Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Dokl Biochem Biophys ; 516(1): 53-57, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38700816

ABSTRACT

Study of CD4+ T cell response and T cell receptor (TCR) specificity is crucial for understanding etiology of immune-mediated diseases and developing targeted therapies. However, solubility, accessibility, and stability of synthetic antigenic peptides used in T cell assays may be a critical point in such studies. Here we present a T cell activation reporter system using recombinant proteins containing antigenic epitopes fused with bacterial thioredoxin (trx-peptides) and obtained by bacterial expression. We report that co-incubation of CD4+ HA1.7 TCR+ reporter Jurkat 76 TRP cells with CD80+ HLA-DRB1*01:01+ HeLa cells or CD4+ Ob.1A12 TCR+ Jurkat 76 TRP with CD80+ HLA-DRB1*15:01+ HeLa cells resulted in activation of reporter Jurkat 76 TPR after addition of recombinant trx-peptide fusion proteins, containing TCR-specific epitopes. Trx-peptides were comparable with corresponding synthetic peptides in their capacity to activate Jurkat 76 TPR. These data demonstrate that thioredoxin as a carrier protein (trx) for antigenic peptides exhibits minimal interference with recognition of MHC-specific peptides by TCRs and consequent T cell activation. Our findings highlight potential feasibility of trx-peptides as a reagent for assessing the immunogenicity of antigenic fragments.


Subject(s)
CD4-Positive T-Lymphocytes , Peptides , Receptors, Antigen, T-Cell , Recombinant Fusion Proteins , Thioredoxins , Humans , Thioredoxins/immunology , Thioredoxins/genetics , Jurkat Cells , CD4-Positive T-Lymphocytes/immunology , Receptors, Antigen, T-Cell/immunology , Receptors, Antigen, T-Cell/metabolism , Recombinant Fusion Proteins/pharmacology , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/immunology , Peptides/pharmacology , Peptides/immunology , Peptides/chemistry , Lymphocyte Activation/drug effects , HeLa Cells
SELECTION OF CITATIONS
SEARCH DETAIL
...