ABSTRACT
Water-soluble arsenic compound fractions were extracted from seven species of jellyfishes and subjected to analysis by high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) for arsenicals. A low content of arsenic was found to be the characteristic of jellyfish. Arsenobetaine (AB) was the major arsenic compound without exception in the tissues of the jellyfish species and mucus-blobs collected from some of them. Although the arsenic content in Beroe cucumis, which preys on Bolinopsis mikado, was more than 13 times that in B. mikado, the chromatograms of these two species were similar in the distribution pattern of arsenicals. The nine species of jellyfishes including two species treated in the previous paper can be classified into arsenocholine (AC)-rich and AC-poor species. Jellyfishes belonging to Semaostamae were classified as AC-rich species.
Subject(s)
Arsenicals/pharmacokinetics , Scyphozoa/chemistry , Water Pollutants, Chemical/pharmacokinetics , Animals , Arsenicals/analysis , Chromatography, High Pressure Liquid , Food Chain , Mass Spectrometry , Mucus/chemistry , Tissue Distribution , Water Pollutants, Chemical/analysisABSTRACT
Concentrations of the 18 elements, barium (Ba), beryllium (Be), bismuth (Bi), calcium (Ca), cadmium (Cd), cesium (Cs), copper (Cu), lanthanum (La), lithium (Li), magnesium (Mg), molybdenum (Mo), lead (Pb), rubidium (Rb), antimony (Sb), tin (Sn), strontium (Sr), thallium (Tl), and zinc (Zn), were determined in the synovial fluids of osteoarthritic knee joints and in the corresponding sera of 16 patients by inductively coupled plasma-mass spectrometry. Knee-joint effusions have lower elemental concentrations than their corresponding sera. For the essential elements Ca, Cu, Mg, and Zn and for the nonessential and toxic elements Ba, Be, Bi, La, and Sb, this difference was highly significant. Strong positive correlations between concentrations in effusions and sera for the essential elements Cu and Mg and for the nonessential elements Cs, Li, Rb, and Sr could be established. The grade of localized hyperperfusion of the knee region in the blood pool phase of 99mTc HDP bone scan indicating inflammation did not correlate with any elemental concentration determined.
Subject(s)
Osteoarthritis, Knee/metabolism , Trace Elements/analysis , Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Quality Control , Spectrophotometry, Atomic , Synovial Fluid/chemistryABSTRACT
When mercury is quantified by ICP-MS under routine conditions (external calibration) in reference materials, which require mineralization with nitric acid, the experimental concentrations are almost always unacceptably low in comparison to certified values. Sorption of mercury on the Teflon surfaces of the digestion vessels, changes in the viscosity of the aspirated solutions, in the efficiency of the nebulization, in the aerosol transport, and memory effects cannot be responsible for the low results. The intensity of a mercury signal is strongly dependent on the concentration of nitric acid (and other mineral acids) in the measured solutions. Correct results for mercury in the SRM GBW-90101 (Chinese human hair; 2.16+/-0.21 mg Hg/kg certified) can only be obtained, when the solutions, with which the external calibration curves were established, have exactly the same nitric acid concentration as the aspirated digests (2.03+/-0.01 mg Hg/kg; n = 5), when mercury is determined by the standard addition method (2.10+/-0.01 mg Hg/kg; n = 5), or when the experimental mercury concentration obtained at a nitric acid concentration in the digest, different from the concentration in the external calibration solutions, is corrected mathematically based on a pre-established function [Hg2+] = f [HNO3]. The concentrations found by this mathematically based correction 2.04+/-0.01 mg Hg/kg (n = 5) is in good agreement with the values obtained by acid matched calibration or by the standard addition method. For practical work with large numbers of samples the mathematical correction appears to be the method of choice. For occasional mercury determinations, the standard addition method seems to be the most practicable.
Subject(s)
Hair/chemistry , Mercury/analysis , China , Humans , Mass Spectrometry , Microwaves , Nitric Acid/chemistry , Polytetrafluoroethylene/chemistry , Reference Standards , Reproducibility of Results , Spectrophotometry, Atomic , Time FactorsABSTRACT
The retention behavior of four naturally occurring dimethylarsinoylribosides with -CH2-CHOH-CH2X (X = OH, HO3POCH2CHOHCH2OH, SO3H, OSO3H) as aglycones, of arsenous acid, arsenic acid, methylarsonic acid, and dimethylarsinic acid was investigated on a Hamilton PRP-X100 anion-exchange column with aqueous solutions of ammonium dihydrogen phosphate (20 mmol/L) in the pH range of 3.8-9.0 as mobile phase. A HP 4500 inductively coupled plasma mass spectrometer (ICP-MS) served as arsenic-specific detector. The influence of pH, temperature, and the concentration of methanol in the mobile phase on the retention times of these arsenic compounds was explored. An aqueous 20 mM ammonium dihydrogen phosphate solution at pH 5.6 at a column temperature of 40 degrees C was considered optimal as it allowed the separation of seven of the arsenic compounds within 16 min. Only arsenous acid and the ribose with the glycerol aglycone have overlapping signals with both migrating almost with the solvent front. At a concentration of 0.50 ng As mL(-1) the relative standard deviations (n = 3) of the signal areas of the eight arsenic compounds was in the range from 3.5 to 8.1%. The linear calibration curves (peak areas) from 0.5 to 10 ng/mL had correlation coefficients > 0.997. Extracts obtained from the brown algae Fucus spiralis and Halidrys siliquosa were chromatographed under the optimized conditions. Both species contained the sulfate riboside as the major arsenic compound (approximately 55% of total extractable arsenic) together with the sulfonate- and phosphate riboside. Arsenic acid was a significant constituent of Halidrys siliquosa (approximately 6.5%), but was not detected in Fucus spiralis.
Subject(s)
Arsenicals/analysis , Phaeophyceae/chemistry , Arsenicals/chemistry , Chromatography, Ion Exchange/methods , Mass Spectrometry/methods , Sensitivity and SpecificityABSTRACT
A microwave procedure for the digestion of the NIST 1634b reference material "residual fuel oil" in closed pressurized vessels was developed in an attempt to facilitate routine analysis and obtain reproducible conditions or comparable results. The influence of sample size, reagent composition and volume, microwave power, and duration of heating on the digestion procedure was studied. Pressure and temperature inside the reaction vessels were monitored to determine the progression of the reaction and to develop optimal conditions. A nine-step heating program requiring 36.5 min with microwave power not exceeding 450 W in the pulsed mode was found suitable for the digestion of approximately 250 mg fuel oil with a mixture of nitric acid (5.0 mL) and hydrogen peroxide (2.0 mL). The reproducibility of microwave power was determined in terms of the relative standard deviations (n = 3) for temperature (2.7%) and pressure (4.9%) data. The vapor pressures obtained with 5.0 mL Milli-Q water (heated) in an 80-mL digestion vessel showed good agreement with literature data. The excess acid in the resulting digests was removed by evaporation and the concentrations of 24 elements (Ag, Al, As, Ba, Bi, Ca, Cd, Co, Cr, Cu, Fe, Hg, Mg, Mo, Ni, Pb, Sb, Sn, Sr, Ti, Tl, V, U, and Zn) were determined in the diluted digests by inductively coupled plasma mass spectrometry (ICP-MS). The experimental results were in good agreement with the certified and recommended concentrations for eight elements (Al, As, Co, Cr, Ni, Pb, V, Zn) in solutions obtained after one digestion step. An additional digestion step, consisting of intermediate cooling and venting stages, was required for the accurate determination of Fe. No agreement was reached for Ca and Ba even after two-step digestion. The proposed method of digestion provided precise results with relative standard deviations generally less than 5% for most of the elements determined.
ABSTRACT
In pleural effusions and sera from 66 patients copper and zinc were quantified by inductively coupled argon plasma-mass spectrometry after mineralizations in a closed-pressurized microwave unit with a mixture of concentrated nitric acid and 30% hydrogen peroxide. Total protein, pH, leukocyte count, lactate dehydrogenase, glucose, C-reactive protein, ceruloplasmin, and alpha1-antitrypsin were determined in many of the effusions. All but four effusions had concentrations of copper (range 58-1720 microg/kg) and zinc (range 27-1001 microg/kg) that were lower than the concentrations in the corresponding sera. Very high concentrations of zinc (1930-6470 microg/kg) were characteristic for thoracic empyemata. In the scatterplots of serum copper versus effusion copper, serum zinc versus effusion zinc, and serum copper/effusion copper versus serum zinc/effusion zinc no clearly delineated regions were noticeably useful for identifying malignant effusions. Similar plots of the concentrations of copper or zinc versus the eight clinical laboratory parameters or plots of clinical parameter versus clinical parameter failed to be of diagnostic value. Statistically highly significant correlations (p < or = 0.05, n > 45, r2 > 0.25) were observed for 9 of 28 pairs of the clinical parameters, for total protein and copper in the effusions and zinc in the effusions and for ceruloplasmin and copper in the effusions. Among the patients suffering from benign or malignant effusions, 52% had zinc concentrations in the sera below the low limit of the normal range (600 microg/kg). Supplementation of such patients with zinc should be considered.
Subject(s)
Copper/metabolism , Neoplasms/metabolism , Pleural Effusion/metabolism , Zinc/metabolism , Adolescent , Adult , Aged , Ceruloplasmin/metabolism , Copper/blood , Female , Humans , Male , Mass Spectrometry/methods , Middle Aged , Neoplasms/blood , Pleural Effusion, Malignant/metabolism , Predictive Value of Tests , Zinc/bloodABSTRACT
In this study, a number of selected trace elements and clinically relevant parameters were compared between thoracic empyemata and the corresponding sera for a better understanding of the trace element distribution between these two compartments. Serumempyema pairs were obtained from 13 patients and quantified for selected and essential trace elements, namely copper (Cu), zinc (Zn), manganese (Mn), rubidium (Rb), and magnesium (Mg), by inductively coupled plasma-mass spectrometry (ICP-MS). In addition, the concentrations of the following clinical laboratory parameters were analyzed by standard methods: total protein, leukocyte count, lactate dehydrogenase, glucose, pH, and the C-reactive protein. Individual concentrations of the elements determined in the empyemata were frequently higher than in pleural effusions of any other benign or malignant condition except for Cu. Serum Cu exceeded the normal range (600-1400 microg/kg) in 6 out of 13 patients (median 1410 microg/kg). In the empyemata, Zn concentrations (median 2000 microg/kg) were characteristically higher than in the sera (median 450 microg/kg) and exceeded the upper limit for serum (1200 microg/kg) in 8 of the 13 patients. Manganese concentrations in the empyemata (median 2.7 microg/kg) were also higher compared to corresponding sera, although they stayed within the limits considered normal for serum of healthy adults (upper limit 2.9 microg/kg). Rubidium was also moderately higher in most empyemata (median 290 microg/kg) and exceeded the upper limit for serum (560 microg/kg) in two patients. The median concentration of the essential element magnesium was higher in the empyemata (23 mg/kg) than in the sera (21 mg/kg). However, all serum Mg concentrations except three remained within the normal range (17-22 mg/kg). Removal of large amounts of empyematous fluid may deprive the body of trace elements and can cause suboptimal or deficient trace element status and homeostasis. Recuperation will be accelerated by compensatory supplementation of trace elements. Therefore, selective medication with adequate trace element compounds in patients with thoracic empyema can be generally recommended for zinc. The other elements need not necessarily be monitored or substituted, because of their stable concentrations in the serum. Rb may have a biological impact, but deficiency symptoms in man are not clearly defined.
Subject(s)
Empyema, Pleural/metabolism , Trace Elements/metabolism , Aged , Aged, 80 and over , Copper/metabolism , Empyema, Pleural/blood , Female , Humans , Hydrogen-Ion Concentration , L-Lactate Dehydrogenase/blood , Magnesium/metabolism , Male , Manganese/metabolism , Middle Aged , Pleural Effusion/metabolism , Rubidium/metabolism , Trace Elements/blood , Zinc/metabolismABSTRACT
A technique of hydride cold-trapping atomic absorption spectrometry following microwave digestion was developed and optimized for the determination of selenium in human milk. The method was validated by the analysis of two standard reference materials (CRM milk powder). The detection limit was 0.5 ng mL(-)(1). The method was then used to analyze 78 milk samples from 38 Austrian mothers throughout their first 10 months of lactation. The mean concentration of selenium in the mother's milk decreased with the days postpartum from 23.9 +/- 12.0 microg L(-)(1) in colostrum to a plateau of 11.4 +/- 3.0 microg L(-)(1) in mature milk. On the basis of the milk selenium concentrations, the selenium intakes of the fully breast-fed infants and the lactating mothers were calculated. The selenium intake of the infants during their first 3 months of life was >8.2 microg day(-)(1). The selenium intake of the lactating mothers was 48 microg day(-)(1). Compared to the recommended dietary allowance, the fully breast-fed infants received sufficient selenium but the lactating mothers obtained less than the recommended.
Subject(s)
Breast Feeding , Diet , Milk, Human/chemistry , Selenium/analysis , Austria , Female , Humans , Infant , Infant, Newborn , Lactation , Postpartum Period , Spectrophotometry, Atomic/methodsABSTRACT
The advantages accruing to biochemical and clinical investigations from a method that allows the simultaneous quantification (RSD < or = 10%) of many elements in blood, plasma, and serum at concentrations equal to one-hundredth of the lower limits of the normal ranges are undeniable. The suitability of inductively coupled argon plasma low-resolution quadrupole mass spectrometry (ICP-MS), a simultaneous method with low detection limits, is evaluated for the quantification of inorganic constituents in whole blood, plasma, and serum with consideration of the dilution associated with the mineralization of the samples, of isobaric and polyatomic interferences and of normal ranges. Of the 3 bulk elements, the 3 major electrolytes, the 15 essential elements, the 8 toxic elements, the 4 therapeutic elements, and the 14 elements of potential interest (total of 47 elements) only 7 elements (Ca, Cu, K, Mg, Rb, Sr, Zn) can be simultaneously quantified under these rigorous conditions in serum and only 8 elements (additional element Pb) in whole blood. Quantification of elements in the Seronorm Standards "Whole Blood" and "Serum" showed, that this list of simultaneously determinable elements in these matrices is reasonable. Although this list is disappointingly short, the number of elements determinable simultaneously by ICP-MS is still larger than that by ICP-AES or GFAAS. Improved detectors, more efficient nebulizers, avoidance of interferences, better instrument design, and high-resolution mass spectrometers promise to increase the number of elements that can be determined simultaneously.
Subject(s)
Mass Spectrometry/methods , Trace Elements/blood , Humans , Reference Standards , Sensitivity and SpecificityABSTRACT
Mussels (Mytilus edulis) were exposed to trimethyl(carboxymethyl)arsonium bromide (arsenobetaine, C-1 betaine), trimethyl(2-carboxyethyl)arsonium bromide (C-2 betaine), or trimethyl(3-carboxypropyl)arsonium bromide (C-3 betaine). Arsenic was accumulated by the mussels in all cases but the efficiency of uptake decreased with the number of methylene units in the carboxyalkyl group. Arsenobetaine (C-1 betaine) was the most readily accumulated, followed by the C-2 betaine (70% as efficient as arsenobetaine) and the C-3 betaine (approximately 7%). Chromatographic analysis (HPLC-ICPMS) of extracts of the mussels demonstrated that the arsenic compounds were accumulated unchanged. A 46-day depuration period which followed exposure did not significantly reduce the arsenic concentration in any of the three groups. Comparison with previous data on accumulation of arsenic compounds by M. edulis indicates that uptake may be influenced by the presence of a quaternary arsonium group and the zwitterionic nature of the arsenic-betaines.
Subject(s)
Arsenicals/metabolism , Bivalvia/metabolism , Animals , Arsenicals/chemistry , Chromatography, High Pressure Liquid , Mass Spectrometry , Methylation , Structure-Activity RelationshipABSTRACT
A speciation method was developed for selenious acid, selenic acid, trimethylselenonium ion (TMSe) and selenomethionine (SeMet). Separation of the four selenium species was achieved by HPLC on an ESA Anion III anion-exchange column using aqueous mobile phase of 5.5 mmol/L ammonium citrate at pH 5.5 with a flow rate of 1.5 mL/min. Under the optimal conditions, the four selenium species were separated within 8 minutes. On-line selenium-specific detection was carried out with an inductively coupled plasma mass spectrometer (ICP-MS) or a flame atomic absorption spectrometer (FAAS). The detection limits of HPLC-FAAS were approximately rho(Se) = 1 mg/L for each compound (100 microL injection). To increase the nebulization efficiency of the ICP-MS, the Meinhard concentric nebulizer was replaced by an ultrasonic nebulizer (USN). The ICP-MS signal intensity was increased by a factor of 7 for selenious acid and 24 to 31 for TMSe, SeMet and selenic acid with the USN compared to that with the Meinhard nebulizer. The detection limits of the HPLC-USN-ICP-MS were rho(Se) = 0.08 microgram/L for TMSe, rho(Se) = 0.34 microgram/L for selenious acid, rho(Se) = 0.18 microgram/L for SeMet and rho(Se) = 0.07 microgram/L for selenic acid.
Subject(s)
Selenium Compounds/analysis , Chromatography, High Pressure Liquid , Mass Spectrometry , Selenium Compounds/chemistry , Spectrophotometry, AtomicABSTRACT
Inductively coupled plasma mass spectrometry (ICP-MS) was used to determine 18 trace elements (Ba, Be, Bi, Cd, Co, Cs, Cu, La, Li, Mn, Mo, Pb, Rb, Sb, Sn, Sr, Tl, and Zn) in 55 human milk samples from 46 healthy mothers collected during lactation periods extending to 293 days after birth. Se was quantified by hydride generation atomic absorption spectrometry (HG-AAS). To test the accuracy and the precision of the analytical procedure, milk powder reference materials (BCR 063 and BCR 150) were analyzed. The results obtained by ICP-MS and HG-AAS showed good agreement with the certified values. Whenever available, trace element concentrations determined in the human milk samples were compared to reliable literature data. The concentrations of Be (< 0.05 to 0.9 microgram/kg), Bi (< 0.09 to 2.0 micrograms/kg), Cs (1.7 to 7.7 micrograms/kg), La (< 0.05 to 3.7 micrograms/kg), Rb (440 to 1,620 micrograms/kg), and Tl (< 0.08 to 0.5 microgram/kg) are the first to be reported for human milk. The concentrations of the essential trace elements Cu (p < 0.005), Mn (p < 0.05), Mo (p < 0.0005), Se (p < 0.001), and Zn (p < 0.0005) significantly decreased and the concentrations of cobalt significantly increased (p < 0.005) in human milk during the course of lactation. All concentrations for the essential trace element tin in the human milk samples were below the method detection limit of 0.3 microgram/kg. Among the not essential and toxic elements-with the exception of Ba, Pb, and Tl-the trend toward lower concentrations with continuing lactation is much less pronounced than for the essential trace elements. With the exception of Se, the daily intakes of essential trace elements of fully breast-fed infants are considerably lower than dietary recommendations.
Subject(s)
Milk, Human/chemistry , Trace Elements/analysis , Female , Humans , Infant , Lactation , Mass Spectrometry/methods , Quality ControlABSTRACT
1. The aerobic filamentous fungus S. brevicaulis IMI 17297 methylated antimony from Sb2O3 substrate, with the formation of gaseous trimethylantimony (TMA). No evidence was found for the generation of other gaseous antimony compounds by this organism. 2. Biovolatilization of inorganic antimony was greatest during cultivation of the fungus on solid media at 25 degrees C, and occurred more readily from antimony (III) substrates than from antimony (V) substrates. 3. Under simulated cot environment conditions (CO2 enriched atmosphere, 33 degrees C) the fungus exhibited an altered morphology and a reduced capability to volatilize inorganic antimony from the pure compound. 4. No evidence of antimony biovolatilization from cot mattress PVC was found, unless antimony was released from PVC by heat treatment (at 80 or 100 degrees C). 5. These data suggest that normal cot environment conditions are non-optimal for volatilization of antimony by S. brevicaulis, and that Sb2O3 in cot mattress PVC is not bioavailable. 6. Cot mattress isolates of S. brevicaulis also volatilized antimony (not encapsulated by PVC), whereas those of other filamentous fungi (Penicillium spp., Aspergillus niger, Aspergillus fumigatus, Alternaria sp.) and of bacteria (Bacillus spp.) did not. 7. The oxidation products of TMA may be the true determinants of toxicity for biogenic antimony gases produced in an aerobic environment.
Subject(s)
Antimony/metabolism , Infant Equipment/microbiology , Mitosporic Fungi/metabolism , Organometallic Compounds , Sudden Infant Death/etiology , Antimony/adverse effects , Antimony/analysis , Antimony/chemistry , Beds , Biodegradation, Environmental , Culture Media , Gas Chromatography-Mass Spectrometry , Humans , Infant, Newborn , Methylation , Polyvinyl Chloride/chemistry , VolatilizationABSTRACT
1. Polyvinyl chloride (PVC) cot mattress covers from SIDS cases were investigated as potential sources of soluble (potentially ingestable) antimony in the cot environment. 2. Body fluids (urine, saliva) and proprietary domestic detergents/sterilizing fluids markedly enhanced leaching of antimony from PVC. Release of antimony was also enhanced at both low and high pH and by elevated temperature. The extent of antimony leaching did not correlate well with PVC content of this element. 3. These data do not support the assumption that postmortem analysis of antimony content proves exposure to gaseous antimony trihydride from mattress PVC. 4. Ingestion of antimony released from PVC could account for the high variability associated with reported detectable levels of antimony in liver from both SIDS and other infants. It could also explain suspected additional postnatal exposure to this element, which gives rise to elevated levels of Sb in the hair of some healthy infants.
Subject(s)
Antimony/analysis , Infant Equipment , Polyvinyl Chloride/analysis , Sudden Infant Death/etiology , Antimony/adverse effects , Hair/chemistry , Humans , Hydrogen-Ion Concentration , Infant, Newborn , Liver/chemistry , Risk Factors , Saliva/chemistryABSTRACT
First void urine samples were collected from 24 residents in an area of past intense mining and smelting activity of arsenical ores. Seven samples were also taken from a control village. The arsenic species in the urine were separated and quantified with an HPLC-ICP-MS system equipped with a hydraulic high-pressure nebulizer. The detection limit for arsenic in urine using this system is 0.05 microgram dm-3. Creatinine was also determined for all samples to remove the influence of urine density and all results were expressed in microgram As g-1 creatinine. The results showed elevated levels of both organic and inorganic arsenic compounds in the 'exposed' population's urine when compared with those of the control group. The total As concentrations (less arsenobetaine) in the 'exposed' population were in the range 2.7-58.9 micrograms g-1 creatinine (mean 13.4, median 9.2 micrograms g-1) compared with the control group data range 2.5-5.3 micrograms g-1 (mean 4.2, median 4.7 micrograms g-1).
Subject(s)
Arsenic/urine , Environmental Exposure , Mining , Adolescent , Adult , Aged , Arsenates/urine , Arsenicals/urine , Arsenites/urine , Cacodylic Acid/urine , Child , Child, Preschool , Creatinine/urine , England , Female , Humans , Male , Middle Aged , Pilot Projects , Teratogens/metabolismABSTRACT
With inductively coupled plasma-mass spectrometry (ICP-MS), the 18 trace elements Ba, (Be), (Bi), Cd, Co, Cs, Cu, La, Li, Mn, Mo, Pb, Rb, (Sb), (Sn), Sr, (Tl), and Zn were quantified in the digests of 13 formulas based on cow milk, of two formulas based on soy protein, of two milk powders, from which formulas were prepared, of two samples of Austrian cow milk, and in the water, with which the powders were suspended. Concentrations in parentheses were at or below the method detection limits in the formulas. The accuracy and precision of the analytical procedure tested with milk powder reference materials BCR 063 and BCR 150 were satisfactory. The concentrations of trace elements in the powders vary considerably from batch to batch. The ratios of high to low concentrations ranged from 1.1 to 4.8 and were higher for the essential trace elements Co, Cu, Mn, Mo, Sn, and Zn than for nonessential or toxic elements. The contribution of tap water from the water system of the city of Graz, Austria to the concentrations of trace elements in the formulas ranges from 45% for Pb to 0.2% for Rb and is negligible, for instance, for Cd, Cs, La, Mo, and Sn. Preformulas and follow-up formulas are partly supplemented with the essential trace elements Cu, Mn, and Zn and, therefore, concentrations of these trace elements in the formulas vary considerably. However, supplementation of a formula with a particular element must not necessarily result in higher concentrations compared to non-supplemented formulas. Concentrations of the essential elements were in the following ranges for preformulas, follow-up formulas, soy-based formulas (in microg/kg): Co, 8.3-11.2, 4.5-13, 5.0-5.7; Cu, 330-750, 27-730, 440-530; Mn, 33-580, 40-390, 440-530; Mo, 10-32, 9-39, 44-46; Sn, <0.44-3.8, <0.44-1.0, <0.44-5.8; Zn, 3340-11,380, 4120-7100, 5590-6,840. A preformula supplemented with Mn had a 10 times higher manganese concentration than preformulas without supplementation. Concentrations of all trace elements quantified were lower in cow milk than in formulas and do not meet the dietary requirements of infants.
Subject(s)
Infant Food/analysis , Mass Spectrometry/methods , Milk/chemistry , Trace Elements/analysis , Animals , Cattle , Quality Control , Reproducibility of Results , Sensitivity and Specificity , Glycine max/chemistryABSTRACT
The trace elements Ba, Bi, Cd, Co, Cs, Cu, Hg, La, Mn, Mo, Pb, Rb, Sb, Sn, Sr, Tl, and Zn were determined by inductively coupled plasma mass spectrometry in plasma samples of 68 hemodialysis patients. The same elements (with exception of La and Mn) were also determined in whole blood after mineralization with high-purity nitric acid/hydrogen peroxide in a closed-pressurized microwave system. The accuracy and precision was checked by analyzing two Seronorm "whole blood" reference materials. All samples were contaminated with barium (heparinized tubes) and the plasma samples with tin (collection tubes). The concentrations for Bi, Hg, Pb, Rb, Sb, and Sr in whole blood were within the literature ranges for healthy adults. All of the concentrations for Co, and some of the concentrations for Cd, Cs, Tl, and Zn were higher than the high limits of the normal ranges. Approximately 14% of the Cu concentrations were lower than the low limit of the normal range. The Mo and Sn concentrations are difficult to evaluate, because the normal ranges appears to be unreliable. All concentrations for Cd, Co, Mo, Pb, Sn, and Sr and some of the concentrations for Cu (15%) and Mn (75%) in the plasma samples were higher than the high limits of the normal ranges. The concentrations for Rb tended to be lower than the normal range. To establish unequivocally the causes for elevated and reduced concentrations of trace elements in whole blood and plasma of dialysis patients, all fluids in the dialysis process must be investigated.
Subject(s)
Kidney Failure, Chronic/blood , Renal Replacement Therapy , Trace Elements/blood , Adult , Humans , Kidney Failure, Chronic/therapy , Mass Spectrometry/methods , Quality Control , Reproducibility of Results , Spectrophotometry, AtomicABSTRACT
This paper presents views on the current status of (inorganic) arsenic risk assessment in the United States and recommends research needed to set standards for drinking water. The opinions are those of the Arsenic Task Force of the Society for Environmental Geochemistry and Health, which has met periodically since 1991 to study issues related to arsenic risk assessment and has held workshops and international conferences on arsenic. The topic of this paper is made timely by current scientific interest in exposure to and adverse health effects of arsenic in the United States and passage of the Safe Drinking Water Act Amendment of 1996, which has provisions for a research program on arsenic and a schedule mandating the EPA to revise the maximum contaminant level of arsenic in drinking water by the year 2001. Our central premise and recommendations are straightforward: the risk of adverse health effects associated with arsenic in drinking water is unknown for low arsenic concentrations found in the United States, such as at the current interim maximum contaminant level of 50 microg/l and below. Arsenic-related research should be directed at answering that question. New epidemiological studies are needed to provide data for reliable dose-response assessments of arsenic and for skin cancer, bladder cancer, or other endpoints to be used by the EPA for regulation. Further toxicological research, along with the observational data from epidemiology, is needed to determine if the dose-response relationship at low levels is more consistent with the current assumption of low-dose linearity or the existence of a practical threshold. Other recommendations include adding foodborne arsenic to the calculation of total arsenic intake, calculation of total arsenic intake, and encouraging cooperative research within the United States and between the United States and affected countries.
Subject(s)
Arsenic/adverse effects , Fresh Water/analysis , Water Pollutants, Chemical/adverse effects , Government Agencies , Humans , Neoplasms/chemically induced , Research , Risk Assessment , United StatesSubject(s)
Arsenic/urine , Arsenicals/urine , Environmental Exposure/analysis , Adolescent , Adult , Aged , Case-Control Studies , Child , Child, Preschool , England/epidemiology , Humans , Male , Middle Aged , Pilot ProjectsABSTRACT
Two microwave digestion systems (open-focused and closed-pressurized) were tested for the mineralization of human brain and bovine liver (NIST SRM 1577a) as dissolution steps prior to the determination of 16 trace elements (Bi, Cd, Co, Cs, Cu, Fe, Hg, Mn, Mo, Pb, Rb, Sb, Sn, Sr, Tl, and Zn) by inductively coupled plasma mass spectrometry (ICP-MS). Digestion parameters (mass of sample, digestion mixture, and power/time steps) were optimized using temperature and pressure sensors. Digestions with the open-focused microwave system require larger volumes of conc. HNO(3) and 30% H(2)O(2) than digestions with the closed-pressurized system. Both systems produce correct results for the bovine liver samples. The concentrations obtained for the digests of the open-focused system tend to be less precise than the concentrations from the "closed-pressurized" digests. Because the "open-focused" digests must be diluted to 50 mL to bring the acid concentration to 0.7-2.0 mol/L required by the ICP-MS (closed-pressurized digests need to be diluted to only 20 mL), the detection limits for the system with the open-focused digestion are higher than for the system with the closed-pressurized digestor. The open-focused digestor cannot handle more than 150 mg brain tissue, whereas the closed-pressurized system can mineralize 470 mg. The latter method gave better results with brain tissue than the open-focused system. The preparation of brain tissue as reference material for the determination of trace elements in brain samples is described.