ABSTRACT
The dynamics of the isolation of V. cholerae cultures from various water objects on the territory of Rostov-on-Don during the period of 1994-2001 was analyzed and biological properties of 14 such cultures were studied. In the absence of epidemic complications during the above-mentioned period, a growth in the amount of V. cholerae isolates, serogroups 01 and 0139, including toxigenic V. cholerae 01, was registered. The microbiological and epidemiological aspects of the monitoring of surface reservoirs and sewage were considered and the expediency of the profound and systematic study of its results for epidemiological surveillance on cholera was emphasized.
Subject(s)
Sewage/microbiology , Vibrio cholerae/isolation & purification , Cholera/diagnosis , Cholera/epidemiology , Humans , Population Surveillance , Retrospective Studies , Russia/epidemiology , Serotyping , Vibrio cholerae/classification , Vibrio cholerae/physiology , Water MicrobiologyABSTRACT
Below is given a procedure of the obtaining diagnostic fluorescent monoclonal immunoglobulin to detect cholera vibrios of O139 serovar. While obtaining preparations it was managed to determine optimal FTTS-MKA ratio, duration of their conjugation, series of fluorochrome. Test specimens of fluorescent monoclonal immunoglobulin provides intensive glow of V cholerae O139 cells in the working dilution 1:16-1:32. Tests of diagnostic FTTS-MKA on the great number of homologic and heterologic strains showed their strict specificity and high sensibility as to cholera vibrios of O139 serogroup.
Subject(s)
Antibodies, Monoclonal , Fluorescent Antibody Technique, Direct , Immunoglobulins , O Antigens/immunology , Vibrio cholerae/immunology , Antibodies, Monoclonal/immunology , Antibody Specificity , Humans , O Antigens/analysis , Sensitivity and Specificity , Serotyping , Vibrio cholerae/isolation & purification , Water MicrobiologyABSTRACT
The development of a method for serological identification of toxigenic and nontoxigenic V. cholerae non-O1, as well as on their role in human pathology, is reviewed. The evaluation on this method when used for establishing the etiology of acute diarrhea cases and analysis of sporadic diseases and group outbreaks of alimentary toxicoinfection type. Different points of view on the formation of toxigenic clones of V. cholerae non-O1 in nature is considered. The necessity for further development of the serological typing method is substantiated and the tasks of its improvement are set.