ABSTRACT
Immunomodulating properties of ceruloplasmin were determined in vivo on healthy mice line C57BL/6 and mice with Lewis carcinoma. It was established, that ceruloplasmin at the concentration of 1-40 mg/kg stimulated both T- and B--systems of immunity and prevented immunodepression activity of tumor growth.
Subject(s)
Adjuvants, Immunologic/pharmacology , Ceruloplasmin/pharmacology , Lung Neoplasms/immunology , Neoplasms, Experimental/immunology , Animals , B-Lymphocytes/immunology , Concanavalin A/pharmacology , Lymphocyte Activation , Mice , Mice, Inbred C57BL , T-Lymphocytes/immunology , Tumor Cells, Cultured/immunologyABSTRACT
The influence of ceruloplasmin on the development of delayed hypersensitivity (DHS) and activity of T-suppressors were studied in experiments on intact Balb/c mice. Ceruloplasmin introduced in a dose of 5 mg per 1 kg of weight a day before immunization of animals is shown to have a stimulating effect. The amount of the introduced drug being raised to 200 mg per 1 kg of weight suppressed the ability of organism to form DHS effect rather than increased it. Ceruloplasmin is stated to inhibit induction of DHS suppressors and to exert no effect on the expression of functional activity of the formed suppressors.
Subject(s)
Adjuvants, Immunologic , Ceruloplasmin/pharmacology , Hypersensitivity, Delayed/etiology , Animals , Dose-Response Relationship, Drug , Hypersensitivity, Delayed/immunology , Immunization, Passive/methods , Mice , Mice, Inbred BALB C , T-Lymphocytes, Regulatory/drug effects , T-Lymphocytes, Regulatory/immunologyABSTRACT
Total content and individual fractions of polyamines were studied in lymphocytes of thymus and spleen tissues as well as in lymphatic glands of mice with Lewis carcinoma (strain C57BL/6). Fraction composition as well as total content of polyamines were altered during malignization, as a result of which proliferative activity of lymphocytes was shifted.
Subject(s)
Lymphocytes/metabolism , Neoplasms, Experimental/metabolism , Spermidine/metabolism , Spermine/metabolism , Animals , Lymphatic System/metabolism , Mice , Mice, Inbred C57BL , Spleen/metabolism , Thymus Gland/metabolismSubject(s)
Antibodies, Monoclonal/analysis , Antibodies, Viral/analysis , Antigens, Viral/immunology , Epitopes/immunology , Hemagglutinins, Viral/immunology , Influenza A Virus, H2N2 Subtype , Influenza A virus/immunology , Receptors, Virus/immunology , Adsorption , Animals , Antibodies, Monoclonal/isolation & purification , Antibodies, Viral/isolation & purification , Antibody Specificity , Chick Embryo , Immunization , Neutralization Tests , RabbitsABSTRACT
A comparative immunological analysis of the antigenic structure of hemagglutinins of influenza H2N2 viruses isolated in 1957-1967 was carried out by the method of specific adsorption of highly active antiviral rabbit sera. The hemagglutinins of the viruses under study were shown to contain at least three antigenic determinants changing in the course of evolution, one of them shared by all the viruses of the H2N2 subtype remaining antigenically stable since 1957 until 1967.
