ABSTRACT
SUBESCO is an audio-only emotional speech corpus for Bangla language. The total duration of the corpus is in excess of 7 hours containing 7000 utterances, and it is the largest emotional speech corpus available for this language. Twenty native speakers participated in the gender-balanced set, each recording of 10 sentences simulating seven targeted emotions. Fifty university students participated in the evaluation of this corpus. Each audio clip of this corpus, except those of Disgust emotion, was validated four times by male and female raters. Raw hit rates and unbiased rates were calculated producing scores above chance level of responses. Overall recognition rate was reported to be above 70% for human perception tests. Kappa statistics and intra-class correlation coefficient scores indicated high-level of inter-rater reliability and consistency of this corpus evaluation. SUBESCO is an Open Access database, licensed under Creative Common Attribution 4.0 International, and can be downloaded free of charge from the web link: https://doi.org/10.5281/zenodo.4526477.
Subject(s)
Speech/classification , Adult , Bangladesh , Emotions , Female , Humans , India , Language , Male , Recognition, Psychology , Reproducibility of Results , Speech Perception , Verbal BehaviorABSTRACT
A highly sensitive enzyme immunoassay is described for the detection of atrazine residues in water. Atrazine derivative was conjugated to Bovine Serum Albumin (BSA) to obtain an immunizing antigen and to Horseradish Peroxidase enzyme (POD) to obtain a marker for immunoassay. The formation of these conjugations was confirmed by UV spectroscopy as well as by gel-electrophoresis. Polyclonal antibodies were raised in rabbits by immunization with an atrazine-BSA conjugate containing 29 atrazine residues per BSA molecule. An ELISA on microtitration plates was optimized with peroxidase-atrazine conjugate. The middle of the test (50% B/Bo) was found to be at 90 ng/l, which is well below the maximum concentration permitted by the EC guidelines for drinking water. Detection limits for atrazine of about 1 ng/l could be reached. The assay did not require concentration or cleanup steps for drinking or ground water samples. Validation experiments showed good accuracy and precision. No cross-reactivities were shown by other s-triazines like terbutryn, ametryn, terbuthylazine, des-isopropylatrazine, and de-ethylatrazine except hydroxyatrazine. The latter was present at very low levels that can be calibrated/standardized before analysis or it may be considered as leftover residues of atrazine. Based on these results, it is suggested that this test can be applied to obtain fairly accurate results for atrazine concentration in water samples from different sources.
