Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 7 de 7
Filter
Add more filters










Database
Language
Publication year range
1.
Pharmacol Rep ; 63(3): 733-9, 2011.
Article in English | MEDLINE | ID: mdl-21857084

ABSTRACT

The effects of the sulfhydryl reducing agents 2-mercaptoethanol and dithiothreitol on wortmannin-induced inhibition of phosphoinositide 3-kinase (PI3K) were studied in order to examine whether the sulfhydryl reducing agents directly affect the wortmannin inhibition of PI3K. These reducing agents are commonly used to stabilize enzyme structures by maintaining protein sulfhydryl groups in the reduced state. Preincubation of wortmannin with millimolar levels of 2-mercaptoethanol, a sulfhydryl derivative of ethanol, markedly prevented subsequent wortmannin-induced inhibition of PI3K. In contrast, ethanol, 2-mercaptoethanol lacking sulfhydryl group, and 2-(methylthio)ethanol, a methyl derivative of the sulfhydryl group of 2-mercaptoethanol, had little effect on the wortmannin-induced inhibition of PI3K, which suggests that the prevention of wortmannin-induced inhibition by 2-mercaptoethanol occurs through the sulfhydryl group of this agent. Moreover, dithiothreitol, a second sulfhydryl reducing agent, also markedly prevented wortmannin-induced inhibition of PI3K. These results indicate that the wortmannin-induced inhibition of PI3K is markedly prevented by millimolar concentrations of sulfhydryl reducing agents such as 2-mercaptoethanol and dithiothreitol in the medium, presumably by the binding of wortmannin to the agents.


Subject(s)
Androstadienes/pharmacology , Dithiothreitol/pharmacology , Mercaptoethanol/pharmacology , Phosphoinositide-3 Kinase Inhibitors , Drug Interactions , Enzyme Inhibitors/pharmacology , Humans , Phosphatidylinositol 3-Kinases/metabolism , Sulfhydryl Reagents/pharmacology , Wortmannin
2.
J Pharmacol Sci ; 116(2): 173-80, 2011.
Article in English | MEDLINE | ID: mdl-21597237

ABSTRACT

Oxidative stress is considered a major mediator of arteriosclerosis. In vascular smooth muscle cells, oxidative stress-induced cell death (including apoptosis) is probably related to arterial calcification in arteriosclerosis. Big mitogen-activated protein kinase-1 / extracellular signal-regulated kinase 5 (BMK1/ERK5) is a newly identified member of the mitogen-activated protein kinases family. Like Src tyrosine kinase, BMK1/ERK5 is known to be sensitive to oxidative stress; however, its pathophysiological significance is poorly understood. In this study, we investigated the involvement of BMK1 and Src in H(2)O(2)-induced cell death using cultured rat aortic smooth muscle cells (RASMCs). Cell apoptosis was evaluated by using the TdT-mediated dUTP nick end labeling (TUNEL) method, and BMK1 and Src activities were determined by Western blotting. The main results are as follows: 1) BMK1 and Src were activated by H(2)O(2) in a time- and concentration-dependent manner in RASMCs; 2) BMK1 activation by H(2)O(2) was attenuated both in Src-knockdown RASMCs and in RASMCs pretreated with 4-amino-5-(4-chloro-phenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2), a Src family kinases inhibitor; and 3) H(2)O(2)-induced cell death was increased in BMK1- and Src-knockdown RASMCs as well as in PP2-treated RASMCs. These findings suggested that Src and BMK1 may play defensive and resistive roles against oxidative stress-induced death in RASMCs.


Subject(s)
Aorta/metabolism , Mitogen-Activated Protein Kinase 7/metabolism , Muscle, Smooth, Vascular/metabolism , Oxidative Stress , Animals , Aorta/drug effects , Aorta/enzymology , Base Sequence , Blotting, Western , Cells, Cultured , DNA Primers , Enzyme Activation , Hydrogen Peroxide/pharmacology , Mitogen-Activated Protein Kinase 7/genetics , Muscle, Smooth, Vascular/drug effects , Muscle, Smooth, Vascular/enzymology , RNA, Small Interfering , Rats , src-Family Kinases/antagonists & inhibitors , src-Family Kinases/metabolism
3.
J Pharmacol Sci ; 113(2): 161-8, 2010.
Article in English | MEDLINE | ID: mdl-20508392

ABSTRACT

Clinical studies have shown that angiotensin-receptor blockers (ARBs) reduce the risk of cardiovascular diseases in hypertensive patients. It is assumed that the reduction of the risk by ARBs may be attributed in part to the inhibition of angiotensin II (AII)-induced vascular smooth muscle cell (VSMC) migration associated with atherosclerosis. However, the effect of ARBs on AII-induced changes in intracellular signaling and resultant cell migration has not been well established. Here, we investigated the effect of olmesartan, an ARB, on AII-induced extracellular signal-regulated kinases 1/2 (ERK1/2) and c-Jun N-terminal kinase (JNK) activation and rat aortic smooth muscle cell (RASMC) migration. Olmesartan inhibited AII-induced ERK1/2 and JNK activation at lower concentrations (10 nM). On the other hand, PP2, a Src tyrosine kinase inhibitor, also inhibited AII-induced ERK1/2 and JNK activation, but its effect on ERK1/2 was less pronounced than that of olmesartan. Olmesartan, U0126 (an ERK1/2 inhibitor), SP600125 (a JNK inhibitor), and PP2 potently inhibited AII-induced RASMC migration. From these findings, it was inferred that angiotensin-receptor blockade by olmesartan results in the inhibition of AII-induced activation of Src, ERK1/2, and JNK in RASMC. Olmesartan may be a potent inhibitor of AII-induced VSMC migration, which may be involved in the progression of atherosclerosis.


Subject(s)
Angiotensin II Type 1 Receptor Blockers/pharmacology , Angiotensin II/antagonists & inhibitors , Angiotensin II/pharmacology , Cell Movement/drug effects , Imidazoles/pharmacology , JNK Mitogen-Activated Protein Kinases/metabolism , Mitogen-Activated Protein Kinase 1/metabolism , Mitogen-Activated Protein Kinase 3/metabolism , Mitogen-Activated Protein Kinase 3/physiology , Muscle, Smooth, Vascular/cytology , Muscle, Smooth, Vascular/enzymology , Signal Transduction/physiology , Tetrazoles/pharmacology , src-Family Kinases/metabolism , Animals , Aorta, Thoracic/cytology , Aorta, Thoracic/enzymology , Atherosclerosis/etiology , Atherosclerosis/pathology , Cells, Cultured , Dose-Response Relationship, Drug , Male , Rats , Rats, Sprague-Dawley
4.
Clin Exp Pharmacol Physiol ; 36(12): 1183-5, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19515063

ABSTRACT

1. Pramipexole (PPX), a dopamine D2 and D3 receptor agonist, exerts neuroprotective effects via both dopamine receptor-mediated and non-dopaminergic mechanisms. In the present study, we demonstrate that PPX reduces the toxicity of tunicamycin, a typical endoplasmic reticulum (ER) stressor, in PC12h cells, a subline of PC12 cells. 2. The PC12h cells were treated with 300 micromol / L PPX in the presence of 0.5 micromol / L tunicamycin for 24 h. The neuroprotective effects of PPX against tunicamycin-induced cell death were evaluated using 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays, Hoechst 33258 staining and western blot analysis. 3. Tunicamycin (0.2, 0.3 and 0.5 microg / mL) dose-dependently decreased MTT activity and increased LDH release from PC12h cells. Treatment with 300 micromol / L PPX rescued the tunicamycin-induced decrease in cell viability. 4. Spiperone (10 micromol / L), a dopamine D2 and D4 receptor antagonist, had no effect on PPX neuroprotection against tunicamycin in these cells. Marker proteins of ER stress and apoptosis are known to be upregulated by tunicamycin, but we detected no significant effects of PPX on these factors. 5. In conclusion, we speculate that a combination of several mechanisms may be involved in PPX-induced neuroprotection.


Subject(s)
Benzothiazoles/pharmacology , Cell Death/drug effects , Dopamine Agonists/pharmacology , Neuroprotective Agents/pharmacology , Tunicamycin/adverse effects , Animals , Apoptosis/drug effects , Dopamine Antagonists/pharmacology , Endoplasmic Reticulum/metabolism , L-Lactate Dehydrogenase/metabolism , Oxidative Stress/drug effects , PC12 Cells , Pramipexole , Rats , Spiperone/pharmacology , Tetrazolium Salts/metabolism , Thiazoles/metabolism
5.
Biochem Biophys Res Commun ; 377(2): 550-555, 2008 Dec 12.
Article in English | MEDLINE | ID: mdl-18854177

ABSTRACT

Persistent endoplasmic reticulum (ER) stress and impairment of the ubiquitin-proteasome system (UPS) cause neuronal cell death. However, the relationship between these two phenomena remains controversial. In our current study, we have utilized an expanded polyglutamine fusion protein (polyQ81) expression system in PC12 cells to further examine the involvement of ER stress and UPS impairment in cell death. The expression of polyQ81-induced ER stress and cell death. PolyQ81 also induced the activation of c-Jun N-terminal kinase (JNK) and caspase-3 and an increase in polyubiquitin immunoreactivity, suggesting UPS impairment. ER stress was induced prior to the accumulation of polyubiquitinated proteins. Low doses of lactacystin had almost similar effects on cell viability and on the activation of JNK and caspase-3 between normal cells and polyQ81-expressing cells. These results suggest that ER stress mediates polyglutamine toxicity prior to UPS impairment during the initial stages of these toxic effects.


Subject(s)
Apoptosis , Endoplasmic Reticulum/metabolism , Peptides/metabolism , Ubiquitination , Acetylcysteine/analogs & derivatives , Acetylcysteine/pharmacology , Animals , Caspase 3/metabolism , Cell Survival , Cysteine Proteinase Inhibitors/pharmacology , Enzyme Activation , JNK Mitogen-Activated Protein Kinases/metabolism , PC12 Cells , Peptides/genetics , Polyubiquitin/metabolism , Proteasome Endopeptidase Complex/drug effects , Proteasome Endopeptidase Complex/metabolism , Protein Folding , Rats , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Signal Transduction
6.
Neurosci Lett ; 392(1-2): 101-4, 2006 Jan 09.
Article in English | MEDLINE | ID: mdl-16219421

ABSTRACT

Although many kinds of nicotinic acetylcholine receptor (nAChR) subtypes have been reported in the neuronal tissues, subtype differences in the nAChR-mediated intracellular signaling remains obscure. Using nAChR agonists and antagonists, the involvement of nAChRs in extracellular signal-regulated protein kinase (ERK) phosphorylation in PC12h cells was investigated. Cytisine and nicotine induced the phosphorylation of ERKs in a dose-dependent manner, whereas RJR-2403 had no effect. Cytisine, but not RJR-2403, also induced phosphorylation of CREB. Mecamylamine, dextromethorphan and 18-methoxycoronaridine inhibited nicotine-induced ERK phosphorylation with much higher affinity than dihydro-beta-erythroidine and alpha-conotoxin MII. These results suggest the involvement of alpha3beta4 nAChRs in ERK phosphorylation in PC12h cells.


Subject(s)
Extracellular Signal-Regulated MAP Kinases/metabolism , Nicotine/pharmacology , Receptors, Nicotinic/metabolism , Alkaloids/pharmacology , Animals , Azocines/pharmacology , Cyclic AMP Response Element-Binding Protein/metabolism , Dose-Response Relationship, Drug , Drug Interactions , Nicotinic Agonists/pharmacology , Nicotinic Antagonists/pharmacology , PC12 Cells , Phosphorylation/drug effects , Protein Subunits/metabolism , Quinolizines/pharmacology , Rats
7.
Biochem Biophys Res Commun ; 324(4): 1406-12, 2004 Nov 26.
Article in English | MEDLINE | ID: mdl-15504370

ABSTRACT

Wortmannin caused normal and strong inhibition on catecholamine secretion from bovine adrenal chromaffin cells and in vitro phosphoinositide 3-kinase activity in NaCl-, Na isethionate-, choline Cl-, Na acetate-, and N-acetyl glycine-based media. However, brief preincubation of wortmannin with the media containing amino compounds such as glutamate, aspartate, lysine, and glycine resulted in the prevention of the inhibitory effects of wortmannin on the above responses as two indexes of wortmannin activities. On the other hand, the amino compounds also caused several rapid changes in wortmannin medium; the changes in absorption spectrum of the medium; and the changes in the retention time of the peak on the HPLC chromatogram using a reverse-phase C-18 column and in the pattern of absorption spectrum of the peak. These changes were not observed in the cases of NaCl, Na isethionate, choline Cl, Na acetate or N-acetyl glycine. Another amino compound Tris, which was commonly used as a pH buffer, was unique in time course and induced the slow but parallel changes and reached maximal up to about 24h. These results taken together indicate that the amino compounds markedly inhibit the activities of wortmannin presumably through the binding of wortmannin to amino group.


Subject(s)
Amines/pharmacology , Androstadienes/antagonists & inhibitors , Enzyme Inhibitors/pharmacology , Amino Acids/pharmacology , Androstadienes/chemistry , Androstadienes/pharmacology , Animals , Catecholamines/metabolism , Cattle , Chromaffin Cells/drug effects , Chromaffin Cells/metabolism , Culture Media/chemistry , Enzyme Inhibitors/chemistry , Phosphatidylinositol 3-Kinases/metabolism , Tromethamine/pharmacology , Wortmannin
SELECTION OF CITATIONS
SEARCH DETAIL
...