[Abortive influenza virus infection of Ehrlich cells. Virion and subcellular fraction proteins]. / Abortivnaia infektsiia virusa grippa v kletkakh Erlikha. Belki virionov i subkletochnykh fraktsii.

Ehrlich ascitic carcinoma cells infected with the WSN strain of influenza virus produced noninfectious virus particles containing no M protein. Polypeptides P1, NP, and NS were synthesized in the infected ascites cells to the same concentration was in permissive cells (chick fibroblasts and MDCK cells); the synthesis of HA and M proteins was markedly disturbed, however. In the permissive cells, M protein was found in the fraction of the perinuclear martial and plasma membranes whereas in the ascitic cells M protein was detected only in perinuclear material but not in plasma membrane. Thus, nonpermissiveness of the ascitic cells appears to be due to disturbance of both synthesis and transport of M protein to the plasma membrane.

[Abortive influenza virus infection in Ehrlich ascitic carcinoma cells. Properties of virus particles and intracellular ribonucleoproteins]. / Abortivnaia infektsiia virusa grippa v kletkakh astsitnogo raka Erlikha. Svoistva virusnykh chastits i vnutrikletochnykh ribonukleoproteidov.

Ehrlich ascitic carcinoma cells infected with influenza virus, WSN strain, produced noninfectious virus particles differing by some properties from standard virions formed in chick embryo. The ascitic particles were unusually fragile and lost hemagglutinins during purification and storage. They were found in larger or smaller aggregates. In the electron microscope they appeared as spikeless particles of heterogeneous size. Polyacrylamide gel electrophoretic analysis of polypeptides revealed markedly decreased contents of hemagglutinins and M-protein. It is suggested that the decreased amount of M-protein results in the fragility of ascitic virus particles and loss of hemagglutinins which, in its turn, leads to the loss of infectivity. Ribonucleoproteins (RNP) synthesized in the cytoplasm and nuclei of Ehrlich ascitic carcinoma cells differed in their biophysical properties from RNP formed in the permissive system (chick fibroblast culture). They sedimented slower than RNP from chick fibroblasts, and their buoyant density was 1.37--1.40 g/ml instead of 1.34 g/ml, i. e. the density typical of influenza virus nucleocapsid.