Parent and child expressed affect and children's social competence: modeling direct and indirect pathways.

This study examined links between parents' and children's expressed affect during parent-child play and children's social functioning with peers. A total of 116 kindergarten-age children and their parents (114 mothers, 102 fathers) were observed during physical play interactions and were coded on global measures of expressed positive and negative affect. Kindergarten and 1st-grade teachers and peers provided measures of social competence. Latent variable path analysis with partial least squares was used to examine models that included "direct" and "indirect" pathways. Relations between parental positive affect and children's social competence were mediated by children's expressed positive affect. Parental negative affect was associated with negative social outcomes in children; however, these relations were not mediated by children's negative expressions. The strongest support for the hypothesized models was found in same-sex dyads.

Switching of Candida albicans during successive episodes of recurrent vaginitis.

Strain relatedness and switching were monitored in Candida albicans strains isolated from different body locations through three episodes of recurrent vulvovaginal candidiasis separated by two treatment-latency periods in a single patient. Strain relatedness was assessed by comparing Southern blot hybridization patterns with the relatively immobile mid-repeat sequence Ca3. The following conclusions are demonstrated. (i) Three different strains of C. albicans colonized the mouth, the area under the breasts, and the vulvovaginal, anal, and rectal regions, respectively, at the time of the first infection. (ii) The same strain of C. albicans was responsible for the three vaginal infections. (iii) Switching of colony phenotype occurred with each new vaginal infection. (iv) Enrichment of drug-resistant switch phenotypes (assessed in vitro) was unlikely the basis for the changes in the switch phenotypes of the strain found in the vulvovaginal, anal, and rectal areas after treatment of the first infection with clotrimazole. (v) The same strain of C. albicans was responsible for the recurrent increases in mouth colonization and was distinct from the recurrent vaginal strain. The results of this case study demonstrate the need for further detailed analyses of full-body mycofloras, strain relatedness, switching repertoires, and changes in drug susceptibility during successive episodes of recurrent vulvovaginal candidiasis.