ABSTRACT
A relatively simple, rapid sample preparation method has been developed for analysis of low-molecular mass compounds present in soluble coal products generated by microbial coal solubilizing agents. Acidification of the sample followed by direct extraction into hexanes is coupled with gas chromatography/mass spectrometry analysis for characterization of the soluble coal products. Characterization of the products can contribute to a more complete understanding of the solubilization processes involved, provide further information as to the structure of coal and identify products of potential commercial value.
Subject(s)
Coal/analysis , Arthrobacter/metabolism , Aspergillus/metabolism , Basidiomycota/metabolism , Biodegradation, Environmental , Gas Chromatography-Mass Spectrometry , Molecular Weight , Polyporaceae/metabolism , SolubilityABSTRACT
The ARCAT (A Rapid Coliphage Analysis Technique) method for detecting coliphages in water has been modified. Modifications to the original method include media optimization, the use of frozen host cultures, the use of a single agar coliphage assay and optimized plaque resolution with 2, 3, 4-triphenyltetrazolium chloride. Detection of 5 coliphages per 100 ml of water sample is accomplished in 6 hours for rapid estimation of water quality.
