[Dynamics of the isolation and biological properties of Vibrio cholerae cultures, serogroups 01 and 0139, isolated from water reservoirs and sewage water on the territory of the city of Rostov-on-Don]. / Dinamika vydeleniia i biologicheskie svoistva kul'tur kholernykh vibrionov 01 i 0139 serogrupp, vydelennykh iz poverkhnostnykh vodoemov i stochnykh vod na territorii g. Rostov-na-Donu.

The dynamics of the isolation of V. cholerae cultures from various water objects on the territory of Rostov-on-Don during the period of 1994-2001 was analyzed and biological properties of 14 such cultures were studied. In the absence of epidemic complications during the above-mentioned period, a growth in the amount of V. cholerae isolates, serogroups 01 and 0139, including toxigenic V. cholerae 01, was registered. The microbiological and epidemiological aspects of the monitoring of surface reservoirs and sewage were considered and the expediency of the profound and systematic study of its results for epidemiological surveillance on cholera was emphasized.

[Obtaining diagnostic fluorescent monoclonal immunoglobulins against the V. cholerae 0139-serovar]. / Poluchenie diagnosticheskikh fliuorestsiruiushchikh monoklonal'nykh immuhoglobulinov k V. cholerae 0139-serovara.

Below is given a procedure of the obtaining diagnostic fluorescent monoclonal immunoglobulin to detect cholera vibrios of O139 serovar. While obtaining preparations it was managed to determine optimal FTTS-MKA ratio, duration of their conjugation, series of fluorochrome. Test specimens of fluorescent monoclonal immunoglobulin provides intensive glow of V cholerae O139 cells in the working dilution 1:16-1:32. Tests of diagnostic FTTS-MKA on the great number of homologic and heterologic strains showed their strict specificity and high sensibility as to cholera vibrios of O139 serogroup.

[Evaluation of a method for the serological identification of Vibrio cholerae non-01]. / Otsenka metoda serologicheskoi identifikatsii Vibrio cholerae ne 01.

The development of a method for serological identification of toxigenic and nontoxigenic V. cholerae non-O1, as well as on their role in human pathology, is reviewed. The evaluation on this method when used for establishing the etiology of acute diarrhea cases and analysis of sporadic diseases and group outbreaks of alimentary toxicoinfection type. Different points of view on the formation of toxigenic clones of V. cholerae non-O1 in nature is considered. The necessity for further development of the serological typing method is substantiated and the tasks of its improvement are set.