Multidrug resistant and multivirulent avian bacterial pathogens: tackling experimental leg disorders using phytobiotics and antibiotics alone or in combination.

Locomotor disorders caused by multidrug-resistant (MDR) bacterial pathogens denote one of the most detrimental issues that collectively threaten the poultry industry leading to pronounced economic losses across the world. Hence, searching for effective alternatives, especially those extracted from plant origins became of great priority targeting a partial or complete replacement of chemical antimicrobials to tackle their developing resistance. Therefore, we aimed to determine the prevalence and antimicrobial resistance of Staphylococcus aureus (S. aureus), Salmonella species, Mycoplasma synoviae (M. synoviae), and Escherichia coli (E. coli) recovered from 500 broilers and ducks (250 each) with locomotor disorders in various farms in Dakahlia and Sharkia Governorates, Egypt. Additionally, we assessed, for the first time, the in vitro antimicrobial effectiveness of marjoram, garlic, ginger and cinnamon essential oils (EOs) against MDR and multivirulent bacterial isolates as well as the in vivo efficiency of the most effective antibiotics and EOs either separately or in combination in the treatment of experimentally induced poultry leg disorders. The overall prevalence rates of S. aureus, E. coli, Salmonella species, and M. synoviae were 54, 48, 36, and 2%, respectively. Salmonella species and S. aureus prevailed among ducks and broilers (36 and 76%, respectively). Notably, MDR was observed in 100, 91.7, 81.1, and 78.5% of M. synoviae, E. coli, Salmonella, and S. aureus isolates, respectively. Our in vitro results displayed that marjoram was the most forceful EO against MDR and multivirulent chicken vancomycin-resistant S. aureus (VRSA) and duck S. Typhimurium isolates. The current in vivo results declared that marjoram in combination with florfenicol or amoxicillin/clavulanic acid succeeded in relieving the induced duck and chicken leg disorders caused by S. Typhimurium and VRSA, respectively. This was evidenced by improvement in the clinical and histopathological pictures with a reduction of bacterial loads in the experimental birds. Our encountered successful in vitro and in vivo synergistic effectiveness of marjoram combined with florfenicol or amoxicillin/clavulanic acid recommends their therapeutic application for leg disorders and offers opportunities for reducing the antibiotics usage in the poultry industry.

Digital social support for undergraduate students during COVID-19: Pivotal support for the digital transformation.

BACKGROUND: In a time of crisis, such as the COVID-19 pandemic, digital social support can prove helpful in buffering the stress and anxiety especially for students whose lives were being affected both socially and academically. The present study aims to explore the emotional and informational social support available to students during the online transformation of their education. It further investigates factors affecting anxiety and stress among students. DESIGN AND METHODS: An online survey was administered among 479 undergraduate students at a private university in Saudi Arabia from 2nd March till 17th June 2020. Descriptive analysis was used to report the findings of students' self-reported data. RESULTS: The results revealed that more than 70% of the students reported that they were satisfied with the informational and emotional support available to them via digital platforms. However, they felt less connected with the teachers and academic administration. Feeling sick (46%), being locked down at homes and isolation (43%) were major causes of stress and anxiety. 71% of the students reported their ability to keep themselves busy, however, only 55% of students mentioned that they did not feel lonely while confined to their homes. In these circumstances, the continuation of studies via online platforms helped students reduce stress and anxiety (52%). CONCLUSIONS: Although this online transformation was not planned, the university was able to provide adequate informational support to the students. However, students were dependent upon friends, family and relatives for emotional support. The results of the study point towards the need of training for teachers and academic administrative staff in order to better address students' needs for social support during the crisis.  The results also contribute to the body of data reporting the various effects of the COVID-19 pandemic on students in a way to reach a sustainable framework for supporting students.