Defense strategies and associated phytohormonal regulation in Brassica plants in response to chewing and sap-sucking insects.

Plants have evolved distinct defense strategies in response to a diverse range of chewing and sucking insect herbivory. While chewing insect herbivores, exemplified by caterpillars and beetles, cause visible tissue damage and induce jasmonic acid (JA)-mediated defense responses, sucking insects, such as aphids and whiteflies, delicately tap into the phloem sap and elicit salicylic acid (SA)-mediated defense responses. This review aims to highlight the specificity of defense strategies in Brassica plants and associated underlying molecular mechanisms when challenged by herbivorous insects from different feeding guilds (i.e., chewing and sucking insects). To establish such an understanding in Brassica plants, the typical defense responses were categorized into physical, chemical, and metabolic adjustments. Further, the impact of contrasting feeding patterns on Brassica is discussed in context to unique biochemical and molecular modus operandi that governs the resistance against chewing and sucking insect pests. Grasping these interactions is crucial to developing innovative and targeted pest management approaches to ensure ecosystem sustainability and Brassica productivity.

Correction: Exploring factors influencing patient mortality and loss to follow-up in two paediatric hospital wards in Zamfara, North-West Nigeria, 2016-2018.

[This corrects the article DOI: 10.1371/journal.pone.0262073.].

Enhancement of antibacterial and UV protection properties of blended wool/acrylic and silk fabrics by dyeing with the extract of Mimusops elengi leaves and metal salts.

In response to the heightened awareness of infectious diseases and the growing emphasis on personal protection in daily life, the utilization of natural bioresources for textile fabric dyeing has garnered substantial research attention. This is particularly due to their ability to confer antibacterial and UV protection properties to fabrics. In this study, the dyeing properties of Mimusops elengi Linn extract, alone and mordanted, were evaluated on blended wool/acrylic and silk fabrics, along with an assessment of their antibacterial and UV protection characteristics. The dyed fabrics exhibited good color strength and color fastness. Quantitative assessment of antibacterial activity was conducted using the reduction percentage test, while UV protection properties were determined through the measurement of Ultraviolet Protection Factor (UPF). Aqueous extract alone, when applied to blended wool/acrylic fabric, demonstrated an impressive 99.88 % reduction against Staphylococcus aureus, and 48.33 % for silk fabric, albeit less effective against Escherichia coli. Notably, when fabrics were dyed with a combination of leaves extract and various metal salt mordants, a substantial improvement in antibacterial properties was observed. Zinc and copper salts, in particular, exhibited the ability to enhance antibacterial properties to almost 100 % against Staphylococcus aureus and Escherichia coli in both blended wool/acrylic and silk fabrics. Concurrently, this combination contributed to an increase in the UV protection property of both fabrics. The findings underscore the potential of plant-based natural dye for blended wool/acrylic and silk fabrics, imparting antimicrobial and UV protection properties. This has significant implications in preventing the spread of infections and skin diseases, emphasizing the vital role of such textiles in promoting health and well-being.

Heat generation/absorption effect on natural convective heat transfer in a wavy triangular cavity filled with nanofluid.

This study is numerically executed to investigate the influence of heat generation or absorption on free convective flow and temperature transport within a wavy triangular enclosure filled by the nanofluid taking the Brownian effect of nanoparticles. The water (H2O) is employed as base fluid and copper (Cu) as nanoparticles for making effective Cu-H2O nanofluids. The perpendicular sinusoidally wavy wall is cooled at low temperature while the horizontal bottom sidewall is heated non-uniformly (sinusoidal). The inclined wall of the enclosure is insulated. The governing dimensionless non-linear PDEs are executed numerically with the help of the Galerkin weighted residual type finite element technique. The numerically simulated results are displayed through average Nusselt number, isothermal contours, and streamlines for the various model parameters such as Hartmann number, Rayleigh number, heat generation or absorption parameter, nanoparticles volume fraction, and undulation parameter. The outcomes illustrate that the temperature transport rate augments significantly for the enhancement of Rayleigh number as well as nanoparticles volume fraction whereas reduces for the increment of Hartman number. The heat transfer is significantly influenced by the size, shape, and Brownian motion of the nanoparticles. The rate of heat transport increases by 20.43% considering the Brownian effect for 1% nanoparticle volume. The thermal performance increases by 8.66% for the blade shape instead of the spherical shape of nanoparticles. In addition, heat transfer is impacted by the small size of nanoparticles. The thermal transport rate increases by 35.87% when the size of the nanoparticles reduces from 100 to 10 nm. Moreover, the rate of heat transmission increases efficiently as the undulation parameter rises. It is also seen that a crucial factor in the flow of nanofluids and heat transmission is the heat generation/absorption parameter that influences temperature distribution, heat transfer rates, and overall thermal performance.

Identifying regional disparities of infant mortality rates in Bangladesh: insights from nationwide cross-sectional studies and a statistical modelling approach using linear mixed effects model with temporal variability.

OBJECTIVE: The major objective of this project is to find the best suitable model for district-wise infant mortality rate (IMR) data of Bangladesh over the period 2014-2020 that captures the regional variability and overtime variability of the data. DESIGN, SETTING AND PARTICIPANTS: Data from seven consecutive cross-sectional surveys that were conducted in Bangladesh between 2014 and 2020 as a part of the Sample Vital Registration System (SVRS) were used in this study. The study included a total of 13 173 (with 390 infant deaths), 17 675 (with 512 infant deaths), 17 965 (with 501 infant deaths), 23 205 (with 556 infant deaths), 23 094 (with 498 infant deaths), 23 090 (with 497 infant deaths) and 23 297 (with 495 infant deaths) complete cases from SVRS datasets for each respective year. METHOD: A linear mixed effects model (LMM) with a quadratic trend over time in the fixed effects part and a nested random intercept, as well as a nested random slope for a linear trend over time in the part of the random effect, was implemented to describe the situation. This model was selected based on two popular selection criteria: Akaike Information Criterion (AIC) and Bayesian Information Criterion (BIC). RESULTS: The LMMs analysis results demonstrated statistically significant variations in IMR across different districts and over time. Examining the district-specific area under the logarithm of the IMR curves yielded valuable insights into the disparities in IMR among different districts and regions. Furthermore, a significant inverse relationship was observed between IMR and life expectancy at birth, underscoring the significance of mitigating IMR as a means to enhance population health outcomes. CONCLUSION: This study accentuates district-wise and temporal variability when modelling IMR data and highlights regional heterogeneity in infant mortality rates in Bangladesh. Area-based programmes should be created for mothers residing in locations with a higher risk of IMR. Further research can examine socioeconomic elements generating these discrepancies.

Quality attributes of the developed banana flour: Effects of drying methods.

The study aims to investigate the effects of different drying methods on the changes in functional properties, physicochemical composition, bioactive compounds, antioxidant activity, sensory attributes, and microstructural quality of the banana flours. Two local banana cultivars, Mehersagar and Sabri, were dried to produce flour using four distinct drying methods: freeze drying (FD), cabinet drying (CD), microwave oven drying (MOD), and forced air oven drying (FOD). The functional properties of the developed banana flours were observed where the findings were as water holding capacity (0.93 ± 0.06-2.74 ± 0.04 g water/g dry sample), oil absorption capacity (0.87 ± 0.06-2.22 ± 0.10 g oil/g dry sample), swelling capacity (4.62 ± 0.02-5.05 ± 0.03 g paste/g dry sample), bulk density (0.54 ± 0.04-0.81 ± 0.02 g/ml), tapped density (0.62 ± 0.04-0.93 ± 0.03 g/ml) and Carr's Index (9.38 ± 0.47-13.58 ± 0.43%). Freeze-dried Mehersagar cultivar's flour showed the leading functional properties with good flowability and cohesiveness. The physicochemical parameters of the flours also revealed significant differences (p < 0.05) in lightness (L*) (50.51 ± 1.49-72.21 ± 1.05), moisture content (3.96 ± 0.09-7.74 ± 0.13%), protein (2.72 ± 0.07-3.93 ± 0.06%), crude fat (0.11 ± 0.01-0.36 ± 0.04%), crude fiber (0.64 ± 0.03-1.22 ± 0.03%), carbohydrate (84.15 ± 0.24-88.26 ± 0.15%) and energy content (354.25 ± 0.57-370.02 ± 0.39 kcal/g). Total flavonoid content (21.44 ± 0.04-34.34 ± 0.03 mgQE/100g) and phenolic content (29.91 ± 0.01-71.46 ± 0.03 mgGAE/100g) was observed, while the highest retention of bioactive compounds was exhibited in Mehersagar cultivar's flour. In terms of appearance, fineness, taste, flavor, color, and overall acceptability, the dried banana flour of both the cultivars obtained from freeze-dried scored overall acceptability 8.04 ± 0.02 and 7.92 ± 0.17, respectively. The scanning electron microscopy analysis of the microstructure of flour granules from each sample revealed a diverse morphological configuration in particle size and shape. According to the findings of this study, the freeze-drying technology is superior to others, and the Mehersagar banana cultivar is more satisfactory in terms of quality characteristics. Moreover, the quality parameters of banana flour may facilitate the formulation of different flour-based gluten-free baked products and food supplements.

Fire retardant properties enhancement of cotton twill fabric using pumpkin (Cucurbita maxima) extract.

Health hazards and environmental pollution are major concerns in present world. So, it is high time to think about ecofriendly and sustainable production. In this study, pumpkin juice has been used as an ecofriendly flame retardant finish to enhance the functionality of cotton twill fabric. The pumpkin juice extracted from the fresh pumpkin without any chemicals. The cotton fabric was treated with pumpkin juice in exhaust method. The treated and untreated samples were characterized by TGA, FTIR, SEM, and EDX. The flame-retardant property of the samples were evaluated based on the LOI and vertical flame tester. The result demonstrated that the treated samples exhibited high fire-retardant properties after being finished with pumpkin juice. The LOI value of the treated samples increased to 29 from 19 after treatment. The main reason behind the increased flammability is the dehydration of pumpkin juice-treated fabric which was clarified from the TG analysis. Moreover, the FTIR, SEM, and EDX report ensured the presence of bound and unbound water molecules, different salt, and several atoms in the samples treated with pumpkin juice that enhanced the protection against the spreading of the fire and thus improved fire-retardant properties of the treated samples.

Cleaner pathway for developing bioactive textile materials using natural dyes: a review.

Bioactive textile materials are a promising field in the development of functional textiles. The integration of bioactive compounds, such as natural dyes, into textiles offers a range of benefits, including UV protection, anti-microbial properties, and insect repellency. Natural dyes have been shown to have bioactivity, and their integration into textiles has been extensively studied. The application of natural dyes on textile substrates will be an advantage for their inherent functional properties along with their non-toxic and eco-friendly nature. This review addresses the effect of natural dyes on surface modification of most used natural and synthetic fibers and its subsequent effects on their anti-microbial, UV protection and insect repellent properties with natural dyes. Natural dyes have proved to be environmentally friendly in an attempt to improve bioactive functions in textile materials. This review provides a clear view of sustainable resources for the dyeing and finishing of textiles to develop a cleaner pathway of bioactive textiles using natural dyes. Furthermore, the dye source, advantages and disadvantages of natural dye, main dye component, and chemical structure are listed. However, there is still a need for interdisciplinary research to further optimize the integration of natural dyes into textiles and to improve their bioactivity, biocompatibility, and sustainability. The development of bioactive textile materials using natural dyes has the potential to revolutionize the textile industry and to provide a range of benefits to consumers and society.

Impact of textile dyes on health and ecosystem: a review of structure, causes, and potential solutions.

The rapid growth of population and industrialization have intensified the problem of water pollution globally. To meet the challenge of industrialization, the use of synthetic dyes in the textile industry, dyeing and printing industry, tannery and paint industry, paper and pulp industry, cosmetic and food industry, dye manufacturing industry, and pharmaceutical industry has increased exponentially. Among these industries, the textile industry is prominent for the water pollution due to the hefty consumption of water and discharge of coloring materials in the effluent. The discharge of this effluent into the aquatic reservoir affects its biochemical oxygen demand (BOD), chemical oxygen demand (COD), total dissolved solids (TDS), total suspended solids (TSS), and pH. The release of the effluents without any remedial treatment will generate a gigantic peril to the aquatic ecosystem and human health. The ecological-friendly treatment of the dye-containing wastewater to minimize the detrimental effect on human health and the environment is the need of the hour. The purpose of this review is to evaluate the catastrophic effects of textile dyes on human health and the environment. This review provides a comprehensive insight into the dyes and chemicals used in the textile industry, focusing on the typical treatment processes for their removal from industrial wastewaters, including chemical, biological, physical, and hybrid techniques.

A low-density cellulose rich new natural fiber extracted from the bark of jack tree branches and its characterizations.

The demand of natural cellulosic fibers has been increasing day by day due to their versatile uses and eco-friendly nature. The reason behind this demand is due to some unique properties of natural fibers that are suitable for several fibrous applications such as in composite, textile, nano-materials, conductive carbon, biomaterials etc. In this study, a new natural cellulosic fiber is extracted from the bark of the jack tree branches by water retting process. The fiber is characterized by standard methods. The result of the chemical compositions of the fiber shows that it contains α-cellulose 79.32%, hemicellulose 8.01%, lignin 6.77%, ash 3.58% and extractives 2.32%. XRD analysis reveals its high level of crystallinity (86%) and the microfibrillar angle (MFA) calculated from the XRD data is found -29°. The FTIR analysis confirms the presence of expected functional groups. Thermogravimetric analysis (TGA) and the derivative thermogravimetric (DTG) reveal its good thermal stability and the maximum degradation occurred at 358 °C for the degradation of the α-cellulose. The density of the fiber is found 1.05 g/cc, which is lower compared to many other known natural fibers. All these properties of this new fiber are suitable for several sophisticated fibrous applications such as reinforcement in composite, textile, cellulose nano-materials, activated or conductive carbon, biomaterials etc.

Flame Retardant Coatings from Bio-Derived Chitosan, Sodium Alginate, and Metal Salts for Polyamide 66 Textiles.

Bio-derived polysaccharides, namely, chitosan (CS) and sodium alginate (SA) were considered in a layer-by-layer (LbL) deposition to construct flame retardant coatings onto the polyamide 66 (PA66) fabric surfaces. The as-prepared coatings were further modified in the impregnation process with a number of inorganic salts containing boron, nickel, and iron elements. Obtained results revealed that the simultaneously LbL-assembled and metal salt-treated fabric samples exhibited superior flame retardant performance compared to the only LbL-deposited fabric samples. The limiting oxygen index (LOI) value reached up to 25.5% of the CS-SA-iron salt treated fabric sample and the dripping tendency was completely diminished only for the LbL-metal salt modified fabric samples. Among the treated fabric samples, the CS-SA-iron-salt-modified fabric sample exhibited a maximum reduction in the peak heat release rate by 34% and handed improved and higher quality char residues, indicating a possible condensed phase flame retardant mechanism of this applied finishing. Moreover, metal salt-induced cross-linking could enhance the coating stability and durable finishes against regular home laundering where an iron-salt-treated fabric sample could retain anti-dripping properties even up to 10 laundering cycles. Thus, this pairing of bio-macromolecules (i.e., charring agent) with the metal salts in a hybrid system showed efficacy in improving the fire performance of polyamide textiles via the synergistic involvement between them.

Fabrication and investigation of the physico-mechanical properties of Jute-PALF reinforced LLDPE hybrid composites: Effect of gamma irradiation.

The hybridization effect of agro-waste pineapple leaf fibre (PALF) and jute fibre as reinforcement in linear low-density polyethylene (LLDPE) composites was investigated in this work. The samples were fabricated by using the heat press compression moulding. The effect of gamma irradiation on composite physico-mechanical properties was also investigated in order to determine the best gamma dose among 2.50, 5.00, 7.50, and 10.00 kGy. The composite sample containing 40% PALF and 60% jute (with a total weight of 50% fibres) demonstrated the most feasible tensile strength (33.36 ± 0.59 MPa), tensile modulus (1494.41 ± 10.94 MPa), elongation at break (50.92 ± 0.77%), bending strength (82.58 ± 0.49 MPa), bending modulus (4932.46 ± 96.12 MPa), and impact strength (34.38 ± 0.42 kJ/m2) at 7.50 kGy irradiation. Thermogravimetric analysis (TGA) determined the thermal performance of the samples. Scanning electron microscopy (SEM) images at the tensile fracture surfaces of composites revealed the interfacial interaction between reinforcement fibres and matrix.

Mathematical modeling of unsteady flow with uniform/non-uniform temperature and magnetic intensity in a half-moon shaped domain.

The mathematical modeling of two-dimensional unsteady free convective flow and thermal transport inside a half-moon shaped domain charged in the presence of uniform/non-uniform temperature and magnetic effects with Brownian motion of the nanoparticles has been conducted. Thirty-two types of nanofluids in a combination of various nanoparticles and base fluids having different sizes, shapes, and solid concentrations of nanoparticles are chosen to examine the better performance of heat transfer. The circular boundary is cooled while the diameter boundary is heated with uniform/non-uniform temperature. An external uniform/non-uniform/periodic magnetic field is imposed along diameter. The powerful partial differential equations solver, finite element method of Galerkin type, has been engaged in numerical simulation. The numerical solution's heat transfer mechanism reaches a steady state from the unsteady situation within a very short dimensionless time of about 0.65. The thermal transport rate enhances for increasing buoyancy force whereas decreases with higher magnetic intensity. The uniform thermal condition along the diameter of half-moon gives a higher thermal transport rate compared to non-uniform heating conditions. The non-uniform magnetic field provides greater values of the mean Nusselt number than the uniform field. In addition, the outcomes also predict that a better rate of temperature transport for kerosene-based nanofluid than water-based, ethylene glycol-based, and engine oil-based nanofluid. The heat transfer rate is observed at about 67.86 and 23.78% using Co-Kerosene and Co-water nanofluids, respectively, with an additional 1% nanoparticles volume fraction. The blade shape nanoparticles provide a better heat transfer rate than spherical, cylindrical, brick, and platelet shapes. Small size nanoparticles confirm a higher value of average Nusselt number than big size. Mean Nusselt number increases 22.1 and 5.4% using 1% concentrated Cu-water and Cu-engine oil nanofluid, respectively than base fluid.

Fabrication and characterization of stretchable denim fabric using core spun yarn.

This study aimed to investigate the effect of extensibility on cotton blended polyester-spandex core-spun yarn in the weft direction of 3/1 right-handed Z-twill denim. For the preparation of samples, 100% ring spun cotton yarn of 42 tex (14s/1 Ne) was used as warp, and 70:30, 30:70, 60:40, and 40:60 cotton-polyester core-spun yarn of 30 tex (20s/1) was used in the weft direction. Four categories of denim fabric were fabricated by using the air-jet weaving machine. Spandex yarn was used as a core material of weft with a percentage of 2%, 2.5%, 1.9%, and 1.8% respectively. Different physio-mechanical characteristics namely tensile and tearing strength, GSM, growth and recovery percentage, initial modulus, bending length, drape co-efficiency, abrasion resistance, flexural rigidity were evaluated to justify the quality of fabricated pieces of denim. Water wicking and breathability were taken into consideration when determining comfort. Higher cotton containing specimens exhibited lower tensile and tearing strength. Additionally, the produced denim fabrics showed balanced drapability and good breathability.

Exploring factors influencing patient mortality and loss to follow-up in two paediatric hospital wards in Zamfara, North-West Nigeria, 2016-2018.

INTRODUCTION: Child mortality has been linked to infectious diseases, malnutrition and lack of access to essential health services. We investigated possible predictors for death and patients lost to follow up (LTFU) for paediatric patients at the inpatient department (IPD) and inpatient therapeutic feeding centre (ITFC) of the Anka General Hospital (AGH), Zamfara State, Nigeria, to inform best practices at the hospital. METHODS: We conducted a retrospective cohort review study using routinely collected data of all patient admissions to the IPD and ITFC with known hospital exit status between 2016 and 2018. Unadjusted and adjusted rate ratios (aRR) and respective 95% confidence intervals (95% CI) were calculated using Poisson regression to estimate the association between the exposure variables and mortality as well as LTFU. RESULTS: The mortality rate in IPD was 22% lower in 2018 compared to 2016 (aRR 0.78; 95% CI 0.66-0.93) and 70% lower for patients coming from lead-affected villages compared to patients from other villages (aRR 0.30; 95% CI 0.19-0.48). The mortality rate for ITFC patients was 41% higher during rainy season (aRR 1.41; 95% CI 1.2-1.6). LTFU rates in ITFC increased in 2017 and 2018 when compared to 2016 (aRR 1.6; 95% CI 1.2-2.0 and aRR 1.4; 95% CI 1.1-1.8) and patients in ITFC had 2.5 times higher LTFU rates when coming from a lead-affected village. CONCLUSIONS: Our data contributes clearer understanding of the situation in the paediatric wards in AGH in Nigeria, but identifying specific predictors for the multifaceted nature of mortality and LTFU is challenging. Mortality in paediatric patients in IPD of AGH improved during the study period, which is likely linked to better awareness of the hospital, but still remains high. Access to healthcare due to seasonal restrictions contributes to mortalities due to late presentation. Increased awareness of and easier access to healthcare, such as for patients living in lead-affected villages, which are still benefiting from an MSF lead poisoning intervention, decreases mortalities, but increases LTFU. We recommend targeted case audits and qualitative studies to better understand the role of health-seeking behaviour, and social and traditional factors in the use of formal healthcare in this part of Nigeria and potentially similar settings in other countries.

Influence of socio-demographic factors on coverage of full vaccination among children aged 12-23 months: a study in Indian context (2015-2016).

BACKGROUND: Vaccine-preventable diseases (VPDs) are one of the key public health concerns in low and middle-income countries due to incomplete vaccination coverage. Nearly three million children up to 5 years of age die due to VPDs each year. Vaccination plays a significant role in reducing child mortality and morbidity from VPDs. Globally, full vaccination coverage efficiently saves two to three million children's lives from life-threatening VPDs. OBJECTIVE: This study intends to inspect the influence of socio-demographic factors on full vaccination coverage of children aged 12-23 months in India. METHODS: A cross-sectional observational study was carried out using the NFHS-4, 2015-2016 data of India. A total of 44,771 children aged 12-23 months born to the mothers aged 15-49 years in the last 5 years preceding the survey were used for this study. For the analyses of the data, Bivariate and Multivariate analyses were performed. RESULTS: The prevalence of full vaccination coverage of children aged 12-23 months in India was 62%. The result of the study indicated that maternal educational attainment, household wealth status, child size at birth, and maternal health-care services are the main significant predictors of full vaccination coverage. Other socio-demographic factors include maternal age, sex of the household head, exposure to mass media, child birth order, social category, religion, place of residence and region also play significant role in the coverage of full vaccination. CONCLUSION: The study found that socio-demographic factors play a significant role in full vaccination coverage children in India. Therefore, policymaker and administrators should accentuate the inventive approach for the development of women education, improvement of family income, and easy accessibility of maternal and child healthcare services to surmount the impediment of children full vaccination coverage, which eventually reduce the risk of child morbidity and mortality.

Heatline visualization of MHD natural convection heat transfer of nanofluid in a prismatic enclosure.

Temperature transfer by virtue of natural convection for visualizing heat transport characteristics through heatline method within a prismatic cavity filled with Cu-H2O nanofluid considering two different temperature boundary conditions is performed numerically. Two top inclined walls are warmed-up at low temperature whilst the bottom wall is heated two different heated conditions such as uniform temperature condition and linear temperature condition. Two vertical walls are insulated. Finite element technique of Galerkin weighted residual form is employed for solving nonlinear partial differential equations for numerical calculation. Heatlines, isotherm contours, streamline contours, and Nusselt number are employed for displaying numerical simulated results for the model parameters entitled nanoparticles volume fraction, Hartmann number and Rayleigh number. The outcomes indicate that heat transfer rate has a significant impact on thermal boundary condition and shape of the nanoparticles. The temperature transfer value enhances significantly for higher Rayleigh number as well as nanoparticles volume fraction. Hartmann number has a positive impact on fluid flow and temperature transport. The characteristics of heat transport using heatlines method are also performed for predicting the better energy transform compared to isotherm contours. In addition, different types of nanofluids are also employed to examine the best heat transport performance.

Exploitation of seawater for cotton and polyester fabrics colouration.

Water is the ultimate and mostly used media during textile materials processing, especially in colouration. This study investigated the possibilities of using seawater for cotton and polyester fabrics dyeing. Single jersey fabrics made of 100 percent cotton and polyester were dyed using a standard recipe and two separate water source as dyeing mediums. It has been focused on the assessment of colour fastness to wash, perspiration, saliva, rubbing, water, light and colour difference value due to compare the efficiency of dyeing media. The results revealed that the cotton fabric dyed with seawater showed lighter shade than that of ground water sample. But for polyester fabric darker shade was obtained compared to ground water. The cotton sample dyed with sea water carried about 15% higher colour strength than ground water dyed sample but for polyester it was very negligible, only 3%. Moreover, the results of colour fastness to wash, perspiration, saliva, rubbing, water and light for seawater dyed samples of cotton and polyester were shown satisfactory outcomes having the grading of 4-5 in most of the cases. This exploration established that commercial dyeing processes were robust and can be practically transferable into the seawater medium for cotton and polyester fabrics.

Anti-herbivore silicon defences in a model grass are greatest under Miocene levels of atmospheric CO2.

Silicon (Si) has an important role in mitigating diverse biotic and abiotic stresses in plants, mainly via the silicification of plant tissues. Environmental changes such as atmospheric CO2 concentrations may affect grass Si concentrations which, in turn, can alter herbivore performance. We recently demonstrated that pre-industrial atmospheric CO2 increased Si accumulation in Brachypodium distachyon grass, yet the patterns of Si deposition in leaves and whether this affects insect herbivore performance remains unknown. Moreover, it is unclear whether CO2 -driven changes in Si accumulation are linked to changes in gas exchange (e.g. transpiration rates). We therefore investigated how pre-industrial (reduced; rCO2 , 200 ppm), ambient (aCO2 , 410 ppm) and elevated (eCO2 , 640 ppm) CO2 concentrations, in combination with Si-treatment (Si+ or Si-), affected Si accumulation in B. distachyon and its subsequent effect on the performance of the global insect pest, Helicoverpa armigera. rCO2 increased Si concentrations by 29% and 36% compared to aCO2 and eCO2 respectively. These changes were not related to observed changes in gas exchange under different CO2 regimes, however. The increased Si accumulation under rCO2 decreased herbivore relative growth rate (RGR) by 120% relative to eCO2, whereas rCO2 caused herbivore RGR to decrease by 26% compared to eCO2 . Si supplementation also increased the density of macrohairs, silica and prickle cells, which was associated with reduced herbivore performance. There was a negative correlation among macrohair density, silica cell density, prickle cell density and herbivore RGR under rCO2 suggesting that these changes in leaf surface morphology were linked to reduced performance under this CO2 regime. To our knowledge, this is the first study to demonstrate that increased Si accumulation under pre-industrial CO2 reduces insect herbivore performance. Contrastingly, we found reduced Si accumulation under higher CO2 , which suggests that some grasses may become more susceptible to insect herbivores under projected climate change scenarios.