ABSTRACT
Physical and mathematical considerations are presented in support of the suggestion that social hornets and bees, which construct brood combs with large arrays of cells in a honeycomb structure, exploit ultrasonic acoustic resonances in those cells in order to achieve the great accuracy of the hexagonal symmetry exhibited by these honeycomb-structured arrays. We present a numerical calculation of those resonances for the case of a perfect-hexagon duct utilizing a Bloch-Floquet-type theorem. We calculate the rate of energy dissipation in those resonances and use that, along with other considerations, to identify the resonance that is best suited for the suggested use by bees and hornets. Previously recorded ultrasonic data on social hornets and honeybees are cited which agree with some of our predictions and thus provide support for the above-mentioned suggestion.
Subject(s)
Bees/physiology , Behavior, Animal/physiology , Echolocation/physiology , Models, Biological , Nesting Behavior/physiology , Sonication , Wasps/physiology , Animal Communication , Animals , Computer Simulation , Social BehaviorABSTRACT
The Oriental hornet Vespa orientalis (Hymenoptera, Vespinae) coordinates its daily activities (e.g. flights out of the nest associated with digging activities and removal of the dug soil from the nest) with the amount of insolation. Thus, the stronger the insolation, the more intense the flight activity and vise versa. The hornet's cuticle bears a few yellow stripes interposed among brown parts of the gastral cuticle. These yellow stripes are composed of two elements, namely, a transparent cuticle and underneath it a layer of yellow granules. When the hornets are exposed to UV light, the layer containing the yellow granules is less active than in hornets kept in the dark. This diminished activity entails a lower production of glucose as well as of several enzymes prevalent also in the liver of mammals, like creatine kinase, alanine aminotransferase, aspartate transaminase. Thus solar irradiation stimulates and produces a change in the metabolic activities of the hornet. The fact that hornets link their flight activity with the insolation leads us to speculate that the sun contributes energetically to the hornet's activity.
Subject(s)
Ultraviolet Rays , Wasps/anatomy & histology , Wasps/physiology , Animals , Liver/physiology , Liver/radiation effects , Liver Function Tests , Pigments, Biological , Wasps/radiation effectsABSTRACT
The Oriental hornet bears both brown and yellow colors on its cuticle. The brown component is contributed by the pigment melanin, which is dispersed in the brown cuticle and provides protection against insolation, while the yellow-colored part contains within pockets in the cuticle granules possessing a yellow pigment. These yellow granules (YG) are formed about 2 days prior to eclosion of the imago, and their production continues for about 3 days posteclosion. Xanthopterin is the main component of the granule and lends it its yellow color. Xanthopterin produces a characteristic excitation/emission maximum at 386/456 nm. Characterization by use of mass spectrometry showed the compound to have a molecular ion of 179, as expected from xanthopterin. Spectroscopic examination of the absorption of an entire stripe of yellow cuticle in the course of its metamorphosis revealed that the absorption steadily increases throughout the process to a maximal level of absorption about 3 days posteclosion. In the absence of the YG, the cuticle is permeable to the passage of all wavelengths within the visible range and to the UV range (290-750 nm) in all age groups of hornets. The newly ecloded hornets depart the nest to engage in activities requiring exposure to insolation only as the process of granule formation terminates, namely, when the layer of YG in the cuticle suffices to absorb all the harmful UV radiation.
Subject(s)
Bees/metabolism , Light , Pigments, Biological , Xanthopterin/metabolism , Animals , Bees/growth & developmentABSTRACT
During the active season, extending from June to October, hornets emerge from their nest in the field in all the daytime hours. In the beginning of the season, when the number of workers is relatively small, the number of exits from the nest is fairly uniform numerically throughout the day. However, with the increase in hornet population from July onwards, the number of workers emerging from the nest entrance around noon (1100-1300 h) is by 1-2 orders of magnitude greater than the number of those emerging in the morning or evening hours. This disparity persists till September or October, at which time the workers revert to behave as in the beginning of the season. It appears, therefore, that in this period hornet activities outside the nest are coordinated with the meteorological conditions, and in this regard, the highest correlation is with the ultra violet B (UVB) radiation level and to a lesser extent with the temperature. Presumably, also, the greater noon-hour activity in the nests of hornets in the field stems from the digging hornets benefiting from the greater availability of solar energy at noon, mainly that of UVB radiation. We assume that the hornets are able to utilize the UVB radiation, but what part of their body is "absorbing" the UVB energy is still a matter of further investigation.
Subject(s)
Behavior, Animal/radiation effects , Humidity , Temperature , Wasps/radiation effects , Animals , SeasonsABSTRACT
Social wasps and hornets maintain their nest in the dark. The building of the combs by all Vespinae is always in the direction of the gravitational force of Earth, and in each cell's ceiling, at least one 'keystone' is embedded and fastened by saliva. The sensory mechanisms that enable both building of sizeable symmetrical combs and nursing of the brood in the darkness merit investigation, and the aim of the present study was to identify and characterize the 'keystones' that exist in the ceiling and in the walls of the social wasp comb cells. Bio-ferrography was used to isolate magnetic particles on slides. These slides, as well as original cells, were analyzed in an environmental scanning electron microscope by a variety of analytical tools. It was found that both the roof and the walls of each comb cell bear minerals, like ferrites, as well as Ti and Zr. The latter two elements are less abundant in the soil around the nest. Ti and Zr are known to reflect infrared (IR) light. IR imaging showed a thermoregulatory center in the dorsal thorax of the adult Oriental hornet. It is not known yet whether these insects can sense IR light.
Subject(s)
Nesting Behavior , Orientation , Wasps/physiology , Animals , Gravitation , Minerals/analysis , Social BehaviorABSTRACT
In the Oriental hornet, a thermogenic center is located in its prothorax. The present study attempted to elucidate the development of this organ with age, that is, by following the development of the thermogenic center in the hornet from its pupal stage until several days after eclosion of the imago. To this end, use was made of an infrared camera, with which pictures were taken of the prothorax in hornets at various ages, i.e., several days prior eclosion, 24 h after eclosion, and 48-h posteclosion. The photographic findings established that prior to 48-h posteclosion there was no thermally distinct region or spot in the prothorax, but at about 48 h, such a "hot spot," namely, a point whose temperature is greater than that of the rest of the prothorax, does appear, and its appearance coincides with certain specific nest activities like warming of the pupae. Next, an attempt was made to transplant by allograft the region in the prothorax housing the hot spot. Accordingly, several pupae at 2 days prior to eclosion were subjected to the following procedure; their future prothoracic thermogenic center was excised and so also an equally sized piece of cuticle from the dorsal region of their abdomen, and the two now allografted in exchange, i.e., the piece from the prothorax replacing the abdominal piece and vice versa. The result of this exchange-transplant was studied 48 h after eclosion of the operated hornets and showed disruption of heat formation in the prothoracic site coupled with nonappearance of a hot spot in the abdominal site. As for the functional, intact hot spot in the adult hornet, it is characterized by a high concentration of tracheae, with numerous mitochondria in between them that probably contribute to the heat generation.
Subject(s)
Wasps/physiology , Wasps/ultrastructure , Animals , Body Temperature , Infrared Rays , Larva , Microscopy, Electron, Scanning , Pupa , Thermography , Wasps/anatomy & histologyABSTRACT
We propose that social hornets and bees, who construct large arrays (known as combs) of cells for hatching and brooding their offspring, exploit ultrasonic acoustic resonances in those cells in order to implement the accurate honeycomb structure exhibited by those arrays. This idea is supported by a number of theoretical considerations, including a detailed analysis of the spectrum of lateral acoustic resonances in a cell with circular cross section, considered as an approximation to the actual perfect hexagon shape. It is also supported by the results of some previous measurements of the acoustic spectrum in a nest of Oriental hornets.
Subject(s)
Acoustics , Models, Biological , Nesting Behavior/physiology , Algorithms , Animal Communication , Animals , Bees , Ultrasonics , WaspsABSTRACT
Oriental hornet workers, kept in an Artificial Breeding Box (ABB) without a queen, construct within a few days brood combs of hexagonal cells with apertures facing down. These combs possess stems that fasten the former to the roof of the ABB. In an ABB with adult workers (more than 24 h after eclosion), exposed to an AC (50 Hz) magnetic field of a magnitude of B = 50-70 mGauss, the combs and cells are built differently from those of a control ABB, subjected only to the natural terrestrial magnetic field. The effects of the additional magnetic field consist of (a) 35-55% smaller number of cells and fewer eggs in each comb, (b) disrupted symmetry of building, with many deformed and imperfectly hexagonal cells, and (c) more delicate and slender comb stems.
Subject(s)
Magnetics , Nesting Behavior/physiology , Wasps/physiology , Animals , MaleABSTRACT
In social hornets of sub-family Vespinae there are stripes or areas in the cuticle which are endowed with a bright color (yellow, orange, green) that is different from the customary native brown color. In these brightly colored areas, the middle and bottom layers of the cuticle contain a yellow-colored filling material instead of the ordinary lamellae of which the cuticle is comprised. At high magnification, most of the yellow matter is seen to be made up of yellow granules interlinked by string-like threads. Apart from yellow granules, hemolymph and a surrounding cuticle, these areas are also found to contain glucose, iron and triglycerides as well as enzymes like GOT, GPT, CPK and LDH, in high concentrations that vary considerably from those in vespan hemolymph and whose levels are different under illumination than in the dark. Such high levels of enzymes suggest first an association with liver function activities, and secondly, that the difference in their levels when exposed to light and when subjected to darkness is indicative of the influence of the energy of light upon them.
Subject(s)
Wasps/anatomy & histology , Wasps/metabolism , Abdomen/anatomy & histology , Abdomen/physiology , Alanine Transaminase/metabolism , Animals , Aspartate Aminotransferases/metabolism , Glucose/metabolism , Hemolymph/metabolism , Iron/metabolism , Liver/enzymology , Microscopy, Electron, Scanning , Pigmentation , Triglycerides/metabolism , Wasps/ultrastructureABSTRACT
Wasps apparently develop normally even under extreme thermal conditions, including deserts. We deemed it worthwhile to set up an experiment wherein wasp brood combs containing a full gamut of brood ranging from eggs up to pupae and a few adults were kept in an incubator whose temperature was gradually raised to 45 degrees C, and the response of the disparate brood to such warming was photographed via Infra Red camera. The finding of this experiment showed that for open brood (i.e., eggs, larvae at various instars, and empty cells) the temperature was close to the ambient temperature, but in the silk coated pupae, the temperature was lower than the ambient by up to 4 degrees C. This lower temperature was retained for at least 90 min of incubation. For comparison we evaluated the relative contribution of the pupae to the phenomenon, by warming also a vacant, (i.e., a broodless and silkless comb) in parallel to a comb from which the pupae had been extricated but the silk weave retained and left behind. We found that the totally empty comb heated up under these conditions to nearly 110 degrees C, whereas the silk-containing vacant cells only heated up to about 40 degrees C. These finding are discussed from two aspects, namely the importance for wasps to maintain a constant temperature throughout the pupating process, and the manner in which the silk weave contributes to such a goal.
Subject(s)
Body Temperature Regulation/drug effects , Fever/prevention & control , Silk/pharmacology , Wasps/growth & development , Wasps/physiology , Animals , Larva/growth & development , Nesting Behavior , Pupa/growth & development , Silk/metabolism , TemperatureABSTRACT
In a previous study, it was shown that the hornet venom or, more specifically, its venom sac extract (VSE) possesses deoxyribonuclease activity that exerts an effect both on insects as well as on mammals. We have now examined the effect of hornet VSE on primary culture of rat cortical neurons. Judging on the basis of our results, VSE induces a rapid cell death by a) permeabilizing the cell membrane, b) inducing DNA breaks, and c) cleaving the nuclear protein poly-ADP-ribose polymerase (PARP-1), thereby preventing DNA repair.
Subject(s)
Cerebral Cortex/cytology , Neurons/drug effects , Neurons/metabolism , Wasp Venoms/toxicity , Animals , Cell Death/drug effects , Cells, Cultured , DNA Breaks , Female , Poly (ADP-Ribose) Polymerase-1 , Poly(ADP-ribose) Polymerases/metabolism , Rats , Rats, Sprague-DawleyABSTRACT
In both the large carpenter bee (Xylocopa pubescens) and the bumblebee (Bombus terrestris), a hot spot was detected in the center of the prothorax on its dorsal-external aspect. In both cases, the temperature in this hot spot was found to be greater than the ambient temperature and that at the tip of the gaster. In B. terrestris, it was higher by 9-10 degrees C from that at the gaster tip and by 15-16 degrees C from the ambient temperature, while in X. pubescens the corresponding differences were 11-20 degrees C and 18-19 degrees C, respectively. The recorded thermal differences were not fixed but were rather variable, temporally as well as individually, but invariably all individuals measured showed these temperature differences. Furthermore, in none of the studied specimens was a hot spot detected in any part of the body other than the prothorax. From this hot spot in the prothorax, there is a cascade of temperatures in both directions, that is, anteriorly towards the head and posteriorly towards the gaster, with a graded drop in temperature in either direction. This article discusses possible reasons for the existence of such a hot spot in this particular location (the prothorax), its role or function, and its mode of operation. The authors speculate that it is a thermoregulatory center (for heating or cooling) that might be present in possibly all Hymenoptera that spend a considerable part of their life flying, regardless of whether they are social, parasocial, or solitary.
Subject(s)
Bees/anatomy & histology , Bees/physiology , Animal Structures/physiology , Animal Structures/ultrastructure , Animals , Body Temperature , Microscopy, Electron, Scanning , ThermographyABSTRACT
In the social wasps Vespa orientalis and Paravespula germanica (Hymenoptera, Vespinae), a thermogenic center has been found in the dorsal part of the first thoracic segment. The temperature in this region of the prothorax is higher by 6-9 degrees C than that at the tip of the abdomen, and this in actively flying hornets outside the nest (workers, males or queens) as well as in hornets inside the nest that attend to the brood in the combs. On viewing the region from the outside, one discerns a canal or rather a fissure in the cuticle, which commences at the center of the dorsal surface of the prothorax and extends till the mesothorax. Thus the length of this canal or fissure is approximately 5-7 mm and it is seen to contain numerous thin hairs whose shape varies from that of the hairs alongside the structure. Beneath the cuticle in this region there are dorsoventral as well as longitudinal muscles in abundance, much the same as the musculature in the remaining thoracic segments (i.e. the meso- and metathorax), which activate the two pairs of wings. The canal-bearing segment is of course devoid of wings, and its dorsoventral muscles are attached to the cuticle, which in this region resembles a bowl harboring several layers of epithelium that boasts numerous butterfly-shaped tracheal branches. Additionally there are layers that display lymph-filled spaces and also perforated layers and depressions, and beneath all these is a lace-like layer that also coats the cuticle's hollows. Underneath the cuticle proper, there are numerous large mitochondria and tracheae, which occupy a considerable part of the cuticular epithelium surface. These abundant mitochondria are, most probably, the main element of heat production in the thermogenic center.
Subject(s)
Body Temperature Regulation/physiology , Thorax/anatomy & histology , Wasps/anatomy & histology , Animals , Infrared Rays , Male , Microscopy, Electron, Scanning , Photography , Thorax/physiology , Wasps/physiologyABSTRACT
This paper deals with hairs and organelles present on the head and antennae of the German wasp, Paravespula germanica, and their possible role in sensing the physical and chemical ambience, as well as in intercommunicating both while in flight outside or in the nest. Via scanning electron microscope photography, we detected on the frons plate of the wasp's head, hairs that were about 300 microm long and comprised the longest hairs on the body of the wasps. Additionally, the two antennae bore along their entire length photoreceptors, placoids, campaniforms, trichoids, and agmons. These organelles are located at high but variable density along the antennal segments. The paper provides the dimensions of each of the mentioned organelles, and discusses the possible functions of the organelles as well as of the hairs on the frons. Photographs taken via atomic force microscope reveal that the epicuticle of the antenna is of two typical shapes; one, bearing both longitudinal stripes as well as transverse bands that are about 1 mum in width, and a second granulated form. Conceivably, the wasp uses the various organelles mentioned to communicate with its mates that are some distance away, somewhat like the use of radar by humans.
Subject(s)
Wasps/ultrastructure , Animals , Extremities , Hair/ultrastructure , Head , Microscopy, Atomic Force , Microscopy, Electron, Scanning , Photoreceptor Cells, Invertebrate/ultrastructure , Sense Organs/ultrastructureABSTRACT
Feeding of diazepam to young hornets completely inhibits or delays development of their ovaries for a relatively long period. In control hornets, the ovaries usually develop within a day or two post eclosion and comb building commences on the second day of life. The hornets then oviposit into the comb cells and the deposited ova give rise to larvae. Trials were performed on parallel groups of hornets of various ages. When the sedative diazepam was administered to hornets aged 0-24 hours the ovaries of these young hornets failed to show any development, so that no oocytes ripened and consequently there was no oviposition whatsoever. Neither were any comb cells built or, at best, only a few were built. When the diazepam was administered to hornet's being the age of 48 hours, it exerted no change, that is, the eggs developed normally and comb building was the same as in the control group. Longevity of hornets was uniform in all the test groups and similar to that in the control.
Subject(s)
Diazepam/administration & dosage , Motor Activity/physiology , Ovary/growth & development , Oviposition/physiology , Receptors, GABA-A/metabolism , Sexual Behavior, Animal/physiology , Wasps/drug effects , Wasps/physiology , Administration, Oral , Animals , Female , Hypnotics and Sedatives/administration & dosage , Male , Motor Activity/drug effects , Ovary/drug effects , Oviposition/drug effects , Reproduction/drug effects , Reproduction/physiology , Sexual Behavior, Animal/drug effects , Social BehaviorABSTRACT
The head of the Oriental hornet in situ, detached from a live sample was imaged using Magnetic Resonance Imaging (MRI). This non-invasive method enabled us to visualize the three-dimensional structure of the hornet's brain and intracerebral organs, as based on cubic voxels of 23 microm3. From these images, we could identify various cephalic structures in both supra-esophageal and sub-esophageal locations. In the former location, we identified and visualized the ocelli, ommatidia, mushroom body, lobula, medulla and the compound eyes in the protocerebrum, as well as the olfactory lobe and bases of the antenna in the deutocerebrum, while in the sub-esophageal region we visualized organs such as the mouthparts, the esophagus, the gnathal pouch and the salivary ducts that empty into the region. Additionally, we identified various muscles, the aorta, cuticular thickenings lending support to the interior of the head and also the cuticular skeleton providing support on the outside. All the mentioned structures and organs were visualized in their relative, normal proportions, without touching or dislocating them.
Subject(s)
Brain/anatomy & histology , Head/anatomy & histology , Magnetic Resonance Imaging , Wasps/anatomy & histology , Animals , FemaleABSTRACT
In the Oriental hornet Vespa orientalis (Hymenoptera, Vespinae), there is on the dorsal side of the thorax, beneath the mesoscutum plate of the prothorax and around the median notal suture, a lump that, in the course of hornet activity, is warmer by 9 degrees C from the surrounding milieu and by up to 6 degrees C from other body parts of the hornet. This lump is about 1 mm in diameter, butterfly-shaped, and its upper, posterior border abuts the base of the forewings. During hornet activity and via Infra Red photography one can observe heat extensions stemming from the center of the lump and proceeding forward in the direction of the head, downward toward the legs and backwards toward the bases of the wings. The warmest region is the center of the lump, with its margins showing a lower temperature. As for the legs of the hornet, their upper part is warmer than the other parts. The temperature gradients along the hornet's body are dependent on the extent and nature of hornet activity. Thus, during flight or ventilation activity, the thorax is the warmest part of the body, while the wings, legs, and antennae, as well as the posterior part of the gaster are colder, yet all these body parts are still warmer to varying degrees than the surrounding milieu. Thus, at night, when sentry worker hornets stand guard around the nest entrance and remain practically motionless, the temperature differences between the various body parts are retained unchanged. We conjecture that the described butterfly-shaped lump is a thermoregulatory center (TC), which is neurogenically activated, since the changes occurring in it are rapid, a matter of one to several seconds and do not appear to be directly dependent on the hemolymph supply. The thermoregulatory center keeps a high constant temperature apparently related to hornet activity and the environmental conditions. The temperature cascade is most probably regulated via the tracheal system. Apparently another system activated by a heat pump mechanism keeps the gaster at a lower temperature than the environment.
Subject(s)
Body Temperature/physiology , Wasps/physiology , Animals , Body Temperature Regulation , Infrared Rays , Photography , TemperatureABSTRACT
In the head of the Oriental hornet, beneath the cuticle, there are plaques of hair cells. These are distributed throughout the upper front part of the head; to wit: in the region of the vertex (i.e., around and behind the ocelli), in the genae around and behind the compound eyes (the ommatidia), and in the region of the forehead or frons. These hair cells are arranged with their thin whip-like part (i.e., cilia) directed outward and morphologically fall into three distinct groups: type (a) thin elongated cilia connected to each other alongside by side-links; type (b) thin elongated cilia of which two or more interconnect at their distal ends via a delicate nerve fiber bearing a knob at its center; and type (c) shorter and thicker cilia that roughly resemble a triangular thorn and are also interconnected by a thin thread, which, however, bears a ball rather then a knob at its center. The knob in the one case and the ball in the other vary in their diameter, but in both instances the interconnecting elements, be they nerve fibers or threads, are seemingly multidirectional. Beneath the frons, in the region of the coronal suture, the hair cells (cilial plaques) are inwardly directed and bear a large trachea at their center. Presumably, the "weighted" cilial cells that are directed toward the exterior of the body aid the hornet in navigation and gravity determination whereas the inwardly directed ciliary cells may possibly serve in acoustic communication. Another element worthy of mention within the hair cells are yellow granules (yg). These yg's originate from the whip-like portion of the ciliary cells that are distributed beneath the frons plate, and also in the yellow stripes of the gastral cuticle. Conceivably, these yellow granules, in both cases, may play a role in the absorption and storage of solar energy. In summary, ciliary structures are involved in the hornet in gravity sensing, in acoustical communication and in light sensing, i.e., with some similarity with what happens in vertebrates in the inner ear and in the photoreceptor.
Subject(s)
Wasps/anatomy & histology , Animals , Cilia/ultrastructure , Cytoplasmic Granules/ultrastructure , Flagella/ultrastructure , Gravity Sensing , Hair Cells, Auditory/ultrastructure , Head/anatomy & histology , Head/physiology , Microscopy, Electron , Wasps/physiology , Wasps/ultrastructureABSTRACT
Hornet silk is a polymer of amino acids. One of the known properties of polymers is their electrical activity. The present study describes the results of electrical measurements carried out vertically on the silk cap of pupae of the Oriental hornet Vespa orientalis (Hymenoptera, Vespinae). The measurements undertaken were the temperature-dependent electric current, voltage and resistance, all measured within the range of biological temperatures, as well as the capacitance. The temperature-dependent spontaneous current attained values up to 327 nano Amperes (nA) while the maximal voltage reached 347 millivolt (mV). The electrical resistance was low and steady (1-20 mu omega) at temperatures ranging between 19-32 degrees C, but at lower or higher temperature it increased fairly sharply by about three orders of magnitude. The electrical capacitance, computed according to the discharge curve (decay curve) amounted to 0.4 microFarad (microF). The paper also discusses the role of the pupal silk as producer of a 'clean room' while the cuticle is being laid down by the pupae after undergoing metamorphosis, as well as the significance of the measured electrical parameters vis-à-vis the developing pupae.
Subject(s)
Electric Conductivity , Electrophysiology , Pupa/physiology , Silk/chemistry , Wasps/chemistry , Animals , Insect Proteins/chemistry , Insect Proteins/metabolism , Metamorphosis, Biological , Models, Biological , Silk/metabolism , Spectrum Analysis, Raman , Temperature , Time Factors , Wasp Venoms/toxicityABSTRACT
The Oriental hornet Vespa orientalis (Hymenoptera, Vespinae) flies outside its nest only in the daytime and never in the dark. Oriental hornets can be anaesthetized by means of diethyl ether, following which they awake spontaneously within about > or =1 h. However, when the anaesthetized hornets are exposed to direct irradiation they awake much faster and immediately fly out and this is within the temperature range for their normal activities (20-40 degrees C). Light exerts an effect not only on intact hornets, but also on their main body parts, be they the head, thorax or gaster, or a torso without a head or without a gaster. These body parts also 'awake' from ether anaesthesia when illuminated, especially by ultraviolet (UV) B or shorter light wavelengths, but a body without a gaster awakes much later than normal. As for flight activity, headless hornets do not fly at all, while hornets devoid of a gaster flutter their wings when illuminated but are incapable of rising in the air. When vespan cuticular regions are coated with paint that blocks the passage of light, such as UVB blockers, or with white Tipp-Ex, the awakening from anaesthesia is delayed. The vespan cuticle evinces photovoltaic activity, even in dead specimens, so that upon exposure to light it is possible to record levels of 30-180 mV, both in a wakeful hornet and in one that has undergone anaesthesia. We conjecture that in the awake hornet the voltage that its cuticle generates under the influence of light is transported within the cuticular interlamellar membranes to be utilized as flight energy by the muscles attached to them; however, in the narcotized hornet, the diethyl ether apparently decreases or disrupts membranal order, blocking any ionic channel activity and thereby inducing the anaesthesia. Presumably, this state of phase transition is remedied spontaneously, albeit in a lengthy process, or else the light energy in UV speeds up restoration of the ionic channel activity and the synaptic transmission and thereby contributes to a more rapid awakening. In this regard, the hornet gaster is an extensive region for absorbing light, which explains why its absence considerably delays the awakening from anaesthesia.