ABSTRACT
The relative virulence of Cryptococcus neoformans serotypes A, B, C and D in four mouse strains was assessed by measuring their migration from the foot-pad of the animals to the spleen, lungs and brain in 6-week-old DBA/2, BALB/c, A/J and a hybrid mouse strain by re-isolating yeasts from the internal organs. Comparable doses of each C. neoformans serotype were inoculated into the foot-pads of the mice. C. neoformans var neoformans strains A68, D52, A-(IN) and D-(IN) were more virulent than C. neoformans var gatti strains B112 and C18. However, the differences in the relative virulence of the var neoformans and the var gatti serotypes for the mouse strains were not significant (p > 0.05). Re-isolation of yeasts from mice showed that the BALB/c mice, in particular, and the DBA/2 mice were more susceptible to disseminated C. neoformans infection. The virulence of C. neoformans serotypes through foot-pad inoculation of mice was established.
Subject(s)
Cryptococcosis/microbiology , Cryptococcus neoformans/pathogenicity , Animals , Brain/microbiology , Cryptococcus neoformans/classification , Cryptococcus neoformans/isolation & purification , Foot/microbiology , Lung/microbiology , Mice , Mice, Inbred A , Mice, Inbred BALB C , Mice, Inbred DBA , Serotyping , Spleen/microbiology , VirulenceABSTRACT
A serotype differentiation of the encapsulated yeast Cryptococcus neoformans is described using creatinine dextrose bromothymol blue thymine (CDBT) medium. On CDBT medium C. neoformans serotype D grew as bright red colonies, turning the medium a bright orange after five days incubation at 28 degrees C. C. neoformans serotype A grew as pale colonies with no apparent colour effect on the medium. Serotypes B and C caused a slight greening of the medium. The reaction of the four serotypes of C. neoformans on CDBT medium is considered useful in the differentiation of the closely related serotype A and D.
Subject(s)
Bromthymol Blue , Cryptococcus neoformans/classification , Serotyping/methods , Creatinine , Culture Media , Glucose , Hydrogen-Ion Concentration , ThymineABSTRACT
Enterotoxaemia caused by Clostridium perfringens type A in five intensively managed pigs is reported. The condition was precipitated by constipative digestive disturbance. Diarrhoea was not observed in the five animals before death.
Subject(s)
Clostridium perfringens/isolation & purification , Constipation/veterinary , Enterotoxemia/microbiology , Swine Diseases/microbiology , Animals , Constipation/microbiology , SwineABSTRACT
The agar-gel precipitation test (AGPT) was compared with Enzyme Immunoassay (EIA) for the detection of Chlamydia trachomatis, 164 women being screened for chlamydial antibodies and antigens in their sera and in endocervical specimens, respectively. The AGPT showed good correlation with EIA in the 164 paired sera and endocervical specimens, resulting in 27 (16.5%) and 22 (13.4%) positive results, respectively (P greater than 0.05). The overall sensitivity of the AGPT compared to EIA was 86.4% (19 of 22), and the specificity was 94.4% (134 of 142).
Subject(s)
Cervix Uteri/microbiology , Chlamydia Infections/diagnosis , Chlamydia trachomatis/isolation & purification , Adolescent , Adult , Female , Humans , Immunoenzyme Techniques , Middle Aged , Precipitin Tests , Sensitivity and SpecificitySubject(s)
Abortion, Veterinary/microbiology , Actinomyces/isolation & purification , Actinomycosis/veterinary , Cattle Diseases/microbiology , Abscess/microbiology , Abscess/veterinary , Actinomycosis/microbiology , Animals , Cattle , Female , Jaw Diseases/microbiology , Jaw Diseases/veterinary , PregnancyABSTRACT
A total of 690 adult cockroaches (Periplaneta americana (L.), the American cockroach, and Blatta orientalis (L.), the oriental cockroach) were captured alive within domestic kitchens and near poultry houses in Vom. Using selective media, their external surfaces and internal (gut) contents, after adequate decontamination of the external surfaces, were culturally examined for the presence of campylobacters. 4 isolates of Campylobacter subsp jejuni were made (0.5%); 3 from the gut contents and 1 from the external surface. Nocardia asteroides was isolated from the gut contents of a batch of ten cockroaches. The low isolation rate notwithstanding, our results suggest that cockroaches may be a potential vector of campylobacters from other sources to human food. The somewhat fortuitous isolation of Nocardia asteroides and its significance are discussed.
