Valorization and co-treatment of hazardous petroleum refinery oily sludge and sewage associated with bioenergy recovery in tubular microbial fuel cell.

Petroleum refineries generate large amounts of oily sludge which is normally loaded with different residual hazardous petroleum derivatives. Also, the residential complexes affiliated to the petroleum refineries generate considerable volumes of sewage. This study was devoted to investigate the potential of energy recovery from co-bioelectrochemical treatment of petroleum refinery oily sludge (PROS) and sewage using a tubular dual-chambers microbial fuel cell (MFC). Initially, the MFC was operated in a fill and draw mode of 4 cycles, each cycle at a different organic load (OL). The results revealed that maximum removal efficiencies of the organic content as COD were 93.67%, 98.57%, 99.64%, and 99.74%, whereby maximum power outputs were 225 ± 10, 324 ± 7, 1230 ± 18, and 1156 ± 14 mW/m3 for cycle1of OL1 (1138 ± 60 mg/L), cycle2 of OL2 (7000 ± 75 mg/L), cycle3 of OL3 (13,890 ± 50 mg/L), and cycle4 of OL4 (17,100 ± 150 mg/L), respectively. Based on those promising results, the MFC was operated continually for 60 days by feeding the MFC with PROS and sewage at organic loading of 13,000 ± 1000 mg/L. Significant results concerning COD and TPH elimination efficiency > 99.85% and 94.12%, respectively were obtained associated with power output of 1225 ± 25 mW/m3.

Influence of Light Color on Power Generation and Microalgae Growth in Photosynthetic Microbial Fuel Cell with Chlorella Vulgaris Microalgae as Bio-Cathode.

Photosynthetic microbial fuel cell (PMFC) is an environmentally friendly sustainable technique for simultaneous wastewater treatment and power recovery. PMFC utilizes the microalgae to generate oxygen by photosynthesis process in the biocathode. Light sources and intensities have direct effect on chlorophyll pigment formation, photosynthesis processes and microalgae growth. In this study, Chlorella vulgaris was utilized as biocathode in PMFC fed with actual slaughterhouse wastewater. The biocathode was illuminated with florescent light as well as yellow, red and blue LED lights with light intensities of 67.46, 47.03, 26.18 and 4.70 µmol/m.s, respectively. Power output and microalgae growth were considered in evaluating the PMFC performance. Results demonstrated that the highest power output was 217.04 mW/m2 generated under florescent light compared to 28.41, 171.08, and 21.65 mW/m2 observed under yellow, red and blue LEDs, respectively. Additionally, statistical analysis was performed using fifth-degree polynomial model which fitted well the experimental data with a determination coefficient (R2) > 0.97. The results reflected a high confidence level in depicting the growth mechanism of Chlorella vulgaris under lighting sources with different light colors and intensities.

Sustainable application of tubular photosynthesis microbial desalination cell for simultaneous desalination of seawater for potable water supply associated with sewage treatment and energy recovery.

A sustainable approach for simultaneous desalination of actual seawater for potable water supply, and bioelectrochemical treatment of sewage associated with power generation was evaluated in a tubular photosynthesis desalination microbial fuel cell (PDMC) continually operated for 180 days. Anion exchange membrane (AEM) was used to separate the bioanode and desalination compartments, whereby, and cation exchange membrane (CEM) was used to separate the desalination and biocathode compartments. Mixed bacterial species and mixed microalgae were utilized for inoculation of the bioanode and biocathode, respectively. The results revealed that maximum and average desalination efficiencies of saline seawater fed to the desalination compartment were 80 ± 1 % and 72 ± 1.2 %, respectively. Maximum and average removal efficiencies of the sewage organic content in the anodic compartment were up to 99.3 ± 0.5 % and 91.0 ± 0.8 %, respectively associated with maximum power output of 430.7 ± 0.7 mW/m3. In spite of the heavy growth of the mixed bacterial species and microalgae as well, no fouling of AEM and CEM was observed during the entire period of operation. Kinetic study demonstrated that Blackman model described well the bacterial growth. Dense and healthy growth of biofilm and the microalgae in the anodic and cathodic compartments, respectively were clearly observed during the operation period. The promising outcomes of this investigation demonstrated that the suggested approach is a potential sustainable option for simultaneous desalination of saline seawater for potable water supply, biotreatment of sewage, and power generation.

Immobilization of mixed bacteria by novel biocarriers extracted from Cress and Chia seeds for biotreatment of anionic surfactant (SDS)-bearing real wastewaters.

Selection of biocarrier type is an essential element for successful bacterial cells immobilization. The present investigation aimed to evaluate a novel application of Cress and Chia seeds as biocarriers for immobilization of mixed bacterial cells. Being an environmentally friendly, non-polluting, inexpensive, and non-toxic substances makes them promising biocarriers. On the other hand, there is an increasing concern about contamination by surfactants, sodium dodecyl sulfate (SDS) is among the most commonly used surfactant. The Cress and Chia seeds were cross-linked with PVA to prepare two types of beads; CrE-PVA and ChE-PVA, respectively. The beads were utilized for the SDS biodegradation in four kinds of actual SDS-bearing wastewaters originated from; carwash garage (CWW), laundry facility (LWW), and household detergent industry (HWW), in addition to domestic wastewater (DWW). The results revealed that maximum efficiencies of SDS elimination in DWW, LWW, HWW, and CWW were 98.12, 94.32, 93.04, and 99.08%, respectively, using CrE-PVA and 99.04, 94.96, 94.71, and 99.27%, respectively using ChE-PVA. Finally, both types of beads were recycled for five times without losing their stability and efficiency for SDS biodegradation. Four kinetic models were adopted which were Blackman, Monod, Haldane, and Teissier. Results revealed that Teissier model well fitted the experimental data.

New application of gelatin and starch as biocarriers for the biodegradation of reactive blue azo dye using immobilized mixed cells.

Reactive azo dyes, if discharged in unmonitored manner to natural water bodies, can cause remarkable irreversible damage. The current study is centered on the decolorization and biodegradation of reactive blue (RB4) azo dye in an integrated sequential anaerobic-aerobic batch mode process. The biodegradation of reactive blue (RB4) was accomplished using alternatively both starch and gelatin-immobilized mixed cells. Activated sludge freshly collected from a sewage treatment plant was used as the biocatalyst. Complete decolorization of 10 mg/L RB4 under anaerobic conditions observed within 30 h by using individually mixed bacterial cells immobilized with; (1) starch cross-linked with polyvinyl alcohol (PVA-St beads), and (2) gelatin cross-linked with polyvinyl alcohol (PVA-Ge beads). The results revealed that maximum removal efficiencies of chemical oxygen demand (COD) under aerobic conditions were 89, 88, and 86%, using PVA-St beads, whereby they were 90, 85, 84% using PVA-Ge beads occurred within 70, 90 and 100 h at detected concentrations of 10, 20, and 40 mg/L, respectively. The effect of biocarrier has been proven to be negligible as the results were comparable using both bio-carriers.

Bioelectrochemical treatment of actual carwash wastewater associated with sustainable energy generation in three-dimensional microbial fuel cell.

Carwash garages are worldwide cleaning facilities. Discharge of their untreated or improperly treated wastewaters highly contributes to the pollution of water resources. Sodium dodecyl sulfate (SDS), a widely used anionic surfactant in the carwash shampoos represents the major constituent of the carwash wastewater. In this study, a new configuration of three-dimensional MFC packed with irregular shaped graphite granules to support and join the plain anodes in the anodic section. The performance of the 3D-MFC was evaluated in two operational modes; continuous and batch. The evaluation was carried out mainly in terms of the removal efficiency of organic content, in particular SDS as well as oil and grease associated with bioenergy generation from actual carwash wastewater used to fuel the MFC. The results demonstrated that maximum removal efficiencies of COD, SDS, and oil and grease were 87%, 88%, and 90%, respectively. Also, the results demonstrated that during the continuous mode operation maximum current density and power output were 1786 mA/m3 and 482 mW/m3, respectively. At batch operation mode, the maximum current density and power output were 1793 mA/m3 and 478 mW/m3, respectively indicating that the performance of the 3D-MFC was comparable in both operation modes.

Biodegradation of reactive yellow dye using mixed cells immobilized in different biocarriers by sequential anaerobic/aerobic biotreatment: experimental and modelling study.

In this study, the application of immobilized mixed cells for decolourization, biodegradation, and detoxification of reactive yellow dye (RY15) in textile wastewater was investigated via a sequential anaerobic-aerobic process in bench-scale bioreactors and lab-scale bioreactors as well. The mixed cultures were immobilized using three different biocarriers which were sodium alginate (SA), starch (St), and Gelatin (Ge), by the cross-linking with polyvinyl alcohol (PVA). Results revealed that the immobilized cultures had a potential degrading efficiency in the anaerobic and aerobic environment, targeting the initial structure and the formed compounds, respectively. Complete decolourization (100%) of RY15 was observed with a significant chemical oxygen demand (COD) removal, which enhanced the subsequent aerobic phase. Results demonstrated that COD removals were 92% ± 6.8, 96% ± 3.5, and 100%, using PVA-SA, PVA-St, and PVA-Ge at RY15 initial concentrations of 10 mg/L, respectively. The experimental work was extended to investigate the dye biodegradation in real textile wastewater using mixed cells in immobilized in PVA-SA. The Overloading rate (OLR) and Hydraulic retention time (HRT) of the aerobic bioreactor are 24.5 mg/L h and 41.37 h, respectively. The experimental profiles of RY concentration, COD reduction along with biomass growth, were in good agreement with the model predicted profiles. The effectiveness factors were 0.96 and 0.99 for the anaerobic and aerobic phases, respectively.

Sustainable approach for recycling waste lamb and chicken bones for fluoride removal from water followed by reusing fluoride-bearing waste in concrete.

Sustainable management of waste materials is an attractive approach for modern societies. In this study, recycling of raw waste lamb and chicken bones for defluoridation of water has been estimated. The effects of several experimental parameters including contact time, pH, bone dose, fluoride initial concentration, bone grains size, agitation rate, and the effect of co-existing anions in actual samples of wastewater were studied for fluoride removal from aqueous solutions. Results indicated excellent fluoride removal efficiency up to 99.4% and 99.8% using lamb and chicken bones, respectively at fluoride initial concentration of 10 mg F/L and 120 min contact time. Maximum fluoride uptake was obtained at neutral pH range 6-7. Fluoride removal kinetic was well described by the pseudo-second order kinetic model. Both, Langmuir and Freundlich isotherm models could fit the experimental data well with correlation coefficient values >0.99 suggesting favorable conditions of the process. Furthermore, for complete sustainable management of waste bones, the resulted fluoride-bearing sludge was reused in concrete mixes to partially replace sand. Tests of the mechanical properties of fluoride sludge-modified concrete mixes indicated a potential environmentally friendly approach to dispose fluoride sludge in concrete and simultaneously enhance concrete properties.

Desorption of selected PAHs as individuals and as a ternary PAH mixture within a water-soil-nonionic surfactant system.

Remediation of soil contaminated with polycyclic aromatic hydrocarbons (PAHs) is a major environmental concern due to the toxic and carcinogenic properties of these compounds. Desorption and partitioning of anthracene, fluorene and pyrene within soil-aqueous systems in the presence of the nonionic surfactants, Triton X-100 and Tween 80,were studied. The results showed that the addition of Tween 80 solution at 10 g/L initial concentration enhanced the desorption of PAHs by 49.6%, 10.7% and 70.2% for anthracene, fluorene, and pyrene, respectively, from soil into aqueous phase at 72 h equilibration, while the addition of Triton X-100 could enhance the desorption of PAHs from soil by 59.5%, 17.4% and 86.3% for anthracene, fluorene and pyrene, respectively, at similar experimental conditions. The desorption behaviour of the tested PAHs in a ternary mixture was changed relatively since, in the presence of less hydrophobic solutes, the solubility of more hydrophobic solutes was increased leading to a higher desorption rate. The results showed that hydrophobicity is the primary property that controls PAH desorption from soil and surfactant sorption onto the soils (Qm) in which the latter were found to be 3.75 x 10(-6) and 4.82 x 10(-6) mol/g for Triton X-100 and Tween 80, respectively.

Sustainable power generation in continuous flow microbial fuel cell treating actual wastewater: influence of biocatalyst type on electricity production.

Microbial fuel cells (MFCs) have the potential to simultaneously treat wastewater for reuse and to generate electricity. This study mainly considers the performance of an upflow dual-chambered MFC continuously fueled with actual domestic wastewater and alternatively biocatalyzed with aerobic activated sludge and strain of Bacillus Subtilis. The behavior of MFCs during initial biofilm growth and characterization of anodic biofilm were studied. After 45 days of continuous operation, the biofilms on the anodic electrode were well developed. The performance of MFCs was mainly evaluated in terms of COD reductions and electrical power output. Results revealed that the COD removal efficiency was 84% and 90% and the stabilized power outputs were clearly observed achieving a maximum value of 120 and 270 mW/m(2) obtained for MFCs inoculated with mixed cultures and Bacillus Subtilis strain, respectively.

Sorption of quaternary ammonium compounds to municipal sludge.

The sorptive behavior of four quaternary ammonium compounds (QACs) - hexadecyl trimethyl ammonium chloride (C(16)TMA), dodecyl trimethyl ammonium chloride (C(12)TMA), hexadecyl benzyl dimethyl ammonium chloride (C(16)BDMA), and dodecyl benzyl dimethyl ammonium chloride (C(12)BDMA) - to municipal primary, waste activated, mesophilic digested, and thermophilic digested sludges was assessed at 22 degrees C. Batch adsorption of all four separately tested QACs to primary sludge reached equilibrium within 4h. At a nominal, initial QAC concentration of 300mg/L and a sludge volatile solids concentration of 1g/L, the extent of adsorption was 13, 88, 67, and 89% for the C(12)TMA, C(16)TMA, C(12)BDMA, and C(16)BDMA, respectively, and correlated positively to the QAC hydrophobicity and negatively to their critical micelle concentration. Equilibrium partitioning data were described by the Freundlich isotherm model. The adsorption capacity of the four sludges was very similar. In binary QAC mixtures, QACs with relatively high adsorption affinity and at relatively high aqueous concentrations decreased the adsorption of QACs with a low adsorption affinity. At pH 7, about 40% of the sludge-C(12)TMA desorbed, whereas less than 5% of the sludge-C(16)BDMA desorbed in 10 days. The effect of pH was negligible on the desorption extent of C(12)TMA at a pH range 4-10 over 10 days, whereas increasing the solution pH to 10 resulted in more than 50% desorption of C(16)BDMA. Given the fact that approximately 50% of the municipal biosolids are land-applied in the US, the data of this study would help in the assessment of the fate of QACs and their potential effect on human and environmental health.

Influence of sulfate reduction on the microbial dechlorination of pentachloroaniline in a mixed anaerobic culture.

The reductive dechlorination of pentachloroaniline (PCA) was investigated in the absence and presence of sulfate in batch assays using a PCA-dechlorinating mixed anaerobic culture with methanol as the external electron donor at neutral pH and 22 degrees C. PCA at an initial concentration of 7.8 microM was sequentially dechlorinated to dichlorinated anilines in the sulfate-free culture and the culture amended with 300 mg sulfate-S/L. At an initial concentration of 890 mg sulfate-S/L, a higher sulfate reduction rate was achieved, but PCA dechlorination was not observed until the sulfate concentration dropped to about 100 mg S/L. The transient inhibition of PCA is attributed to competition between sulfate reducing and dechlorinating species for electron donor, more likely for H(2) resulting from methanol fermentation. A long-term (118 days) PCA dechlorination assay with the sulfate-amended culture, which included five feeding cycles, resulted in accumulation of both sulfide (886 mg S/L) and acetate (1,900 mg COD/L). Under these conditions, the sulfate reducers were inhibited, while the rate and pathway of PCA dechlorination were not affected. The results of this study show that the rate of sulfate reduction rather than the sulfate concentration alone dictates the outcome of the competition between sulfate reducers and either dechlorinators or methanogens. The findings of the present study have significant implications relative to the fate and transport of PCA and its dechlorination products in sulfate-laden subsurface systems.

Recycling of waste glass as a partial replacement for fine aggregate in concrete.

Waste glass creates serious environmental problems, mainly due to the inconsistency of waste glass streams. With increasing environmental pressure to reduce solid waste and to recycle as much as possible, the concrete industry has adopted a number of methods to achieve this goal. The properties of concretes containing waste glass as fine aggregate were investigated in this study. The strength properties and ASR expansion were analyzed in terms of waste glass content. An overall quantity of 80 kg of crushed waste glass was used as a partial replacement for sand at 10%, 15%, and 20% with 900 kg of concrete mixes. The results proved 80% pozzolanic strength activity given by waste glass after 28 days. The flexural strength and compressive strength of specimens with 20% waste glass content were 10.99% and 4.23%, respectively, higher than those of the control specimen at 28 days. The mortar bar tests demonstrated that the finely crushed waste glass helped reduce expansion by 66% as compared with the control mix.

Reuse of waste iron as a partial replacement of sand in concrete.

One of the major environmental issues in Iraq is the large quantity of waste iron resulting from the industrial sector which is deposited in domestic waste and in landfills. A series of 109 experiments and 586 tests were carried out in this study to examine the feasibility of reusing this waste iron in concrete. Overall, 130 kg of waste iron were reused to partially replace sand at 10%, 15%, and 20% in a total of 1703 kg concrete mixtures. The tests performed to evaluate waste-iron concrete quality included slump, fresh density, dry density, compressive strength, and flexural strength tests: 115 cubes of concrete were molded for the compressive strength and dry density tests, and 87 prisms were cast for the flexural strength tests. This work applied 3, 7, 14, and 28 days curing ages for the concrete mixes. The results confirm that reuse of solid waste material offers an approach to solving the pollution problems that arise from an accumulation of waste in a production site; in the meantime modified properties are added to the concrete. The results show that the concrete mixes made with waste iron had higher compressive strengths and flexural strengths than the plain concrete mixes.

Use of waste plastic in concrete mixture as aggregate replacement.

Industrial activities in Iraq are associated with significant amounts of non-biodegradable solid waste, waste plastic being among the most prominent. This study involved 86 experiments and 254 tests to determine the efficiency of reusing waste plastic in the production of concrete. Thirty kilograms of waste plastic of fabriform shapes was used as a partial replacement for sand by 0%, 10%, 15%, and 20% with 800 kg of concrete mixtures. All of the concrete mixtures were tested at room temperature. These tests include performing slump, fresh density, dry density, compressive strength, flexural strength, and toughness indices. Seventy cubes were molded for compressive strength and dry density tests, and 54 prisms were cast for flexural strength and toughness indices tests. Curing ages of 3, 7, 14, and 28 days for the concrete mixtures were applied in this work. The results proved the arrest of the propagation of micro cracks by introducing waste plastic of fabriform shapes to concrete mixtures. This study insures that reusing waste plastic as a sand-substitution aggregate in concrete gives a good approach to reduce the cost of materials and solve some of the solid waste problems posed by plastics.