of growth plate and diminished lameness. In conclusion, HIF-1α and Hsp90 play an important role in the formation of avascular growth plate and there is a direct relationship between HIF-1α and Hsp90 for the progression of TD pathogenesis. Therefore, HIF- 1α may prevent and control TD in broiler chickens.
Subject(s)
Avian Proteins/metabolism , Bone Diseases , Depsipeptides/pharmacology , Hypoxia-Inducible Factor 1, alpha Subunit/metabolism , Poultry Diseases , Thiram/toxicity , Tibia/metabolism , Animals , Bone Diseases/chemically induced , Bone Diseases/drug therapy , Bone Diseases/metabolism , Bone Diseases/pathology , Chickens , Growth Plate/metabolism , Growth Plate/pathology , Poultry Diseases/chemically induced , Poultry Diseases/drug therapy , Poultry Diseases/metabolism , Poultry Diseases/pathology , Tibia/pathologyABSTRACT
AIM: To use a newly developed radiographic technique to compare apical transportation and loss of working length (WL) between.06 taper ProFile Series 29 and ProTaper nickel-titanium (NiTi) rotary instruments in vitro. METHODOLOGY: Mesio-buccal canals of 40 extracted mandibular molars were randomly divided into two groups. Group 1 was instrumented with ProFile and group 2 with ProTaper instruments according to the manufacturers' directions. A specially constructed radiographic jig with a Schick digital radiographic system (Schick Technologies Inc., Long Island City, NY, USA) was used to take pre- and postoperative radiographs of the samples at predetermined angulations. Using AutoCAD 2000 (Autodesk Inc., San Rafael, CA, USA), the central axes of initial and final instruments were radiographically superimposed to determine the loss of WL and degree of transportation at D(0), D(1), D(2) and D(4) from the WL. Data were analysed using repeated-measures anova. RESULTS: A statistically significant difference in apical transportation was found at the D(4) level between the two groups (P = 0.05). There was no statistical significance regarding postinstrumentation change in WL between groups. Spearman's Bivariate Correlation analysis indicated no statistically significant relationship between the radius of curvature and transportation. CONCLUSION: The results indicate that both ProTaper and ProFile instruments are comparable to each other in regards to their ability to optimally enlarge root canal with minimal transportation and loss of WL in vitro.
Subject(s)
Dental Instruments , Dental Pulp Cavity/diagnostic imaging , Root Canal Preparation/instrumentation , Tooth Apex/diagnostic imaging , Dental Alloys , Dental Pulp Cavity/anatomy & histology , Dental Stress Analysis , Equipment Design , Humans , Molar , Nickel , Radiographic Image Interpretation, Computer-Assisted , Titanium , Tooth Apex/anatomy & histologyABSTRACT
This study histologically assessed the effect of enamel matrix derivative (EMDOGAIN) on periodontal healing in re-implanted teeth in nine Beagle dogs. Incisors in dogs were re-implanted after 15, 30 and 60 min of dry storage with and without the application of EMDOGAIN. The pulps were removed in all teeth. In groups 1 and 2, dog teeth were splinted and the dogs sacrificed after 8 and 12 weeks, respectively. In group 3, dog teeth were not splinted and sacrifice took place after 12 weeks. The histological parameters studied were healed PDL, surface, inflammatory and replacement resorption. Univariable and multivariable analysis of data was performed. Increased incidence of healed PDL was inversely proportional to extraalveolar time. No significant differences were noticed between splinted and non-splinted teeth. The EMDOGAIN group showed a higher incidence of healed PDL, while controls showed a higher incidence of ankylosis. The effect of EMDOGAIN was more pronounced at the 12-week interval.
Subject(s)
Dental Enamel Proteins/therapeutic use , Incisor/injuries , Periodontal Ligament/drug effects , Tooth Avulsion/surgery , Tooth Replantation , Analysis of Variance , Animals , Ankylosis/etiology , Ankylosis/pathology , Cell Division/drug effects , Dogs , Follow-Up Studies , Incidence , Incisor/surgery , Multivariate Analysis , Observer Variation , Periodontal Ligament/physiopathology , Periodontitis/etiology , Periodontitis/pathology , Regeneration/drug effects , Reproducibility of Results , Root Canal Therapy , Root Resorption/etiology , Root Resorption/pathology , Splints , Time Factors , Tooth Avulsion/physiopathology , Tooth Socket/pathology , Wound Healing/drug effectsABSTRACT
The effect of the application of a matrix band and the use of cavity varnish on the microleakage of Cavit in endodontic access preparations was investigated. Sixty human upper premolars were divided into six groups of ten specimens each. Access cavities with proximal extensions were prepared and filled with 1, Cavit only; 2, Cavit after application of varnish; 3, Cavit with the use of a matrix band; 4, Cavit with a matrix band and varnish; 5, incremental deposition of Cavit with the use of a matrix band and varnish; and 6, gutta percha stopping. The specimens were placed in 2% methylene blue dye, thermocycled, and evaluated for both proximal and occlusal linear leakage. The experimental group in which Cavit was incrementally deposited in varnish-lined and matrix-supported cavities exhibited significantly (p < 0.05) less proximal microleakage than those groups in which cavities were filled without the application of both a matrix band and a cavity varnish.
